Experiment: KB with Fusaric 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0201 and Psyr_0202 are separated by 11 nucleotides Psyr_0202 and Psyr_0203 are separated by 92 nucleotides Psyr_0203 and Psyr_0204 overlap by 4 nucleotides
Psyr_0201: Psyr_0201 - ATP-dependent DNA helicase RecG, at 215,460 to 217,535
_0201
Psyr_0202: Psyr_0202 - transcriptional regulator, LysR family, at 217,547 to 218,470
_0202
Psyr_0203: Psyr_0203 - outer membrane transport energization protein TonB, at 218,563 to 219,309
_0203
Psyr_0204: Psyr_0204 - outer membrane transport energization protein ExbD, at 219,306 to 219,731
_0204
Position (kb) 217
218
219 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 216.643 kb on - strand, within Psyr_0201 at 216.692 kb on - strand, within Psyr_0201 at 217.087 kb on + strand, within Psyr_0201 at 217.087 kb on + strand, within Psyr_0201 at 217.156 kb on + strand, within Psyr_0201 at 217.156 kb on + strand, within Psyr_0201 at 217.156 kb on + strand, within Psyr_0201 at 217.157 kb on - strand, within Psyr_0201 at 217.252 kb on + strand, within Psyr_0201 at 217.253 kb on - strand, within Psyr_0201 at 217.548 kb on + strand at 217.548 kb on + strand at 217.549 kb on - strand at 217.549 kb on - strand at 217.553 kb on - strand at 217.553 kb on - strand at 217.802 kb on + strand, within Psyr_0202 at 217.802 kb on + strand, within Psyr_0202 at 217.802 kb on + strand, within Psyr_0202 at 217.802 kb on + strand, within Psyr_0202 at 217.802 kb on + strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.803 kb on - strand, within Psyr_0202 at 217.889 kb on + strand, within Psyr_0202 at 217.890 kb on - strand, within Psyr_0202 at 218.103 kb on + strand, within Psyr_0202 at 218.103 kb on + strand, within Psyr_0202 at 218.103 kb on + strand, within Psyr_0202 at 218.104 kb on - strand, within Psyr_0202 at 218.104 kb on - strand, within Psyr_0202 at 218.104 kb on - strand, within Psyr_0202 at 218.104 kb on - strand, within Psyr_0202 at 218.160 kb on + strand, within Psyr_0202 at 218.160 kb on + strand, within Psyr_0202 at 218.161 kb on - strand, within Psyr_0202 at 218.161 kb on - strand, within Psyr_0202 at 218.175 kb on + strand, within Psyr_0202 at 218.175 kb on + strand, within Psyr_0202 at 218.175 kb on + strand, within Psyr_0202 at 218.175 kb on + strand, within Psyr_0202 at 218.175 kb on + strand, within Psyr_0202 at 218.176 kb on - strand, within Psyr_0202 at 218.176 kb on - strand, within Psyr_0202 at 218.176 kb on - strand, within Psyr_0202 at 218.176 kb on - strand, within Psyr_0202 at 218.176 kb on - strand, within Psyr_0202 at 218.449 kb on - strand at 218.449 kb on - strand at 218.449 kb on - strand at 218.449 kb on - strand at 218.449 kb on - strand at 218.449 kb on - strand at 218.462 kb on - strand at 218.470 kb on + strand at 218.471 kb on - strand at 218.502 kb on + strand at 218.503 kb on - strand at 218.571 kb on + strand at 219.279 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Fusaric 250 ug/ml remove 216,643 - Psyr_0201 0.57 -1.9 216,692 - Psyr_0201 0.59 +0.5 217,087 + Psyr_0201 0.78 -1.4 217,087 + Psyr_0201 0.78 -2.0 217,156 + Psyr_0201 0.82 -0.1 217,156 + Psyr_0201 0.82 -0.3 217,156 + Psyr_0201 0.82 +1.3 217,157 - Psyr_0201 0.82 +1.9 217,252 + Psyr_0201 0.86 +0.7 217,253 - Psyr_0201 0.86 -0.5 217,548 + +1.0 217,548 + -1.2 217,549 - +0.5 217,549 - -1.0 217,553 - -0.4 217,553 - -0.6 217,802 + Psyr_0202 0.28 -2.2 217,802 + Psyr_0202 0.28 -0.9 217,802 + Psyr_0202 0.28 -0.7 217,802 + Psyr_0202 0.28 -1.8 217,802 + Psyr_0202 0.28 -2.5 217,803 - Psyr_0202 0.28 -2.6 217,803 - Psyr_0202 0.28 +0.3 217,803 - Psyr_0202 0.28 -2.3 217,803 - Psyr_0202 0.28 -0.9 217,803 - Psyr_0202 0.28 -2.6 217,803 - Psyr_0202 0.28 -1.3 217,889 + Psyr_0202 0.37 -2.3 217,890 - Psyr_0202 0.37 -2.0 218,103 + Psyr_0202 0.60 -2.7 218,103 + Psyr_0202 0.60 -1.5 218,103 + Psyr_0202 0.60 +0.9 218,104 - Psyr_0202 0.60 -1.9 218,104 - Psyr_0202 0.60 -2.1 218,104 - Psyr_0202 0.60 -2.1 218,104 - Psyr_0202 0.60 -2.9 218,160 + Psyr_0202 0.66 -1.8 218,160 + Psyr_0202 0.66 -0.6 218,161 - Psyr_0202 0.66 -1.8 218,161 - Psyr_0202 0.66 -3.7 218,175 + Psyr_0202 0.68 -1.6 218,175 + Psyr_0202 0.68 +3.7 218,175 + Psyr_0202 0.68 -1.1 218,175 + Psyr_0202 0.68 -1.5 218,175 + Psyr_0202 0.68 -1.9 218,176 - Psyr_0202 0.68 -1.2 218,176 - Psyr_0202 0.68 -1.9 218,176 - Psyr_0202 0.68 -3.0 218,176 - Psyr_0202 0.68 -3.6 218,176 - Psyr_0202 0.68 -3.0 218,449 - -3.6 218,449 - -4.6 218,449 - -2.1 218,449 - -3.3 218,449 - -0.1 218,449 - -4.6 218,462 - -2.9 218,470 + -2.0 218,471 - -0.5 218,502 + -1.9 218,503 - +0.9 218,571 + -2.3 219,279 - -3.1
Or see this region's nucleotide sequence