Experiment: KB with Methanol 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1514 and Psyr_1515 overlap by 4 nucleotides Psyr_1515 and Psyr_1516 overlap by 11 nucleotides
Psyr_1514: Psyr_1514 - type II secretion system protein E, at 1,700,093 to 1,701,724
_1514
Psyr_1515: Psyr_1515 - hypothetical protein, at 1,701,721 to 1,702,323
_1515
Psyr_1516: Psyr_1516 - hypothetical protein, at 1,702,313 to 1,703,671
_1516
Position (kb)
1701
1702
1703 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1700.725 kb on + strand, within Psyr_1514 at 1700.725 kb on + strand, within Psyr_1514 at 1700.812 kb on + strand, within Psyr_1514 at 1700.900 kb on + strand, within Psyr_1514 at 1700.900 kb on + strand, within Psyr_1514 at 1700.900 kb on + strand, within Psyr_1514 at 1700.900 kb on + strand, within Psyr_1514 at 1700.901 kb on - strand, within Psyr_1514 at 1701.000 kb on + strand, within Psyr_1514 at 1701.000 kb on + strand, within Psyr_1514 at 1701.000 kb on + strand, within Psyr_1514 at 1701.000 kb on + strand, within Psyr_1514 at 1701.001 kb on - strand, within Psyr_1514 at 1701.020 kb on + strand, within Psyr_1514 at 1701.020 kb on + strand, within Psyr_1514 at 1701.051 kb on - strand, within Psyr_1514 at 1701.126 kb on + strand, within Psyr_1514 at 1701.127 kb on - strand, within Psyr_1514 at 1701.127 kb on - strand, within Psyr_1514 at 1701.130 kb on - strand, within Psyr_1514 at 1701.130 kb on - strand, within Psyr_1514 at 1701.130 kb on - strand, within Psyr_1514 at 1701.246 kb on + strand, within Psyr_1514 at 1701.246 kb on + strand, within Psyr_1514 at 1701.247 kb on - strand, within Psyr_1514 at 1701.247 kb on - strand, within Psyr_1514 at 1701.247 kb on - strand, within Psyr_1514 at 1701.247 kb on - strand, within Psyr_1514 at 1701.314 kb on - strand, within Psyr_1514 at 1701.314 kb on - strand, within Psyr_1514 at 1701.340 kb on + strand, within Psyr_1514 at 1701.347 kb on + strand, within Psyr_1514 at 1701.347 kb on + strand, within Psyr_1514 at 1701.348 kb on - strand, within Psyr_1514 at 1701.348 kb on - strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.349 kb on + strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.350 kb on - strand, within Psyr_1514 at 1701.454 kb on + strand, within Psyr_1514 at 1701.454 kb on + strand, within Psyr_1514 at 1701.455 kb on - strand, within Psyr_1514 at 1701.455 kb on - strand, within Psyr_1514 at 1701.455 kb on - strand, within Psyr_1514 at 1701.468 kb on + strand, within Psyr_1514 at 1701.468 kb on + strand, within Psyr_1514 at 1701.468 kb on + strand, within Psyr_1514 at 1701.468 kb on + strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.469 kb on - strand, within Psyr_1514 at 1701.534 kb on - strand, within Psyr_1514 at 1701.856 kb on + strand, within Psyr_1515 at 1701.856 kb on + strand, within Psyr_1515 at 1701.857 kb on - strand, within Psyr_1515 at 1701.914 kb on - strand, within Psyr_1515 at 1701.914 kb on - strand, within Psyr_1515 at 1701.914 kb on - strand, within Psyr_1515 at 1701.942 kb on + strand, within Psyr_1515 at 1701.982 kb on + strand, within Psyr_1515 at 1701.993 kb on - strand, within Psyr_1515 at 1701.993 kb on - strand, within Psyr_1515 at 1701.993 kb on - strand, within Psyr_1515 at 1701.993 kb on - strand, within Psyr_1515 at 