Experiment: Varel_Bryant_medium_Glucose with Ethanol 3.33 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2400 and BT2401 are separated by 34 nucleotides BT2401 and BT2402 overlap by 4 nucleotides
BT2400: BT2400 - DNA-3-methyladenine glycosylase I (NCBI ptt file), at 2,995,904 to 2,996,476
BT2400
BT2401: BT2401 - threonine synthase (NCBI ptt file), at 2,996,511 to 2,997,812
BT2401
BT2402: BT2402 - phosphoglycerate mutase (NCBI ptt file), at 2,997,809 to 2,999,032
BT2402
Position (kb)
2996
2997
2998 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2995.663 kb on - strand at 2995.741 kb on + strand at 2995.773 kb on - strand at 2995.773 kb on - strand at 2995.845 kb on + strand at 2995.845 kb on + strand at 2995.855 kb on + strand at 2995.855 kb on + strand at 2995.922 kb on - strand at 2995.961 kb on + strand at 2996.077 kb on + strand, within BT2400 at 2996.078 kb on - strand, within BT2400 at 2996.147 kb on + strand, within BT2400 at 2996.147 kb on + strand, within BT2400 at 2996.148 kb on - strand, within BT2400 at 2996.149 kb on + strand, within BT2400 at 2996.235 kb on - strand, within BT2400 at 2996.235 kb on - strand, within BT2400 at 2996.416 kb on - strand, within BT2400 at 2996.463 kb on - strand at 2996.465 kb on + strand at 2996.466 kb on + strand at 2996.490 kb on - strand at 2996.490 kb on - strand at 2996.502 kb on - strand at 2996.528 kb on - strand at 2996.654 kb on + strand, within BT2401 at 2996.672 kb on + strand, within BT2401 at 2996.752 kb on + strand, within BT2401 at 2996.752 kb on + strand, within BT2401 at 2996.806 kb on + strand, within BT2401 at 2996.810 kb on + strand, within BT2401 at 2996.811 kb on - strand, within BT2401 at 2996.811 kb on - strand, within BT2401 at 2996.901 kb on - strand, within BT2401 at 2996.910 kb on + strand, within BT2401 at 2996.938 kb on + strand, within BT2401 at 2996.938 kb on + strand, within BT2401 at 2996.956 kb on + strand, within BT2401 at 2996.956 kb on + strand, within BT2401 at 2996.956 kb on + strand, within BT2401 at 2996.957 kb on - strand, within BT2401 at 2997.115 kb on + strand, within BT2401 at 2997.116 kb on - strand, within BT2401 at 2997.116 kb on - strand, within BT2401 at 2997.122 kb on - strand, within BT2401 at 2997.128 kb on - strand, within BT2401 at 2997.128 kb on - strand, within BT2401 at 2997.156 kb on + strand, within BT2401 at 2997.276 kb on - strand, within BT2401 at 2997.343 kb on + strand, within BT2401 at 2997.344 kb on - strand, within BT2401 at 2997.345 kb on + strand, within BT2401 at 2997.346 kb on - strand, within BT2401 at 2997.346 kb on - strand, within BT2401 at 2997.395 kb on + strand, within BT2401 at 2997.486 kb on + strand, within BT2401 at 2997.532 kb on + strand, within BT2401 at 2997.532 kb on + strand, within BT2401 at 2997.533 kb on - strand, within BT2401 at 2997.583 kb on + strand, within BT2401 at 2997.583 kb on + strand, within BT2401 at 2997.589 kb on - strand, within BT2401 at 2997.675 kb on + strand, within BT2401 at 2997.680 kb on + strand, within BT2401 at 2997.681 kb on - strand, within BT2401 at 2997.681 kb on - strand, within BT2401 at 2997.710 kb on + strand at 2997.798 kb on - strand at 2997.805 kb on + strand at 2997.806 kb on - strand at 2997.842 kb on + strand at 2997.890 kb on + strand at 2997.901 kb on - strand at 2997.901 kb on - strand at 2997.952 kb on + strand, within BT2402 at 2997.989 kb on - strand, within BT2402 at 2998.048 kb on - strand, within BT2402 at 2998.058 kb on + strand, within BT2402 at 2998.058 kb on + strand, within BT2402 at 2998.190 kb on + strand, within BT2402 at 2998.195 kb on + strand, within BT2402 at 2998.210 kb on + strand, within BT2402 at 2998.215 kb on + strand, within BT2402 at 2998.215 kb on + strand, within BT2402 at 2998.215 kb on + strand, within BT2402 at 2998.216 kb on - strand, within BT2402 at 2998.216 kb on - strand, within BT2402 at 2998.250 kb on + strand, within BT2402 at 2998.250 kb on + strand, within BT2402 at 2998.298 kb on - strand, within BT2402 at 2998.454 kb on - strand, within BT2402 at 2998.460 kb on - strand, within