Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1226

Experiment: Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.5 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1225 and BT1226 are separated by 606 nucleotidesBT1226 and BT1227 are separated by 63 nucleotides BT1225: BT1225 - GDP-fucose synthetase (NCBI ptt file), at 1,524,058 to 1,525,128 BT1225 BT1226: BT1226 - putative long-chain-fatty-acid--CoA ligase (NCBI ptt file), at 1,525,735 to 1,527,393 BT1226 BT1227: BT1227 - conserved hypothetical protein (NCBI ptt file), at 1,527,457 to 1,528,605 BT1227 Position (kb) 1525 1526 1527 1528Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1524.845 kb on + strand, within BT1225at 1524.875 kb on - strand, within BT1225at 1525.180 kb on - strandat 1525.239 kb on - strandat 1525.257 kb on + strandat 1525.264 kb on + strandat 1525.264 kb on + strandat 1525.264 kb on + strandat 1525.448 kb on + strandat 1525.456 kb on + strandat 1525.483 kb on + strandat 1525.483 kb on + strandat 1525.483 kb on + strandat 1525.632 kb on - strandat 1525.673 kb on - strandat 1525.752 kb on - strandat 1525.756 kb on + strandat 1525.757 kb on - strandat 1525.770 kb on + strandat 1525.810 kb on - strandat 1525.830 kb on + strandat 1526.054 kb on + strand, within BT1226at 1526.055 kb on - strand, within BT1226at 1526.136 kb on - strand, within BT1226at 1526.242 kb on - strand, within BT1226at 1526.412 kb on + strand, within BT1226at 1526.413 kb on - strand, within BT1226at 1526.413 kb on - strand, within BT1226at 1526.413 kb on - strand, within BT1226at 1526.413 kb on - strand, within BT1226at 1526.417 kb on - strand, within BT1226at 1526.463 kb on + strand, within BT1226at 1526.463 kb on + strand, within BT1226at 1526.661 kb on - strand, within BT1226at 1526.772 kb on + strand, within BT1226at 1526.815 kb on + strand, within BT1226at 1526.939 kb on + strand, within BT1226at 1526.940 kb on - strand, within BT1226at 1526.953 kb on + strand, within BT1226at 1526.954 kb on - strand, within BT1226at 1526.954 kb on - strand, within BT1226at 1527.140 kb on - strand, within BT1226at 1527.320 kb on + strandat 1527.447 kb on + strandat 1527.464 kb on + strandat 1527.467 kb on + strandat 1527.476 kb on + strandat 1527.602 kb on + strand, within BT1227at 1527.682 kb on - strand, within BT1227at 1527.726 kb on + strand, within BT1227at 1527.783 kb on - strand, within BT1227at 1527.840 kb on + strand, within BT1227at 1527.884 kb on + strand, within BT1227at 1527.884 kb on + strand, within BT1227at 1527.884 kb on + strand, within BT1227at 1528.194 kb on + strand, within BT1227at 1528.244 kb on + strand, within BT1227at 1528.275 kb on + strand, within BT1227at 1528.367 kb on - strand, within BT1227

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.5 mg/ml
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1,524,845 + BT1225 0.73 +1.0
1,524,875 - BT1225 0.76 +1.5
1,525,180 - -2.5
1,525,239 - -1.7
1,525,257 + +0.3
1,525,264 + -1.7
1,525,264 + +2.5
1,525,264 + +0.7
1,525,448 + +0.1
1,525,456 + +0.9
1,525,483 + -0.8
1,525,483 + -0.3
1,525,483 + -0.8
1,525,632 - -2.1
1,525,673 - -1.7
1,525,752 - +0.0
1,525,756 + +1.8
1,525,757 - -1.5
1,525,770 + +4.2
1,525,810 - +1.5
1,525,830 + +2.0
1,526,054 + BT1226 0.19 +2.0
1,526,055 - BT1226 0.19 +1.4
1,526,136 - BT1226 0.24 +1.4
1,526,242 - BT1226 0.31 +1.5
1,526,412 + BT1226 0.41 -1.5
1,526,413 - BT1226 0.41 +2.9
1,526,413 - BT1226 0.41 +0.9
1,526,413 - BT1226 0.41 -0.9
1,526,413 - BT1226 0.41 -0.5
1,526,417 - BT1226 0.41 -0.4
1,526,463 + BT1226 0.44 +2.7
1,526,463 + BT1226 0.44 +0.1
1,526,661 - BT1226 0.56 -0.7
1,526,772 + BT1226 0.63 +1.5
1,526,815 + BT1226 0.65 +3.0
1,526,939 + BT1226 0.73 +0.2
1,526,940 - BT1226 0.73 +2.7
1,526,953 + BT1226 0.73 +1.6
1,526,954 - BT1226 0.73 +1.1
1,526,954 - BT1226 0.73 +0.7
1,527,140 - BT1226 0.85 +0.4
1,527,320 + +1.2
1,527,447 + +0.3
1,527,464 + +0.5
1,527,467 + -0.1
1,527,476 + -0.8
1,527,602 + BT1227 0.13 -0.1
1,527,682 - BT1227 0.20 -1.1
1,527,726 + BT1227 0.23 -1.4
1,527,783 - BT1227 0.28 -1.4
1,527,840 + BT1227 0.33 +2.5
1,527,884 + BT1227 0.37 +0.5
1,527,884 + BT1227 0.37 -0.4
1,527,884 + BT1227 0.37 +2.5
1,528,194 + BT1227 0.64 -1.4
1,528,244 + BT1227 0.68 -1.4
1,528,275 + BT1227 0.71 -0.9
1,528,367 - BT1227 0.79 -1.5

Or see this region's nucleotide sequence