Experiment: KB with Methanol 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2653 and Psyr_2654 are separated by 29 nucleotides Psyr_2654 and Psyr_2655 are separated by 131 nucleotides Psyr_2655 and Psyr_2656 are separated by 171 nucleotides Psyr_2656 and Psyr_2657 are separated by 143 nucleotides
Psyr_2653: Psyr_2653 - ISPsy8, transposase OrfA, at 3,224,311 to 3,224,826
_2653
Psyr_2654: Psyr_2654 - Integrase, catalytic region, at 3,224,856 to 3,225,662
_2654
Psyr_2655: Psyr_2655 - Nitroreductase, at 3,225,794 to 3,226,615
_2655
Psyr_2656: Psyr_2656 - hypothetical protein, at 3,226,787 to 3,227,137
_2656
Psyr_2657: Psyr_2657 - hypothetical protein, at 3,227,281 to 3,228,120
_2657
Position (kb)
3225
3226
3227 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.718 kb on + strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.719 kb on - strand at 3225.745 kb on + strand at 3225.746 kb on - strand at 3225.778 kb on + strand at 3225.778 kb on + strand at 3225.779 kb on - strand at 3226.067 kb on + strand, within Psyr_2655 at 3226.067 kb on + strand, within Psyr_2655 at 3226.067 kb on + strand, within Psyr_2655 at 3226.068 kb on - strand, within Psyr_2655 at 3226.096 kb on - strand, within Psyr_2655 at 3226.096 kb on - strand, within Psyr_2655 at 3226.096 kb on - strand, within Psyr_2655 at 3226.114 kb on + strand, within Psyr_2655 at 3226.147 kb on - strand, within Psyr_2655 at 3226.147 kb on - strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.166 kb on + strand, within Psyr_2655 at 3226.167 kb on - strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.259 kb on + strand, within Psyr_2655 at 3226.260 kb on - strand, within Psyr_2655 at 3226.347 kb on + strand, within Psyr_2655 at 3226.425 kb on - strand, within Psyr_2655 at 3226.459 kb on - strand, within Psyr_2655 at 3226.459 kb on - strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.461 kb on - strand, within Psyr_2655 at 3226.461 kb on - strand, within Psyr_2655 at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.727 kb on - strand at 3226.727 kb on - strand at 3226.757 kb on + strand at 3226.758 kb on - strand at 3226.758 kb on - strand at 3226.768 kb on - strand at 3226.771 kb on + strand at 3226.771 kb on + strand at 3226.771 kb on + strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.788 kb on + strand at 3226.789 kb on - strand at 3226.841 kb on - strand, within Psyr_2656 at 3226.841 kb on - strand, within Psyr_2656 at 3226.863 kb on + strand, within Psyr_2656 at 3226.938 kb on + strand, within Psyr_2656 at 3226.938 kb on + strand, within Psyr_2656 at 3226.939 kb on - strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.947 kb on - strand, within Psyr_2656 at 3226.947 kb on - strand, within Psyr_2656 at 3227.060 kb on + strand, within Psyr_2656 at 3227.061 kb on - strand, within Psyr_2656 at 3227.061 kb on - strand, within Psyr_2656 at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.204 kb on + strand at 3227.204 kb on + strand at 3227.205 kb on - strand at 3227.205 kb on - strand at 3227.205 kb on - strand at 3227.306 kb on - strand at 3227.306 kb on - strand at 3227.306 kb on - strand at 3227.377 kb on + strand, within Psyr_2657 at 3227.377 kb on + strand at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.421 kb on - strand, within Psyr_2657 at 3227.451 kb on + strand, within Psyr_2657 at 3227.452 kb on - strand, within Psyr_2657 at 3227.503 kb on + strand, within Psyr_2657 at 3227.504 kb on - strand, within Psyr_2657
