Experiment: Varel_Bryant_medium_Glucose with Deoxycholic 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2798 and BT2799 are separated by 44 nucleotides BT2799 and tRNA-Asn are separated by 77 nucleotides tRNA-Asn and tRNA-Asn are separated by 24 nucleotides tRNA-Asn and BT2800 are separated by 421 nucleotides
BT2798: BT2798 - carboxy-terminal processing protease precursor (NCBI ptt file), at 3,471,884 to 3,473,581
BT2798
BT2799: BT2799 - putative PfkB family carbohydrate kinase (NCBI ptt file), at 3,473,626 to 3,474,615
BT2799
BT_t50: tRNA-Asn - tRNA-Asn (RefSeq), at 3,474,693 to 3,474,769
tRNA-Asn
BT_t51: tRNA-Asn - tRNA-Asn (RefSeq), at 3,474,794 to 3,474,870
tRNA-Asn
BT2800: BT2800 - putative amidophosphoribosyl-transferase (NCBI ptt file), at 3,475,292 to 3,475,999
BT2800
Position (kb)
3473
3474
3475 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3472.691 kb on - strand, within BT2798 at 3472.706 kb on + strand, within BT2798 at 3472.752 kb on + strand, within BT2798 at 3472.796 kb on + strand, within BT2798 at 3472.819 kb on - strand, within BT2798 at 3472.888 kb on + strand, within BT2798 at 3472.889 kb on - strand, within BT2798 at 3472.966 kb on + strand, within BT2798 at 3472.967 kb on - strand, within BT2798 at 3472.968 kb on + strand, within BT2798 at 3472.978 kb on + strand, within BT2798 at 3472.978 kb on + strand, within BT2798 at 3472.981 kb on + strand, within BT2798 at 3472.982 kb on - strand, within BT2798 at 3472.999 kb on - strand, within BT2798 at 3472.999 kb on - strand, within BT2798 at 3473.021 kb on - strand, within BT2798 at 3473.030 kb on + strand, within BT2798 at 3473.035 kb on + strand, within BT2798 at 3473.035 kb on + strand, within BT2798 at 3473.036 kb on - strand, within BT2798 at 3473.071 kb on + strand, within BT2798 at 3473.183 kb on - strand, within BT2798 at 3473.261 kb on - strand, within BT2798 at 3473.263 kb on + strand, within BT2798 at 3473.265 kb on + strand, within BT2798 at 3473.266 kb on - strand, within BT2798 at 3473.266 kb on - strand, within BT2798 at 3473.270 kb on - strand, within BT2798 at 3473.270 kb on - strand, within BT2798 at 3473.270 kb on - strand, within BT2798 at 3473.285 kb on - strand, within BT2798 at 3473.343 kb on + strand, within BT2798 at 3473.343 kb on + strand, within BT2798 at 3473.343 kb on + strand, within BT2798 at 3473.344 kb on - strand, within BT2798 at 3473.350 kb on + strand, within BT2798 at 3473.350 kb on + strand, within BT2798 at 3473.374 kb on + strand, within BT2798 at 3473.374 kb on + strand, within BT2798 at 3473.375 kb on - strand, within BT2798 at 3473.424 kb on + strand at 3473.425 kb on - strand at 3473.456 kb on - strand at 3473.528 kb on - strand at 3473.528 kb on - strand at 3473.528 kb on - strand at 3473.534 kb on + strand at 3473.535 kb on - strand at 3473.556 kb on - strand at 3473.628 kb on - strand at 3473.654 kb on + strand at 3473.863 kb on - strand, within BT2799 at 3473.874 kb on + strand, within BT2799 at 3473.877 kb on - strand, within BT2799 at 3473.904 kb on + strand, within BT2799 at 3474.005 kb on + strand, within BT2799 at 3474.009 kb on + strand at 3474.150 kb on - strand, within BT2799 at 3474.151 kb on + strand, within BT2799 at 3474.161 kb on + strand, within BT2799 at 3474.161 kb on + strand, within BT2799 at 3474.162 kb on - strand, within BT2799 at 3474.167 kb on + strand, within BT2799 at 3474.227 kb on + strand, within BT2799 at 3474.241 kb on - strand, within BT2799 at 3474.529 kb on + strand at 3474.530 kb on - strand at 3474.574 kb on + strand at 3474.574 kb on + strand at 3475.059 kb on - strand at 3475.063 kb on + strand at 3475.076 kb on + strand at 3475.100 kb on + strand at 3475.100 kb on + strand at 3475.122 kb on + strand at 3475.142 kb on + strand at 3475.179 kb on + strand at 3475.198 