Experiment: KB with Methanol 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2418 and Psyr_2419 are separated by 22 nucleotides Psyr_2419 and Psyr_2420 are separated by 154 nucleotides Psyr_2420 and Psyr_2421 are separated by 283 nucleotides
Psyr_2418: Psyr_2418 - shikimate dehydrogenase, at 2,800,692 to 2,801,507
_2418
Psyr_2419: Psyr_2419 - Protein of unknown function DUF480, at 2,801,530 to 2,802,204
_2419
Psyr_2420: Psyr_2420 - conserved hypothetical protein, at 2,802,359 to 2,802,868
_2420
Psyr_2421: Psyr_2421 - Major facilitator superfamily, at 2,803,152 to 2,804,306
_2421
Position (kb)
2801
2802
2803 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2800.550 kb on + strand at 2800.550 kb on + strand at 2800.550 kb on + strand at 2800.550 kb on + strand at 2800.551 kb on - strand at 2800.551 kb on - strand at 2800.551 kb on - strand at 2800.600 kb on + strand at 2800.600 kb on + strand at 2800.600 kb on + strand at 2800.600 kb on + strand at 2800.600 kb on + strand at 2800.600 kb on + strand at 2800.601 kb on - strand at 2800.601 kb on - strand at 2800.601 kb on - strand at 2800.601 kb on - strand at 2800.614 kb on + strand at 2800.615 kb on - strand at 2800.615 kb on - strand at 2800.615 kb on - strand at 2800.639 kb on - strand at 2800.643 kb on + strand at 2800.643 kb on + strand at 2800.644 kb on - strand at 2800.644 kb on - strand at 2800.652 kb on - strand at 2800.652 kb on - strand at 2800.707 kb on - strand at 2800.707 kb on - strand at 2800.720 kb on + strand at 2800.721 kb on - strand at 2800.773 kb on - strand at 2800.783 kb on - strand, within Psyr_2418 at 2800.793 kb on - strand, within Psyr_2418 at 2800.806 kb on + strand, within Psyr_2418 at 2800.807 kb on - strand, within Psyr_2418 at 2800.809 kb on + strand, within Psyr_2418 at 2800.809 kb on + strand, within Psyr_2418 at 2800.810 kb on - strand, within Psyr_2418 at 2800.810 kb on - strand, within Psyr_2418 at 2800.964 kb on + strand, within Psyr_2418 at 2801.010 kb on + strand, within Psyr_2418 at 2801.010 kb on + strand, within Psyr_2418 at 2801.010 kb on + strand, within Psyr_2418 at 2801.010 kb on + strand, within Psyr_2418 at 2801.010 kb on + strand, within Psyr_2418 at 2801.011 kb on - strand, within Psyr_2418 at 2801.011 kb on - strand, within Psyr_2418 at 2801.442 kb on + strand at 2801.442 kb on + strand at 2801.443 kb on - strand at 2801.443 kb on - strand at 2801.473 kb on - strand at 2801.532 kb on - strand at 2801.703 kb on - strand, within Psyr_2419 at 2801.703 kb on - strand, within Psyr_2419 at 2801.703 kb on - strand, within Psyr_2419 at 2801.879 kb on - strand, within Psyr_2419 at 2801.879 kb on - strand, within Psyr_2419 at 2802.328 kb on - strand at 2802.328 kb on - strand at 2802.328 kb on - strand at 2802.329 kb on + strand at 2802.329 kb on + strand at 2802.330 kb on - strand at 2802.353 kb on + strand at 2802.353 kb on + strand at 2802.354 kb on - strand at 2802.354 kb on - strand at 2802.354 kb on - strand at 2802.354 kb on - strand at 2802.364 kb on + strand at 2802.364 kb on + strand at 2802.365 kb on - strand at 2802.365 kb on - strand at 2802.365 kb on - strand at 2802.374 kb on + strand at 2802.594 kb on - strand, within Psyr_2420 at 2802.594 kb on - strand, within Psyr_2420 at 2802.755 kb on + strand, within Psyr_2420 at 2802.756 kb on - strand, within Psyr_2420 at 2802.756 kb on - strand, within Psyr_2420 at 2802.756 kb on - strand, within Psyr_2420 at 2802.767 kb on + strand, within Psyr_2420 at 2802.767 kb on + strand, within Psyr_2420 at 2802.807 kb on - strand, within Psyr_2420 at 2802.917 kb on - strand at 2802.917 kb on - strand at 2802.917 kb on - strand at 2802.934 kb on + strand at 2803.087 kb on + strand at 2803.088 kb on - strand at 2803.088 kb on - strand at 2803.147 kb on + strand at 2803.148 kb on - strand at 2803.148 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methanol 5 vol% remove 2,800,550 + +0.3 2,800,550 + +1.0 2,800,550 + -0.2 2,800,550 + +0.9 2,800,551 - -0.3 2,800,551 - +0.9 2,800,551 - -0.2 2,800,600 + -0.3 2,800,600 + +1.2 2,800,600 + -2.4 2,800,600 + -0.3 2,800,600 + -1.0 2,800,600 + +1.5 2,800,601 - +0.7 2,800,601 - -0.4 2,800,601 - -0.0 2,800,601 - +2.2 2,800,614 + -0.1 2,800,615 - +0.1 2,800,615 - -0.4 2,800,615 - +0.6 2,800,639 - -0.4 2,800,643 + +0.9 2,800,643 + +0.8 2,800,644 - -0.8 2,800,644 - -1.7 2,800,652 - +0.0 2,800,652 - -1.9 2,800,707 - +0.6 2,800,707 - -0.6 2,800,720 + -2.4 2,800,721 - -1.4 2,800,773 - +1.4 2,800,783 - Psyr_2418 0.11 -0.1 2,800,793 - Psyr_2418 0.12 -0.2 2,800,806 + Psyr_2418 0.14 -1.2 2,800,807 - Psyr_2418 0.14 +0.8 2,800,809 + Psyr_2418 0.14 +1.1 2,800,809 + Psyr_2418 0.14 -0.3 2,800,810 - Psyr_2418 0.14 +0.6 2,800,810 - Psyr_2418 0.14 +1.2 2,800,964 + Psyr_2418 0.33 +0.8 2,801,010 + Psyr_2418 0.39 -0.2 2,801,010 + Psyr_2418 0.39 +0.8 2,801,010 + Psyr_2418 0.39 -0.1 2,801,010 + Psyr_2418 0.39 +1.3 2,801,010 + Psyr_2418 0.39 +1.1 2,801,011 - Psyr_2418 0.39 +0.8 2,801,011 - Psyr_2418 0.39 -0.9 2,801,442 + +1.7 2,801,442 + +0.1 2,801,443 - -1.0 2,801,443 - -0.5 2,801,473 - -0.1 2,801,532 - -0.6 2,801,703 - Psyr_2419 0.26 -0.2 2,801,703 - Psyr_2419 0.26 +0.5 2,801,703 - Psyr_2419 0.26 -1.8 2,801,879 - Psyr_2419 0.52 -1.0 2,801,879 - Psyr_2419 0.52 -1.6 2,802,328 - -0.7 2,802,328 - -0.1 2,802,328 - +0.2 2,802,329 + -1.3 2,802,329 + +0.0 2,802,330 - +1.1 2,802,353 + -1.8 2,802,353 + +3.1 2,802,354 - -0.0 2,802,354 - -1.5 2,802,354 - -2.0 2,802,354 - -2.8 2,802,364 + +2.6 2,802,364 + +0.6 2,802,365 - -2.6 2,802,365 - -3.0 2,802,365 - +0.4 2,802,374 + -0.4 2,802,594 - Psyr_2420 0.46 +1.1 2,802,594 - Psyr_2420 0.46 +0.6 2,802,755 + Psyr_2420 0.78 -0.0 2,802,756 - Psyr_2420 0.78 +1.6 2,802,756 - Psyr_2420 0.78 +1.5 2,802,756 - Psyr_2420 0.78 +1.1 2,802,767 + Psyr_2420 0.80 +0.8 2,802,767 + Psyr_2420 0.80 -1.3 2,802,807 - Psyr_2420 0.88 +1.2 2,802,917 - -1.5 2,802,917 - -3.5 2,802,917 - +0.0 2,802,934 + +0.4 2,803,087 + +1.0 2,803,088 - +0.8 2,803,088 - +1.9 2,803,147 + -1.0 2,803,148 - -0.3 2,803,148 - -1.8
Or see this region's nucleotide sequence