Experiment: Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1857 and BT1858 are separated by 90 nucleotides BT1858 and BT1859 are separated by 5 nucleotides BT1859 and BT1860 are separated by 42 nucleotides
BT1857: BT1857 - 3-isopropylmalate dehydrogenase (NCBI ptt file), at 2,329,929 to 2,330,990
BT1857
BT1858: BT1858 - (R)-citramalate synthase (EC 2.3.1.182) (from data) , at 2,331,081 to 2,332,580
BT1858
BT1859: BT1859 - 3-isopropylmalate dehydratase small subunit (NCBI ptt file), at 2,332,586 to 2,333,188
BT1859
BT1860: BT1860 - 3-isopropylmalate dehydratase large subunit (NCBI ptt file), at 2,333,231 to 2,334,625
BT1860
Position (kb)
2331
2332
2333 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2330.100 kb on - strand, within BT1857 at 2330.100 kb on - strand, within BT1857 at 2330.104 kb on - strand, within BT1857 at 2330.181 kb on + strand, within BT1857 at 2330.189 kb on + strand, within BT1857 at 2330.201 kb on + strand, within BT1857 at 2330.273 kb on + strand, within BT1857 at 2330.345 kb on + strand, within BT1857 at 2330.346 kb on - strand, within BT1857 at 2330.348 kb on - strand, within BT1857 at 2330.471 kb on - strand, within BT1857 at 2330.519 kb on - strand, within BT1857 at 2330.531 kb on - strand, within BT1857 at 2330.538 kb on - strand, within BT1857 at 2330.544 kb on - strand at 2330.640 kb on - strand, within BT1857 at 2330.640 kb on - strand, within BT1857 at 2330.769 kb on + strand, within BT1857 at 2330.812 kb on + strand, within BT1857 at 2330.813 kb on - strand, within BT1857 at 2330.920 kb on - strand at 2330.920 kb on - strand at 2330.926 kb on - strand at 2330.982 kb on - strand at 2331.031 kb on + strand at 2331.032 kb on - strand at 2331.044 kb on - strand at 2331.067 kb on - strand at 2331.179 kb on - strand at 2331.192 kb on + strand at 2331.193 kb on - strand at 2331.193 kb on - strand at 2331.193 kb on - strand at 2331.193 kb on - strand at 2331.215 kb on + strand at 2331.234 kb on - strand, within BT1858 at 2331.267 kb on - strand, within BT1858 at 2331.274 kb on - strand, within BT1858 at 2331.319 kb on + strand, within BT1858 at 2331.319 kb on + strand, within BT1858 at 2331.319 kb on + strand, within BT1858 at 2331.364 kb on - strand, within BT1858 at 2331.463 kb on - strand, within BT1858 at 2331.476 kb on + strand, within BT1858 at 2331.476 kb on + strand, within BT1858 at 2331.477 kb on - strand, within BT1858 at 2331.593 kb on + strand, within BT1858 at 2331.599 kb on + strand, within BT1858 at 2331.599 kb on + strand, within BT1858 at 2331.631 kb on + strand, within BT1858 at 2331.825 kb on - strand, within BT1858 at 2332.039 kb on + strand, within BT1858 at 2332.040 kb on - strand, within BT1858 at 2332.044 kb on + strand, within BT1858 at 2332.088 kb on + strand, within BT1858 at 2332.088 kb on + strand, within BT1858 at 2332.089 kb on - strand, within BT1858 at 2332.089 kb on - strand, within BT1858 at 2332.089 kb on - strand, within BT1858 at 2332.091 kb on - strand, within BT1858 at 2332.091 kb on - strand, within BT1858 at 2332.138 kb on + strand, within BT1858 at 2332.138 kb on + strand, within BT1858 at 2332.181 kb on - strand, within BT1858 at 2332.182 kb on + strand, within BT1858 at 2332.182 kb on + strand, within BT1858 at 2332.182 kb on + strand, within BT1858 at 2332.222 kb on - strand, within BT1858 at 2332.229 kb on + strand, within BT1858 at 2332.229 kb on + strand, within BT1858 at 2332.232 kb on + strand, within BT1858 at 2332.296 kb