1702.073 kb on + strand, within Psyr_1515 at 1702.073 kb on + strand, within Psyr_1515 at 1702.086 kb on + strand, within Psyr_1515 at 1702.282 kb on + strand at 1702.282 kb on + strand at 1702.282 kb on + strand at 1702.283 kb on - strand at 1702.283 kb on - strand at 1702.283 kb on - strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.323 kb on + strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.324 kb on - strand at 1702.334 kb on + strand at 1702.335 kb on - strand at 1702.349 kb on + strand at 1702.538 kb on + strand, within Psyr_1516 at 1702.539 kb on - strand, within Psyr_1516 at 1702.696 kb on + strand, within Psyr_1516 at 1702.707 kb on + strand, within Psyr_1516 at 1702.761 kb on + strand, within Psyr_1516 at 1702.761 kb on + strand, within Psyr_1516 at 1702.761 kb on + strand, within Psyr_1516 at 1702.761 kb on + strand, within Psyr_1516 at 1702.761 kb on + strand, within Psyr_1516 at 1702.761 kb on + strand, within Psyr_1516 at 1702.762 kb on - strand, within Psyr_1516 at 1702.762 kb on - strand, within Psyr_1516 at 1702.762 kb on - strand, within Psyr_1516 at 1702.762 kb on - strand, within Psyr_1516 at 1702.762 kb on - strand, within Psyr_1516 at 1702.762 kb on - strand, within Psyr_1516 at 1702.762 kb on - strand, within Psyr_1516 at 1702.806 kb on - strand, within Psyr_1516 at 1702.858 kb on + strand, within Psyr_1516 at 1702.866 kb on - strand, within Psyr_1516 at 1702.898 kb on + strand, within Psyr_1516 at 1702.898 kb on + strand, within Psyr_1516 at 1702.898 kb on + strand, within Psyr_1516 at 1702.898 kb on + strand, within Psyr_1516 at 1702.898 kb on + strand, within Psyr_1516 at 1702.899 kb on - strand, within Psyr_1516 at 1702.899 kb on - strand, within Psyr_1516 at 1702.899 kb on - strand, within Psyr_1516 at 1703.008 kb on + strand, within Psyr_1516 at 1703.008 kb on + strand, within Psyr_1516 at 1703.008 kb on + strand, within Psyr_1516 at 1703.008 kb on + strand, within Psyr_1516 at 1703.008 kb on + strand, within Psyr_1516 at 1703.008 kb on + strand, within Psyr_1516 at 1703.009 kb on - strand, within Psyr_1516 at 1703.009 kb on - strand, within Psyr_1516 at 1703.009 kb on - strand, within Psyr_1516 at 1703.121 kb on + strand, within Psyr_1516 at 1703.255 kb on + strand, within Psyr_1516 at 1703.255 kb on + strand, within Psyr_1516
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methanol 5 vol% remove 1,700,725 + Psyr_1514 0.39 +1.0 1,700,725 + Psyr_1514 0.39 -0.9 1,700,812 + Psyr_1514 0.44 +0.8 1,700,900 + Psyr_1514 0.49 -0.5 1,700,900 + Psyr_1514 0.49 -0.0 1,700,900 + Psyr_1514 0.49 -1.4 1,700,900 + Psyr_1514 0.49 -1.8 1,700,901 - Psyr_1514 0.50 +0.4 1,701,000 + Psyr_1514 0.56 -1.3 1,701,000 + Psyr_1514 0.56 -0.1 1,701,000 + Psyr_1514 0.56 -0.1 1,701,000 + Psyr_1514 0.56 +1.6 1,701,001 - Psyr_1514 0.56 -2.9 1,701,020 + Psyr_1514 0.57 +2.7 1,701,020 + Psyr_1514 0.57 -1.6 1,701,051 - Psyr_1514 0.59 -0.3 1,701,126 + Psyr_1514 0.63 +1.1 1,701,127 - Psyr_1514 0.63 -1.9 1,701,127 - Psyr_1514 0.63 -0.5 1,701,130 - Psyr_1514 0.64 +1.2 1,701,130 - Psyr_1514 0.64 -0.6 1,701,130 - Psyr_1514 0.64 +0.5 1,701,246 + Psyr_1514 0.71 -1.6 1,701,246 + Psyr_1514 0.71 -0.1 1,701,247 - Psyr_1514 0.71 -1.4 1,701,247 - Psyr_1514 0.71 +0.9 1,701,247 - Psyr_1514 0.71 -0.0 1,701,247 - Psyr_1514 