Experiment: RCH2_defined_glucose with Iron (II) 0.4266 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00400 and BWI76_RS00405 are separated by 215 nucleotides BWI76_RS00405 and BWI76_RS00410 overlap by 48 nucleotides BWI76_RS00410 and BWI76_RS00415 are separated by 133 nucleotides BWI76_RS00415 and BWI76_RS00420 overlap by 7 nucleotides
BWI76_RS00400: BWI76_RS00400 - glutamine synthetase, at 83,534 to 84,943
_RS00400
BWI76_RS00405: BWI76_RS00405 - hypothetical protein, at 85,159 to 85,369
_RS00405
BWI76_RS00410: BWI76_RS00410 - translational GTPase TypA, at 85,322 to 87,145
_RS00410
BWI76_RS00415: BWI76_RS00415 - glucose-1-phosphatase, at 87,279 to 87,878
_RS00415
BWI76_RS00420: BWI76_RS00420 - hypothetical protein, at 87,872 to 88,732
_RS00420
Position (kb) 85
86
87
88 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 84.616 kb on + strand, within BWI76_RS00400 at 84.953 kb on + strand at 84.961 kb on - strand at 85.041 kb on - strand at 85.289 kb on - strand, within BWI76_RS00405 at 87.087 kb on + strand at 87.269 kb on - strand at 87.414 kb on - strand, within BWI76_RS00415 at 87.492 kb on + strand, within BWI76_RS00415 at 87.523 kb on + strand, within BWI76_RS00415 at 87.543 kb on - strand, within BWI76_RS00415 at 87.604 kb on - strand, within BWI76_RS00415 at 87.614 kb on + strand, within BWI76_RS00415 at 87.772 kb on - strand, within BWI76_RS00415 at 87.796 kb on + strand, within BWI76_RS00415 at 87.804 kb on - strand, within BWI76_RS00415 at 87.882 kb on - strand at 88.062 kb on + strand, within BWI76_RS00420 at 88.140 kb on + strand, within BWI76_RS00420
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Iron (II) 0.4266 mg/mL remove 84,616 + BWI76_RS00400 0.77 -2.9 84,953 + -3.5 84,961 - -5.3 85,041 - -1.9 85,289 - BWI76_RS00405 0.62 +0.6 87,087 + -0.9 87,269 - -1.2 87,414 - BWI76_RS00415 0.23 +0.9 87,492 + BWI76_RS00415 0.35 +1.2 87,523 + BWI76_RS00415 0.41 +1.8 87,543 - BWI76_RS00415 0.44 +0.6 87,604 - BWI76_RS00415 0.54 -0.1 87,614 + BWI76_RS00415 0.56 -0.2 87,772 - BWI76_RS00415 0.82 -0.3 87,796 + BWI76_RS00415 0.86 +0.1 87,804 - BWI76_RS00415 0.88 +0.1 87,882 - +0.1 88,062 + BWI76_RS00420 0.22 -0.3 88,140 + BWI76_RS00420 0.31 -2.1
Or see this region's nucleotide sequence