Experiment: KB with Methanol 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1735 and Psyr_1736 are separated by 106 nucleotides Psyr_1736 and Psyr_1737 are separated by 299 nucleotides
Psyr_1735: Psyr_1735 - cysteinyl-tRNA synthetase, at 1,970,482 to 1,971,864
_1735
Psyr_1736: Psyr_1736 - Helix-turn-helix, Fis-type, at 1,971,971 to 1,973,824
_1736
Psyr_1737: Psyr_1737 - carbohydrate ABC transporter ATP-binding protein, CUT1 family, at 1,974,124 to 1,975,218
_1737
Position (kb)
1971
1972
1973
1974 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1971.884 kb on + strand at 1971.884 kb on + strand at 1971.884 kb on + strand at 1971.885 kb on - strand at 1971.888 kb on - strand at 1971.972 kb on + strand at 1971.972 kb on + strand at 1972.002 kb on + strand at 1972.002 kb on + strand at 1972.002 kb on + strand at 1972.002 kb on + strand at 1972.003 kb on - strand at 1972.280 kb on + strand, within Psyr_1736 at 1972.280 kb on + strand, within Psyr_1736 at 1972.281 kb on - strand, within Psyr_1736 at 1972.281 kb on - strand, within Psyr_1736 at 1972.420 kb on - strand, within Psyr_1736 at 1972.490 kb on + strand, within Psyr_1736 at 1972.490 kb on + strand, within Psyr_1736 at 1972.491 kb on - strand, within Psyr_1736 at 1972.492 kb on + strand, within Psyr_1736 at 1972.493 kb on - strand, within Psyr_1736 at 1973.847 kb on + strand at 1973.847 kb on + strand at 1973.847 kb on + strand at 1973.848 kb on - strand at 1973.848 kb on - strand at 1973.862 kb on + strand at 1973.971 kb on + strand at 1974.617 kb on - strand, within Psyr_1737 at 1974.673 kb on - strand, within Psyr_1737 at 1974.682 kb on - strand, within Psyr_1737 at 1974.682 kb on - strand, within Psyr_1737 at 1974.682 kb on - strand, within Psyr_1737 at 1974.685 kb on + strand, within Psyr_1737 at 1974.686 kb on - strand, within Psyr_1737 at 1974.784 kb on + strand, within Psyr_1737
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methanol 5 vol% remove 1,971,884 + -1.7 1,971,884 + -1.1 1,971,884 + +2.6 1,971,885 - -1.4 1,971,888 - -1.4 1,971,972 + -0.4 1,971,972 + -0.8 1,972,002 + -0.1 1,972,002 + +0.2 1,972,002 + +2.2 1,972,002 + -0.8 1,972,003 - +1.2 1,972,280 + Psyr_1736 0.17 -1.4 1,972,280 + Psyr_1736 0.17 -0.8 1,972,281 - Psyr_1736 0.17 +2.6 1,972,281 - Psyr_1736 0.17 +0.2 1,972,420 - Psyr_1736 0.24 -1.1 1,972,490 + Psyr_1736 0.28 -0.8 1,972,490 + Psyr_1736 0.28 -1.0 1,972,491 - Psyr_1736 0.28 -1.1 1,972,492 + Psyr_1736 0.28 -0.2 1,972,493 - Psyr_1736 0.28 -1.2 1,973,847 + +0.2 1,973,847 + -0.7 1,973,847 + +0.4 1,973,848 - -1.6 1,973,848 - +0.9 1,973,862 + -1.5 1,973,971 + +2.2 1,974,617 - Psyr_1737 0.45 -0.4 1,974,673 - Psyr_1737 0.50 +0.1 1,974,682 - Psyr_1737 0.51 -1.7 1,974,682 - Psyr_1737 0.51 +1.3 1,974,682 - Psyr_1737 0.51 -1.5 1,974,685 + Psyr_1737 0.51 +0.2 1,974,686 - Psyr_1737 0.51 -1.9 1,974,784 + Psyr_1737 0.60 -0.1
Or see this region's nucleotide sequence