Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00405

Experiment: RCH2_defined_glucose with Iron (II) 0.4266 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00400 and BWI76_RS00405 are separated by 215 nucleotidesBWI76_RS00405 and BWI76_RS00410 overlap by 48 nucleotides BWI76_RS00400: BWI76_RS00400 - glutamine synthetase, at 83,534 to 84,943 _RS00400 BWI76_RS00405: BWI76_RS00405 - hypothetical protein, at 85,159 to 85,369 _RS00405 BWI76_RS00410: BWI76_RS00410 - translational GTPase TypA, at 85,322 to 87,145 _RS00410 Position (kb) 85 86Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 84.616 kb on + strand, within BWI76_RS00400at 84.953 kb on + strandat 84.961 kb on - strandat 85.041 kb on - strandat 85.289 kb on - strand, within BWI76_RS00405

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Iron (II) 0.4266 mg/mL
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84,616 + BWI76_RS00400 0.77 -2.9
84,953 + -3.5
84,961 - -5.3
85,041 - -1.9
85,289 - BWI76_RS00405 0.62 +0.6

Or see this region's nucleotide sequence