Experiment: Varel_Bryant_medium_Glucose with Lithocholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2074 and BT2075 are separated by 28 nucleotides BT2075 and BT2076 overlap by 1 nucleotides BT2076 and BT2077 are separated by 18 nucleotides
BT2074: BT2074 - ketol-acid reductoisomerase (NCBI ptt file), at 2,599,081 to 2,600,160
BT2074
BT2075: BT2075 - acyl-ACP thioesterase (NCBI ptt file), at 2,600,189 to 2,600,932
BT2075
BT2076: BT2076 - acetohydroxybutanoate synthase regulatory subunit (EC 2.2.1.6) (from data) , at 2,600,932 to 2,601,495
BT2076
BT2077: BT2077 - acetolactate synthase large subunit (NCBI ptt file), at 2,601,514 to 2,603,211
BT2077
Position (kb)
2600
2601
2602 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2599.957 kb on + strand, within BT2074 at 2599.958 kb on - strand, within BT2074 at 2599.958 kb on - strand, within BT2074 at 2600.087 kb on - strand at 2600.087 kb on - strand at 2600.165 kb on + strand at 2600.181 kb on - strand at 2600.186 kb on - strand at 2600.187 kb on + strand at 2600.188 kb on - strand at 2600.216 kb on + strand at 2600.216 kb on + strand at 2600.216 kb on + strand at 2600.220 kb on - strand at 2600.247 kb on - strand at 2600.319 kb on + strand, within BT2075 at 2600.319 kb on + strand, within BT2075 at 2600.319 kb on + strand, within BT2075 at 2600.320 kb on - strand, within BT2075 at 2600.342 kb on - strand, within BT2075 at 2600.356 kb on - strand, within BT2075 at 2600.381 kb on + strand, within BT2075 at 2600.520 kb on - strand, within BT2075 at 2600.521 kb on + strand, within BT2075 at 2600.521 kb on + strand, within BT2075 at 2600.522 kb on - strand, within BT2075 at 2600.524 kb on - strand, within BT2075 at 2600.710 kb on - strand, within BT2075 at 2600.763 kb on + strand, within BT2075 at 2600.764 kb on - strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.783 kb on - strand, within BT2075 at 2600.904 kb on + strand at 2600.934 kb on - strand at 2601.015 kb on + strand, within BT2076 at 2601.015 kb on + strand, within BT2076 at 2601.015 kb on + strand, within BT2076 at 2601.087 kb on + strand, within BT2076 at 2601.143 kb on - strand, within BT2076 at 2601.156 kb on + strand, within BT2076 at 2601.158 kb on + strand, within BT2076 at 2601.159 kb on - strand, within BT2076 at 2601.208 kb on - strand, within BT2076 at 2601.216 kb on + strand, within BT2076 at 2601.217 kb on - strand, within BT2076 at 2601.218 kb on + strand, within BT2076 at 2601.218 kb on + strand, within BT2076 at 2601.218 kb on + strand, within BT2076 at 2601.218 kb on + strand, within BT2076 at 2601.219 kb on - strand, within BT2076 at 2601.220 kb on + strand, within BT2076 at 2601.220 kb on + strand, within BT2076 at 2601.261 kb on - strand, within BT2076 at 2601.345 kb on + strand, within BT2076 at 2601.351 kb on + strand, within BT2076 at 2601.355 kb on + strand, within BT2076 at 2601.400 kb on + strand, within BT2076 at 2601.466 kb on + strand at 2601.496 kb on - strand at 2601.496 kb on - strand at 2601.496 kb on - strand at 2601.681 kb on + strand at 2601.978 kb on + strand, within BT2077 at 2601.978 kb on + strand, within BT2077 at 2601.991 kb on - strand, within BT2077 at 2601.996 kb on + strand, within BT2077 at 2602.030 kb on + strand, within BT2077 at 2602.031 kb on - strand, within BT2077 at 2602.267 kb on + strand, within BT2077 at 2602.268 kb on - strand, within BT2077 at 2602.268 kb on - strand, within BT2077 at 2602.279 kb on + strand, within BT2077 at 2602.333 kb on - strand, within BT2077 at 2602.368 kb on + strand, within BT2077 at 2602.385 kb on - strand, within BT2077 at 2602.398 kb on + strand, within BT2077 at 2602.401 kb on + strand, within BT2077 at 2602.402 kb on - strand, within BT2077 at 2602.402 kb on - strand, within BT2077 at 2602.491 kb on + strand, within BT2077
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Lithocholic 0.2 mg/ml remove 2,599,957 + BT2074 0.81 -3.6 2,599,958 - BT2074 0.81 -2.1 2,599,958 - BT2074 0.81 -2.0 2,600,087 - -3.2 2,600,087 - -3.3 2,600,165 + -2.8 2,600,181 - -0.0 2,600,186 - +0.3 2,600,187 + -4.1 2,600,188 - +0.1 2,600,216 + -2.8 2,600,216 + -6.5 2,600,216 + -2.4 2,600,220 - +0.4 2,600,247 - +0.3 2,600,319 + BT2075 0.17 -1.2 2,600,319 + BT2075 0.17 -5.7 2,600,319 + BT2075 0.17 -3.9 2,600,320 - BT2075 0.18 +0.0 2,600,342 - BT2075 0.21 -0.2 2,600,356 - BT2075 0.22 +0.3 2,600,381 + BT2075 0.26 -3.1 2,600,520 - BT2075 0.44 -0.4 2,600,521 + BT2075 0.45 -3.3 2,600,521 + BT2075 0.45 -5.6 2,600,522 - BT2075 0.45 -0.0 2,600,524 - BT2075 0.45 +0.5 2,600,710 - BT2075 0.70 -0.7 2,600,763 + BT2075 0.77 -5.8 2,600,764 - BT2075 0.77 +1.8 2,600,782 + BT2075 0.80 -3.1 2,600,782 + BT2075 0.80 -1.6 2,600,782 + BT2075 0.80 -4.4 2,600,783 - BT2075 0.80 +0.2 2,600,904 + -0.0 2,600,934 - -2.0 2,601,015 + BT2076 0.15 -3.7 2,601,015 + BT2076 0.15 -5.4 2,601,015 + BT2076 0.15 -3.3 2,601,087 + BT2076 0.27 -2.6 2,601,143 - BT2076 0.37 -1.8 2,601,156 + BT2076 0.40 -3.8 2,601,158 + BT2076 0.40 -3.0 2,601,159 - BT2076 0.40 -6.2 2,601,208 - BT2076 0.49 -1.0 2,601,216 + BT2076 0.50 -3.0 2,601,217 - BT2076 0.51 -1.0 2,601,218 + BT2076 0.51 -2.8 2,601,218 + BT2076 0.51 -3.0 2,601,218 + BT2076 0.51 -1.4 2,601,218 + BT2076 0.51 -2.7 2,601,219 - BT2076 0.51 -2.2 2,601,220 + BT2076 0.51 -0.0 2,601,220 + BT2076 0.51 -2.2 2,601,261 - BT2076 0.58 -1.0 2,601,345 + BT2076 0.73 -4.1 2,601,351 + BT2076 0.74 -4.5 2,601,355 + BT2076 0.75 -1.9 2,601,400 + BT2076 0.83 -4.2 2,601,466 + -3.0 2,601,496 - -3.6 2,601,496 - -2.2 2,601,496 - -3.6 2,601,681 + -6.0 2,601,978 + BT2077 0.27 -1.0 2,601,978 + BT2077 0.27 -2.8 2,601,991 - BT2077 0.28 -3.3 2,601,996 + BT2077 0.28 -4.9 2,602,030 + BT2077 0.30 -2.6 2,602,031 - BT2077 0.30 -2.8 2,602,267 + BT2077 0.44 -4.6 2,602,268 - BT2077 0.44 -0.0 2,602,268 - BT2077 0.44 -2.9 2,602,279 + BT2077 0.45 -2.9 2,602,333 - BT2077 0.48 -1.8 2,602,368 + BT2077 0.50 -3.6 2,602,385 - BT2077 0.51 -1.4 2,602,398 + BT2077 0.52 -2.4 2,602,401 + BT2077 0.52 -2.4 2,602,402 - BT2077 0.52 -1.8 2,602,402 - BT2077 0.52 -1.6 2,602,491 + BT2077 0.58 -5.7
Or see this region's nucleotide sequence