Experiment: Varel_Bryant_medium_Glucose with Deoxycholic 0.075 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0474 and BT0475 are separated by 13 nucleotides BT0475 and BT0476 are separated by 4 nucleotides BT0476 and BT0477 are separated by 15 nucleotides BT0477 and BT0478 overlap by 4 nucleotides
BT0474: BT0474 - D-glycero-D-manno-heptose 1-phosphate kinase (NCBI ptt file), at 585,720 to 586,766
BT0474
BT0475: BT0475 - putative phosphoheptose isomerase (NCBI ptt file), at 586,780 to 587,373
BT0475
BT0476: BT0476 - D-mannose-1-phosphate guanyltransferase (NCBI ptt file), at 587,378 to 588,085
BT0476
BT0477: BT0477 - putative phosphatase (NCBI ptt file), at 588,101 to 588,571
BT0477
BT0478: BT0478 - conserved hypothetical protein (NCBI ptt file), at 588,568 to 589,674
BT0478
Position (kb) 587
588
589 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 586.416 kb on - strand, within BT0474 at 586.650 kb on - strand, within BT0474 at 586.673 kb on - strand at 586.710 kb on - strand at 586.771 kb on - strand at 587.038 kb on - strand, within BT0475 at 587.038 kb on - strand, within BT0475 at 587.188 kb on - strand, within BT0475 at 587.323 kb on - strand at 587.439 kb on + strand at 587.488 kb on - strand, within BT0476 at 587.607 kb on - strand, within BT0476 at 587.891 kb on - strand, within BT0476 at 587.893 kb on - strand, within BT0476 at 587.895 kb on - strand, within BT0476 at 587.895 kb on - strand, within BT0476 at 588.148 kb on - strand, within BT0477 at 588.337 kb on - strand, within BT0477 at 588.337 kb on - strand, within BT0477 at 588.446 kb on - strand, within BT0477 at 588.524 kb on - strand, within BT0477 at 588.536 kb on - strand at 588.695 kb on - strand, within BT0478 at 588.811 kb on - strand, within BT0478 at 588.901 kb on - strand, within BT0478 at 588.926 kb on - strand, within BT0478 at 589.005 kb on - strand, within BT0478 at 589.020 kb on + strand, within BT0478 at 589.020 kb on + strand, within BT0478
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Deoxycholic 0.075 mg/ml remove 586,416 - BT0474 0.66 -1.1 586,650 - BT0474 0.89 -0.3 586,673 - -0.1 586,710 - +0.5 586,771 - +0.6 587,038 - BT0475 0.43 -0.4 587,038 - BT0475 0.43 -2.0 587,188 - BT0475 0.69 -1.5 587,323 - -0.7 587,439 + -1.3 587,488 - BT0476 0.16 -0.7 587,607 - BT0476 0.32 -0.3 587,891 - BT0476 0.72 +2.0 587,893 - BT0476 0.73 +2.8 587,895 - BT0476 0.73 +1.2 587,895 - BT0476 0.73 -1.1 588,148 - BT0477 0.10 -1.6 588,337 - BT0477 0.50 -0.5 588,337 - BT0477 0.50 +1.8 588,446 - BT0477 0.73 -1.8 588,524 - BT0477 0.90 -2.2 588,536 - -2.4 588,695 - BT0478 0.11 -1.3 588,811 - BT0478 0.22 +1.1 588,901 - BT0478 0.30 -1.5 588,926 - BT0478 0.32 +0.6 589,005 - BT0478 0.39 -1.5 589,020 + BT0478 0.41 -1.3 589,020 + BT0478 0.41 -0.1
Or see this region's nucleotide sequence