Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20020

Experiment: RCH2_defined_glucose with Iron (II) 0.4266 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20015 and BWI76_RS20020 are separated by 137 nucleotidesBWI76_RS20020 and BWI76_RS20025 overlap by 9 nucleotides BWI76_RS20015: BWI76_RS20015 - hypothetical protein, at 4,193,769 to 4,194,785 _RS20015 BWI76_RS20020: BWI76_RS20020 - nucleoside triphosphatase NudI, at 4,194,923 to 4,195,348 _RS20020 BWI76_RS20025: BWI76_RS20025 - HAMP domain-containing histidine kinase, at 4,195,340 to 4,196,755 _RS20025 Position (kb) 4194 4195 4196Strain fitness (log2 ratio) -3 -2 -1 0 1at 4194.512 kb on + strand, within BWI76_RS20015at 4194.824 kb on - strandat 4195.091 kb on + strand, within BWI76_RS20020at 4195.283 kb on + strand, within BWI76_RS20020at 4195.314 kb on - strandat 4195.956 kb on + strand, within BWI76_RS20025at 4196.001 kb on + strand, within BWI76_RS20025at 4196.305 kb on - strand, within BWI76_RS20025

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Iron (II) 0.4266 mg/mL
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4,194,512 + BWI76_RS20015 0.73 +0.3
4,194,824 - -3.1
4,195,091 + BWI76_RS20020 0.39 +0.1
4,195,283 + BWI76_RS20020 0.85 -0.3
4,195,314 - +0.2
4,195,956 + BWI76_RS20025 0.44 +0.3
4,196,001 + BWI76_RS20025 0.47 -0.1
4,196,305 - BWI76_RS20025 0.68 +0.1

Or see this region's nucleotide sequence