BT2402 at 2998.471 kb on + strand, within BT2402 at 2998.471 kb on + strand, within BT2402 at 2998.574 kb on - strand, within BT2402 at 2998.618 kb on + strand, within BT2402 at 2998.659 kb on + strand, within BT2402 at 2998.660 kb on - strand, within BT2402 at 2998.660 kb on - strand, within BT2402 at 2998.691 kb on + strand, within BT2402 at 2998.691 kb on + strand, within BT2402 at 2998.692 kb on - strand, within BT2402 at 2998.692 kb on - strand, within BT2402 at 2998.693 kb on + strand, within BT2402 at 2998.693 kb on + strand, within BT2402 at 2998.693 kb on + strand, within BT2402 at 2998.693 kb on + strand, within BT2402 at 2998.693 kb on + strand, within BT2402 at 2998.694 kb on - strand, within BT2402 at 2998.694 kb on - strand, within BT2402 at 2998.694 kb on - strand, within BT2402 at 2998.694 kb on - strand, within BT2402 at 2998.694 kb on - strand, within BT2402 at 2998.744 kb on + strand, within BT2402
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Ethanol 3.33 vol% remove 2,995,663 - -0.4 2,995,741 + -0.2 2,995,773 - -1.1 2,995,773 - +0.0 2,995,845 + -0.5 2,995,845 + +1.2 2,995,855 + -2.3 2,995,855 + +0.0 2,995,922 - -1.1 2,995,961 + -0.4 2,996,077 + BT2400 0.30 +0.0 2,996,078 - BT2400 0.30 +0.1 2,996,147 + BT2400 0.42 +0.8 2,996,147 + BT2400 0.42 +0.1 2,996,148 - BT2400 0.43 -0.1 2,996,149 + BT2400 0.43 -0.7 2,996,235 - BT2400 0.58 +0.2 2,996,235 - BT2400 0.58 -1.2 2,996,416 - BT2400 0.89 -0.3 2,996,463 - -0.2 2,996,465 + +0.6 2,996,466 + +0.0 2,996,490 - -0.4 2,996,490 - +0.1 2,996,502 - +0.9 2,996,528 - -2.1 2,996,654 + BT2401 0.11 -0.4 2,996,672 + BT2401 0.12 -4.2 2,996,752 + BT2401 0.19 -3.5 2,996,752 + BT2401 0.19 -4.2 2,996,806 + BT2401 0.23 -3.5 2,996,810 + BT2401 0.23 -1.6 2,996,811 - BT2401 0.23 -3.2 2,996,811 - BT2401 0.23 -4.5 2,996,901 - BT2401 0.30 -4.1 2,996,910 + BT2401 0.31 -3.2 2,996,938 + BT2401 0.33 -4.8 2,996,938 + BT2401 0.33 -2.1 2,996,956 + BT2401 0.34 -2.9 2,996,956 + BT2401 0.34 -0.7 2,996,956 + BT2401 0.34 -2.0 2,996,957 - BT2401 0.34 -2.1 2,997,115 + BT2401 0.46 -4.8 2,997,116 - BT2401 0.46 -3.1 2,997,116 - BT2401 0.46 -4.0 2,997,122 - BT2401 0.47 -1.7 2,997,128 - BT2401 0.47 -4.4 2,997,128 - BT2401 0.47 -2.4 2,997,156 + BT2401 0.50 -4.3 2,997,276 - BT2401 0.59 -2.4 2,997,343 + BT2401 0.64 -0.7 2,997,344 - BT2401 0.64 -1.9 2,997,345 + BT2401 0.64 -2.3 2,997,346 - BT2401 0.64 -4.1 2,997,346 - BT2401 0.64 -3.3 2,997,395 + BT2401 0.68 -1.6 2,997,486 + BT2401 0.75 -1.9 2,997,532 + BT2401 0.78 -3.5 2,997,532 + BT2401 0.78 -1.0 2,997,533 - BT2401 0.78 -2.0 2,997,583 + BT2401 0.82 -2.8 2,997,583 + BT2401 0.82 -4.8 2,997,589 - BT2401 0.83 -3.2 2,997,675 + BT2401 0.89 -1.4 2,997,680 + BT2401 0.90 -1.2 2,997,681 - BT2401 0.90 -6.5 2,997,681 - BT2401 0.90 -5.0 2,997,710 + -1.2 2,997,798 - -2.4 2,997,805 + -2.3 2,997,806 - -3.6 2,997,842 + -2.2 2,997,890 + -1.2 2,997,901 - -5.9 2,997,901 - -6.5 2,997,952 + BT2402 0.12 -2.4 2,997,989 - BT2402 0.15 -4.5 2,998,048 - BT2402 0.20 -4.0 2,998,058 + BT2402 0.20 -2.9 2,998,058 + BT2402 0.20 -1.9 2,998,190 + BT2402 0.31 -2.7 2,998,195 + BT2402 0.32 -2.9 2,998,210 + BT2402 0.33 -2.7 2,998,215 + BT2402 0.33 -4.7 2,998,215 + BT2402 0.33 -2.2 2,998,215 + BT2402 0.33 -2.9 2,998,216 - BT2402 0.33 -0.4 2,998,216 - BT2402 0.33 -3.5 2,998,250 + BT2402 0.36 -3.2 2,998,250 + BT2402 0.36 -4.9 2,998,298 - BT2402 0.40 -1.6 2,998,454 - BT2402 0.53 -2.9 2,998,460 - BT2402 0.53 -0.9 2,998,471 + BT2402 0.54 -2.5 2,998,471 + BT2402 0.54 -3.5 2,998,574 - BT2402 0.62 -2.7 2,998,618 + BT2402 0.66 -3.7 2,998,659 + BT2402 0.69 -5.1 2,998,660 - BT2402 0.70 -3.5 2,998,660 - BT2402 0.70 -2.6 2,998,691 + BT2402 0.72 -7.0 2,998,691 + BT2402 0.72 -1.9 2,998,692 - BT2402 0.72 -1.2 2,998,692 - BT2402 0.72 -3.5 2,998,693 + BT2402 0.72 -3.3 2,998,693 + BT2402 0.72 -1.6 2,998,693 + BT2402 0.72 -0.4 2,998,693 + BT2402 0.72 -1.6 2,998,693 + BT2402 0.72 -2.1 2,998,694 - BT2402 0.72 -2.8 2,998,694 - BT2402 0.72 -2.4 2,998,694 - BT2402 0.72 -5.2 2,998,694 - BT2402 0.72 -3.6 2,998,694 - BT2402 0.72 -7.3 2,998,744 + BT2402 0.76 -7.0
Or see this region's nucleotide sequence