kb on - strand at 3475.201 kb on + strand at 3475.201 kb on + strand at 3475.201 kb on + strand at 3475.201 kb on + strand at 3475.202 kb on - strand at 3475.224 kb on - strand at 3475.224 kb on - strand at 3475.224 kb on - strand at 3475.228 kb on + strand at 3475.229 kb on - strand at 3475.229 kb on - strand at 3475.229 kb on - strand at 3475.246 kb on - strand at 3475.252 kb on + strand at 3475.253 kb on - strand at 3475.257 kb on - strand at 3475.364 kb on + strand, within BT2800 at 3475.369 kb on + strand, within BT2800 at 3475.406 kb on + strand, within BT2800 at 3475.406 kb on + strand, within BT2800 at 3475.406 kb on + strand, within BT2800 at 3475.407 kb on - strand, within BT2800 at 3475.407 kb on - strand, within BT2800 at 3475.475 kb on + strand, within BT2800 at 3475.517 kb on + strand, within BT2800 at 3475.518 kb on - strand, within BT2800 at 3475.518 kb on - strand at 3475.550 kb on + strand, within BT2800 at 3475.590 kb on + strand, within BT2800 at 3475.599 kb on - strand, within BT2800
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Deoxycholic 0.2 mg/ml remove 3,472,691 - BT2798 0.48 -2.6 3,472,706 + BT2798 0.48 +0.4 3,472,752 + BT2798 0.51 -1.5 3,472,796 + BT2798 0.54 -1.4 3,472,819 - BT2798 0.55 -2.9 3,472,888 + BT2798 0.59 -2.5 3,472,889 - BT2798 0.59 -0.7 3,472,966 + BT2798 0.64 -2.8 3,472,967 - BT2798 0.64 -2.0 3,472,968 + BT2798 0.64 -1.3 3,472,978 + BT2798 0.64 -3.6 3,472,978 + BT2798 0.64 -1.0 3,472,981 + BT2798 0.65 -1.2 3,472,982 - BT2798 0.65 +0.8 3,472,999 - BT2798 0.66 +0.4 3,472,999 - BT2798 0.66 -1.3 3,473,021 - BT2798 0.67 -3.4 3,473,030 + BT2798 0.67 -2.1 3,473,035 + BT2798 0.68 -2.0 3,473,035 + BT2798 0.68 -2.8 3,473,036 - BT2798 0.68 -2.2 3,473,071 + BT2798 0.70 +0.0 3,473,183 - BT2798 0.77 -1.3 3,473,261 - BT2798 0.81 -2.6 3,473,263 + BT2798 0.81 -0.1 3,473,265 + BT2798 0.81 -0.2 3,473,266 - BT2798 0.81 -1.2 3,473,266 - BT2798 0.81 -2.7 3,473,270 - BT2798 0.82 -0.6 3,473,270 - BT2798 0.82 -2.6 3,473,270 - BT2798 0.82 -2.2 3,473,285 - BT2798 0.83 -1.3 3,473,343 + BT2798 0.86 -1.6 3,473,343 + BT2798 0.86 -1.2 3,473,343 + BT2798 0.86 -1.0 3,473,344 - BT2798 0.86 -1.8 3,473,350 + BT2798 0.86 -1.6 3,473,350 + BT2798 0.86 -1.3 3,473,374 + BT2798 0.88 -0.6 3,473,374 + BT2798 0.88 -2.5 3,473,375 - BT2798 0.88 -1.6 3,473,424 + -1.5 3,473,425 - -0.1 3,473,456 - -2.4 3,473,528 - -2.7 3,473,528 - +0.6 3,473,528 - -1.1 3,473,534 + -1.8 3,473,535 - +0.2 3,473,556 - +0.4 3,473,628 - +1.6 3,473,654 + -1.2 3,473,863 - BT2799 0.24 +0.2 3,473,874 + BT2799 0.25 -0.3 3,473,877 - BT2799 0.25 -1.3 3,473,904 + BT2799 0.28 +0.9 3,474,005 + BT2799 0.38 +0.6 3,474,009 + -0.7 3,474,150 - BT2799 0.53 +0.5 3,474,151 + BT2799 0.53 +0.7 3,474,161 + BT2799 0.54 -0.2 3,474,161 + BT2799 0.54 -1.1 3,474,162 - BT2799 0.54 -0.2 3,474,167 + BT2799 0.55 +0.5 3,474,227 + BT2799 0.61 +0.1 3,474,241 - BT2799 0.62 -0.3 3,474,529 + -6.1 3,474,530 - -0.3 3,474,574 + +0.0 3,474,574 + -0.6 3,475,059 - -0.8 3,475,063 + -0.6 3,475,076 + -1.9 3,475,100 + -0.9 3,475,100 + -0.2 3,475,122 + -2.0 3,475,142 + -0.7 3,475,179 + -1.1 3,475,198 - +0.8 3,475,201 + +0.1 3,475,201 + +0.3 3,475,201 + -0.9 3,475,201 + -1.1 3,475,202 - +0.0 3,475,224 - -0.8 3,475,224 - -1.6 3,475,224 - -0.2 3,475,228 + -0.6 3,475,229 - -1.6 3,475,229 - -0.7 3,475,229 - +0.0 3,475,246 - +1.7 3,475,252 + -0.5 3,475,253 - -1.0 3,475,257 - +0.8 3,475,364 + BT2800 0.10 +0.0 3,475,369 + BT2800 0.11 +0.4 3,475,406 + BT2800 0.16 -0.2 3,475,406 + BT2800 0.16 -0.6 3,475,406 + BT2800 0.16 -0.1 3,475,407 - BT2800 0.16 -1.1 3,475,407 - BT2800 0.16 +0.7 3,475,475 + BT2800 0.26 -0.2 3,475,517 + BT2800 0.32 -1.4 3,475,518 - BT2800 0.32 -1.0 3,475,518 - -2.0 3,475,550 + BT2800 0.36 -0.3 3,475,590 + BT2800 0.42 -1.6 3,475,599 - BT2800 0.43 -0.5
Or see this region's nucleotide sequence