Experiment: RCH2_defined_glucose with Iron (II) 0.635 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04595 and BWI76_RS04600 are separated by 128 nucleotides BWI76_RS04600 and BWI76_RS04605 overlap by 14 nucleotides BWI76_RS04605 and BWI76_RS04610 overlap by 17 nucleotides
BWI76_RS04595: BWI76_RS04595 - DNA-binding transcriptional regulator AraC, at 956,778 to 957,623
_RS04595
BWI76_RS04600: BWI76_RS04600 - inner membrane protein, at 957,752 to 958,519
_RS04600
BWI76_RS04605: BWI76_RS04605 - thiamine ABC transporter ATP-binding protein, at 958,506 to 959,207
_RS04605
BWI76_RS04610: BWI76_RS04610 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 959,191 to 960,801
_RS04610
Position (kb) 958
959
960 Strain fitness (log2 ratio) -2
-1
0
1
2 at 957.710 kb on - strand at 957.711 kb on + strand at 957.882 kb on + strand, within BWI76_RS04600 at 957.910 kb on + strand, within BWI76_RS04600 at 958.400 kb on + strand, within BWI76_RS04600 at 958.852 kb on + strand, within BWI76_RS04605 at 959.409 kb on - strand, within BWI76_RS04610 at 959.490 kb on + strand, within BWI76_RS04610 at 959.622 kb on - strand, within BWI76_RS04610 at 959.760 kb on + strand, within BWI76_RS04610 at 959.799 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 960.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Iron (II) 0.635 mg/mL remove 957,710 - -0.7 957,711 + -0.8 957,882 + BWI76_RS04600 0.17 -0.6 957,910 + BWI76_RS04600 0.21 -0.1 958,400 + BWI76_RS04600 0.84 +0.4 958,852 + BWI76_RS04605 0.49 -0.8 959,409 - BWI76_RS04610 0.14 +0.1 959,490 + BWI76_RS04610 0.19 +0.3 959,622 - BWI76_RS04610 0.27 +0.1 959,760 + BWI76_RS04610 0.35 -0.4 959,799 + BWI76_RS04610 0.38 -0.2 959,944 + BWI76_RS04610 0.47 -1.9 959,944 + BWI76_RS04610 0.47 -0.2 960,041 - +2.0
Or see this region's nucleotide sequence