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methanol 5 vol% remove 3,225,718 + -0.3 3,225,718 + +0.5 3,225,718 + -1.7 3,225,718 + +0.4 3,225,718 + -0.5 3,225,718 + -0.9 3,225,718 + +0.5 3,225,718 + -0.2 3,225,718 + +1.1 3,225,719 - -1.0 3,225,719 - +0.1 3,225,719 - +1.8 3,225,719 - +1.0 3,225,719 - +2.0 3,225,719 - +0.1 3,225,745 + -0.3 3,225,746 - -0.2 3,225,778 + +0.1 3,225,778 + -2.5 3,225,779 - -0.9 3,226,067 + Psyr_2655 0.33 +0.2 3,226,067 + Psyr_2655 0.33 +1.6 3,226,067 + Psyr_2655 0.33 -0.7 3,226,068 - Psyr_2655 0.33 -1.8 3,226,096 - Psyr_2655 0.37 +1.1 3,226,096 - Psyr_2655 0.37 +0.1 3,226,096 - Psyr_2655 0.37 +2.4 3,226,114 + Psyr_2655 0.39 +0.0 3,226,147 - Psyr_2655 0.43 -0.8 3,226,147 - Psyr_2655 0.43 -0.5 3,226,166 + Psyr_2655 0.45 +0.9 3,226,166 + Psyr_2655 0.45 +2.2 3,226,166 + Psyr_2655 0.45 +1.7 3,226,166 + Psyr_2655 0.45 -0.4 3,226,166 + Psyr_2655 0.45 -0.7 3,226,166 + Psyr_2655 0.45 +0.2 3,226,166 + Psyr_2655 0.45 +0.5 3,226,166 + Psyr_2655 0.45 +0.1 3,226,167 - Psyr_2655 0.45 -0.3 3,226,259 + Psyr_2655 0.57 +0.6 3,226,259 + Psyr_2655 0.57 -4.0 3,226,259 + Psyr_2655 0.57 -0.3 3,226,259 + Psyr_2655 0.57 +0.9 3,226,259 + Psyr_2655 0.57 +0.5 3,226,260 - Psyr_2655 0.57 +0.7 3,226,347 + Psyr_2655 0.67 -1.0 3,226,425 - Psyr_2655 0.77 +0.7 3,226,459 - Psyr_2655 0.81 +2.0 3,226,459 - Psyr_2655 0.81 +0.1 3,226,460 + Psyr_2655 0.81 +0.7 3,226,460 + Psyr_2655 0.81 +1.2 3,226,460 + Psyr_2655 0.81 -0.0 3,226,460 + Psyr_2655 0.81 +0.1 3,226,461 - Psyr_2655 0.81 +0.5 3,226,461 - Psyr_2655 0.81 +0.4 3,226,701 + -0.4 3,226,701 + +0.9 3,226,701 + -1.9 3,226,701 + -0.2 3,226,701 + +0.8 3,226,701 + +0.4 3,226,701 + -1.5 3,226,701 + +1.5 3,226,702 - +0.4 3,226,702 - -0.3 3,226,702 - -0.2 3,226,702 - +1.1 3,226,702 - -1.2 3,226,727 - -2.8 3,226,727 - -1.6 3,226,757 + +0.5 3,226,758 - -0.6 3,226,758 - +1.0 3,226,768 - -0.7 3,226,771 + +0.5 3,226,771 + +0.2 3,226,771 + +1.8 3,226,772 - -2.3 3,226,772 - +0.7 3,226,772 - -1.8 3,226,772 - +1.1 3,226,772 - -0.3 3,226,772 - -1.7 3,226,788 + +1.1 3,226,789 - +0.1 3,226,841 - Psyr_2656 0.15 +1.5 3,226,841 - Psyr_2656 0.15 -0.5 3,226,863 + Psyr_2656 0.22 +0.5 3,226,938 + Psyr_2656 0.43 +0.5 3,226,938 + Psyr_2656 0.43 -2.0 3,226,939 - Psyr_2656 0.43 -2.9 3,226,942 + Psyr_2656 0.44 -1.8 3,226,942 + Psyr_2656 0.44 -0.5 3,226,942 + Psyr_2656 0.44 -1.0 3,226,942 + Psyr_2656 0.44 +1.9 3,226,942 + Psyr_2656 0.44 +0.2 3,226,942 + Psyr_2656 0.44 +0.7 3,226,943 - Psyr_2656 0.44 +0.8 3,226,943 - Psyr_2656 0.44 +2.5 3,226,943 - Psyr_2656 0.44 +0.2 3,226,943 - Psyr_2656 0.44 -1.1 3,226,943 - Psyr_2656 0.44 +1.3 3,226,943 - Psyr_2656 0.44 -2.6 3,226,946 + Psyr_2656 0.45 -0.4 3,226,946 + Psyr_2656 0.45 +0.7 3,226,946 + Psyr_2656 0.45 +1.3 3,226,946 + Psyr_2656 0.45 -1.2 3,226,947 - Psyr_2656 0.46 +0.5 3,226,947 - Psyr_2656 0.46 -0.6 3,227,060 + Psyr_2656 0.78 -0.5 3,227,061 - Psyr_2656 0.78 -2.0 3,227,061 - Psyr_2656 0.78 +0.4 3,227,149 + -0.3 3,227,149 + +1.0 3,227,149 + -0.5 3,227,149 + +0.1 3,227,149 + +0.9 3,227,150 - +1.1 3,227,150 - -1.0 3,227,150 - +1.4 3,227,150 - +0.7 3,227,150 - -1.2 3,227,150 - +0.3 3,227,150 - -0.5 3,227,204 + +0.1 3,227,204 + +2.3 3,227,205 - +0.5 3,227,205 - -0.7 3,227,205 - +0.6 3,227,306 - -0.2 3,227,306 - +0.7 3,227,306 - -1.9 3,227,377 + Psyr_2657 0.11 -0.2 3,227,377 + -1.0 3,227,378 - Psyr_2657 0.12 -1.7 3,227,378 - Psyr_2657 0.12 +1.5 3,227,378 - Psyr_2657 0.12 -1.3 3,227,378 - Psyr_2657 0.12 +0.0 3,227,378 - Psyr_2657 0.12 +0.2 3,227,378 - Psyr_2657 0.12 -0.7 3,227,421 - Psyr_2657 0.17 +1.0 3,227,451 + Psyr_2657 0.20 +1.2 3,227,452 - Psyr_2657 0.20 +1.1 3,227,503 + Psyr_2657 0.26 -0.1 3,227,504 - Psyr_2657 0.27 -0.8
Or see this region's nucleotide sequence