on - strand, within BT1858 at 2332.332 kb on + strand, within BT1858 at 2332.350 kb on - strand, within BT1858 at 2332.588 kb on - strand at 2332.588 kb on - strand at 2332.646 kb on + strand at 2332.724 kb on - strand, within BT1859 at 2332.833 kb on + strand, within BT1859 at 2332.884 kb on - strand, within BT1859 at 2332.884 kb on - strand, within BT1859 at 2332.983 kb on + strand, within BT1859 at 2332.984 kb on - strand, within BT1859 at 2333.032 kb on + strand, within BT1859 at 2333.056 kb on + strand, within BT1859 at 2333.056 kb on + strand, within BT1859 at 2333.110 kb on + strand, within BT1859 at 2333.122 kb on + strand, within BT1859 at 2333.126 kb on + strand, within BT1859 at 2333.127 kb on - strand, within BT1859 at 2333.156 kb on - strand at 2333.163 kb on - strand at 2333.206 kb on - strand at 2333.214 kb on - strand at 2333.308 kb on + strand at 2333.309 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml remove 2,330,100 - BT1857 0.16 -4.0 2,330,100 - BT1857 0.16 -2.2 2,330,104 - BT1857 0.16 -4.5 2,330,181 + BT1857 0.24 -2.3 2,330,189 + BT1857 0.24 -6.0 2,330,201 + BT1857 0.26 -4.1 2,330,273 + BT1857 0.32 -3.6 2,330,345 + BT1857 0.39 -2.0 2,330,346 - BT1857 0.39 -3.5 2,330,348 - BT1857 0.39 -2.9 2,330,471 - BT1857 0.51 -0.4 2,330,519 - BT1857 0.56 -6.2 2,330,531 - BT1857 0.57 -6.5 2,330,538 - BT1857 0.57 -6.1 2,330,544 - -0.8 2,330,640 - BT1857 0.67 +1.2 2,330,640 - BT1857 0.67 -2.7 2,330,769 + BT1857 0.79 -2.8 2,330,812 + BT1857 0.83 -0.8 2,330,813 - BT1857 0.83 -1.7 2,330,920 - -4.2 2,330,920 - -3.2 2,330,926 - -5.5 2,330,982 - -3.2 2,331,031 + -3.4 2,331,032 - +0.1 2,331,044 - -1.1 2,331,067 - -1.7 2,331,179 - -3.5 2,331,192 + -3.9 2,331,193 - -7.0 2,331,193 - -2.2 2,331,193 - -2.6 2,331,193 - -0.8 2,331,215 + -3.7 2,331,234 - BT1858 0.10 -3.5 2,331,267 - BT1858 0.12 -2.4 2,331,274 - BT1858 0.13 -2.4 2,331,319 + BT1858 0.16 -2.3 2,331,319 + BT1858 0.16 -2.3 2,331,319 + BT1858 0.16 -1.8 2,331,364 - BT1858 0.19 -3.9 2,331,463 - BT1858 0.25 -4.6 2,331,476 + BT1858 0.26 -1.8 2,331,476 + BT1858 0.26 -6.3 2,331,477 - BT1858 0.26 -3.8 2,331,593 + BT1858 0.34 -6.1 2,331,599 + BT1858 0.35 -2.6 2,331,599 + BT1858 0.35 -4.7 2,331,631 + BT1858 0.37 -1.8 2,331,825 - BT1858 0.50 -1.4 2,332,039 + BT1858 0.64 -2.4 2,332,040 - BT1858 0.64 -3.1 2,332,044 + BT1858 0.64 -0.4 2,332,088 + BT1858 0.67 -3.1 2,332,088 + BT1858 0.67 -2.3 2,332,089 - BT1858 0.67 -2.0 2,332,089 - BT1858 0.67 -2.5 2,332,089 - BT1858 0.67 -1.8 2,332,091 - BT1858 0.67 -2.0 2,332,091 - BT1858 0.67 -3.1 2,332,138 + BT1858 0.70 -4.3 2,332,138 + BT1858 0.70 -5.6 2,332,181 - BT1858 0.73 -2.7 2,332,182 + BT1858 0.73 -5.2 2,332,182 + BT1858 0.73 -4.4 2,332,182 + BT1858 0.73 -1.4 2,332,222 - BT1858 0.76 -3.9 2,332,229 + BT1858 0.77 -2.8 2,332,229 + BT1858 0.77 -2.6 2,332,232 + BT1858 0.77 -2.9 2,332,296 - BT1858 0.81 -3.7 2,332,332 + BT1858 0.83 -5.4 2,332,350 - BT1858 0.85 -2.5 2,332,588 - -6.4 2,332,588 - -4.3 2,332,646 + -4.3 2,332,724 - BT1859 0.23 -2.5 2,332,833 + BT1859 0.41 -5.2 2,332,884 - BT1859 0.49 -4.2 2,332,884 - BT1859 0.49 -1.7 2,332,983 + BT1859 0.66 -5.4 2,332,984 - BT1859 0.66 -1.7 2,333,032 + BT1859 0.74 -2.2 2,333,056 + BT1859 0.78 -3.0 2,333,056 + BT1859 0.78 -2.8 2,333,110 + BT1859 0.87 -5.6 2,333,122 + BT1859 0.89 -0.8 2,333,126 + BT1859 0.90 -2.9 2,333,127 - BT1859 0.90 -2.0 2,333,156 - -1.8 2,333,163 - -6.2 2,333,206 - -1.2 2,333,214 - -1.0 2,333,308 + -1.2 2,333,309 - -6.8
Or see this region's nucleotide sequence