0.71 -0.8 1,701,314 - Psyr_1514 0.75 -2.6 1,701,314 - Psyr_1514 0.75 -0.3 1,701,340 + Psyr_1514 0.76 -0.2 1,701,347 + Psyr_1514 0.77 +0.4 1,701,347 + Psyr_1514 0.77 +1.7 1,701,348 - Psyr_1514 0.77 -1.9 1,701,348 - Psyr_1514 0.77 -1.6 1,701,349 + Psyr_1514 0.77 +0.8 1,701,349 + Psyr_1514 0.77 +0.5 1,701,349 + Psyr_1514 0.77 +0.7 1,701,349 + Psyr_1514 0.77 +2.5 1,701,349 + Psyr_1514 0.77 +0.3 1,701,350 - Psyr_1514 0.77 -0.9 1,701,350 - Psyr_1514 0.77 +1.0 1,701,350 - Psyr_1514 0.77 +0.6 1,701,350 - Psyr_1514 0.77 -1.9 1,701,350 - Psyr_1514 0.77 +0.3 1,701,350 - Psyr_1514 0.77 -2.3 1,701,350 - Psyr_1514 0.77 -0.0 1,701,454 + Psyr_1514 0.83 +0.4 1,701,454 + Psyr_1514 0.83 +0.2 1,701,455 - Psyr_1514 0.83 +0.8 1,701,455 - Psyr_1514 0.83 -1.4 1,701,455 - Psyr_1514 0.83 -3.8 1,701,468 + Psyr_1514 0.84 -2.5 1,701,468 + Psyr_1514 0.84 -0.9 1,701,468 + Psyr_1514 0.84 -0.8 1,701,468 + Psyr_1514 0.84 -2.5 1,701,469 - Psyr_1514 0.84 -0.2 1,701,469 - Psyr_1514 0.84 +1.1 1,701,469 - Psyr_1514 0.84 -1.8 1,701,469 - Psyr_1514 0.84 +1.2 1,701,469 - Psyr_1514 0.84 +3.5 1,701,534 - Psyr_1514 0.88 +0.7 1,701,856 + Psyr_1515 0.22 -2.5 1,701,856 + Psyr_1515 0.22 +0.6 1,701,857 - Psyr_1515 0.23 -1.3 1,701,914 - Psyr_1515 0.32 -2.5 1,701,914 - Psyr_1515 0.32 -1.3 1,701,914 - Psyr_1515 0.32 +0.1 1,701,942 + Psyr_1515 0.37 -1.0 1,701,982 + Psyr_1515 0.43 +0.9 1,701,993 - Psyr_1515 0.45 -1.2 1,701,993 - Psyr_1515 0.45 -3.1 1,701,993 - Psyr_1515 0.45 +0.7 1,701,993 - Psyr_1515 0.45 +0.1 1,702,073 + Psyr_1515 0.58 -0.3 1,702,073 + Psyr_1515 0.58 -0.1 1,702,086 + Psyr_1515 0.61 -1.5 1,702,282 + +1.5 1,702,282 + +0.0 1,702,282 + +0.4 1,702,283 - +0.7 1,702,283 - -0.0 1,702,283 - +0.9 1,702,323 + +1.3 1,702,323 + -2.8 1,702,323 + +1.0 1,702,323 + +0.4 1,702,323 + +0.0 1,702,323 + -0.7 1,702,324 - +0.1 1,702,324 - +1.2 1,702,324 - -1.3 1,702,324 - +0.1 1,702,324 - -0.3 1,702,324 - -0.1 1,702,324 - -0.1 1,702,334 + -1.1 1,702,335 - -2.9 1,702,349 + +0.7 1,702,538 + Psyr_1516 0.17 +1.4 1,702,539 - Psyr_1516 0.17 +0.6 1,702,696 + Psyr_1516 0.28 -1.3 1,702,707 + Psyr_1516 0.29 +0.1 1,702,761 + Psyr_1516 0.33 +1.4 1,702,761 + Psyr_1516 0.33 -3.0 1,702,761 + Psyr_1516 0.33 -1.3 1,702,761 + Psyr_1516 0.33 +0.3 1,702,761 + Psyr_1516 0.33 +0.1 1,702,761 + Psyr_1516 0.33 -0.2 1,702,762 - Psyr_1516 0.33 -0.1 1,702,762 - Psyr_1516 0.33 -1.0 1,702,762 - Psyr_1516 0.33 -2.1 1,702,762 - Psyr_1516 0.33 +0.1 1,702,762 - Psyr_1516 0.33 +1.0 1,702,762 - Psyr_1516 0.33 +0.1 1,702,762 - Psyr_1516 0.33 -0.2 1,702,806 - Psyr_1516 0.36 +0.1 1,702,858 + Psyr_1516 0.40 +0.8 1,702,866 - Psyr_1516 0.41 +0.3 1,702,898 + Psyr_1516 0.43 -2.4 1,702,898 + Psyr_1516 0.43 -1.3 1,702,898 + Psyr_1516 0.43 +1.4 1,702,898 + Psyr_1516 0.43 -0.9 1,702,898 + Psyr_1516 0.43 +0.9 1,702,899 - Psyr_1516 0.43 -0.6 1,702,899 - Psyr_1516 0.43 -0.3 1,702,899 - Psyr_1516 0.43 -0.6 1,703,008 + Psyr_1516 0.51 -1.0 1,703,008 + Psyr_1516 0.51 +0.1 1,703,008 + Psyr_1516 0.51 -0.6 1,703,008 + Psyr_1516 0.51 +0.1 1,703,008 + Psyr_1516 0.51 +1.3 1,703,008 + Psyr_1516 0.51 -1.6 1,703,009 - Psyr_1516 0.51 +0.7 1,703,009 - Psyr_1516 0.51 +0.6 1,703,009 - Psyr_1516 0.51 +0.0 1,703,121 + Psyr_1516 0.59 -0.9 1,703,255 + Psyr_1516 0.69 -0.1 1,703,255 + Psyr_1516 0.69 +0.4
Or see this region's nucleotide sequence