Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21420

Experiment: Disodium Glutarate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21415 and BPHYT_RS21420 are separated by 13 nucleotidesBPHYT_RS21420 and BPHYT_RS21425 are separated by 92 nucleotides BPHYT_RS21415: BPHYT_RS21415 - 3-oxoadipate CoA-transferase subunit A, at 363,604 to 364,311 _RS21415 BPHYT_RS21420: BPHYT_RS21420 - 3-oxoadipate CoA-transferase subunit B, at 364,325 to 364,984 _RS21420 BPHYT_RS21425: BPHYT_RS21425 - 3-carboxy-cis,cis-muconate cycloisomerase, at 365,077 to 366,456 _RS21425 Position (kb) 364 365Strain fitness (log2 ratio) -1 0 1at 363.362 kb on + strandat 363.362 kb on + strandat 363.363 kb on - strandat 363.363 kb on - strandat 363.369 kb on - strandat 363.412 kb on + strandat 363.452 kb on - strandat 363.856 kb on + strand, within BPHYT_RS21415at 363.857 kb on - strand, within BPHYT_RS21415at 363.874 kb on + strand, within BPHYT_RS21415at 363.874 kb on + strand, within BPHYT_RS21415at 364.112 kb on - strand, within BPHYT_RS21415at 364.388 kb on + strandat 364.414 kb on + strand, within BPHYT_RS21420at 364.571 kb on + strand, within BPHYT_RS21420at 364.748 kb on + strand, within BPHYT_RS21420at 364.844 kb on + strand, within BPHYT_RS21420at 364.845 kb on - strand, within BPHYT_RS21420at 365.038 kb on + strandat 365.038 kb on + strandat 365.381 kb on - strand, within BPHYT_RS21425

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Per-strain Table

Position Strand Gene LocusTag Fraction Disodium Glutarate (C)
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363,362 + +1.7
363,362 + +1.1
363,363 - -0.3
363,363 - -0.6
363,369 - +0.7
363,412 + -1.1
363,452 - -0.0
363,856 + BPHYT_RS21415 0.36 +0.6
363,857 - BPHYT_RS21415 0.36 -0.7
363,874 + BPHYT_RS21415 0.38 +1.0
363,874 + BPHYT_RS21415 0.38 +1.2
364,112 - BPHYT_RS21415 0.72 +1.1
364,388 + -0.4
364,414 + BPHYT_RS21420 0.13 +0.2
364,571 + BPHYT_RS21420 0.37 -0.1
364,748 + BPHYT_RS21420 0.64 -0.2
364,844 + BPHYT_RS21420 0.79 -0.2
364,845 - BPHYT_RS21420 0.79 -0.4
365,038 + +0.0
365,038 + -0.1
365,381 - BPHYT_RS21425 0.22 -0.1

Or see this region's nucleotide sequence