Experiment: Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3249 and BT3250 are separated by 2 nucleotides BT3250 and BT3251 are separated by 46 nucleotides BT3251 and BT3252 overlap by 16 nucleotides BT3252 and BT3253 overlap by 1 nucleotides
BT3249: BT3249 - putative zinc ABC transporter, zinc-binding protein (NCBI ptt file), at 4,148,994 to 4,149,920
BT3249
BT3250: BT3250 - conserved hypothetical protein (NCBI ptt file), at 4,149,923 to 4,150,645
BT3250
BT3251: BT3251 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 4,150,692 to 4,151,171
BT3251
BT3252: BT3252 - hypothetical protein (NCBI ptt file), at 4,151,156 to 4,152,166
BT3252
BT3253: BT3253 - phosphoribosylamine--glycine ligase (NCBI ptt file), at 4,152,166 to 4,153,440
BT3253
Position (kb)
4150
4151
4152 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4149.700 kb on + strand, within BT3249 at 4149.700 kb on + strand, within BT3249 at 4149.701 kb on - strand, within BT3249 at 4149.701 kb on - strand, within BT3249 at 4149.924 kb on + strand at 4149.929 kb on + strand at 4149.930 kb on - strand at 4149.930 kb on - strand at 4149.971 kb on + strand at 4149.972 kb on - strand at 4149.972 kb on - strand at 4150.066 kb on - strand, within BT3250 at 4150.066 kb on - strand, within BT3250 at 4150.066 kb on - strand, within BT3250 at 4150.066 kb on - strand at 4150.095 kb on - strand, within BT3250 at 4150.102 kb on - strand, within BT3250 at 4150.168 kb on - strand, within BT3250 at 4150.254 kb on - strand, within BT3250 at 4150.280 kb on - strand, within BT3250 at 4150.286 kb on + strand, within BT3250 at 4150.309 kb on + strand, within BT3250 at 4150.350 kb on - strand, within BT3250 at 4150.350 kb on - strand, within BT3250 at 4150.350 kb on - strand, within BT3250 at 4150.350 kb on - strand, within BT3250 at 4150.351 kb on + strand, within BT3250 at 4150.353 kb on + strand, within BT3250 at 4150.406 kb on - strand, within BT3250 at 4150.406 kb on - strand, within BT3250 at 4150.408 kb on - strand, within BT3250 at 4150.412 kb on + strand, within BT3250 at 4150.413 kb on - strand, within BT3250 at 4150.413 kb on - strand, within BT3250 at 4150.418 kb on - strand, within BT3250 at 4150.418 kb on - strand, within BT3250 at 4150.418 kb on - strand, within BT3250 at 4150.418 kb on - strand, within BT3250 at 4150.448 kb on - strand, within BT3250 at 4150.537 kb on + strand, within BT3250 at 4150.538 kb on - strand, within BT3250 at 4150.665 kb on - strand at 4150.665 kb on - strand at 4150.667 kb on + strand at 4150.675 kb on - strand at 4150.679 kb on - strand at 4150.689 kb on - strand at 4150.725 kb on + strand at 4150.725 kb on + strand at 4150.730 kb on - strand at 4150.730 kb on - strand at 4150.744 kb on + strand, within BT3251 at 4150.744 kb on + strand, within BT3251 at 4150.745 kb on - strand, within BT3251 at 4150.745 kb on - strand, within BT3251 at 4150.750 kb on - strand, within BT3251 at 4150.791 kb on - strand, within BT3251 at 4150.791 kb on - strand, within BT3251 at 4150.794 kb on - strand, within BT3251 at 4150.966 kb on + strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.967 kb on - strand, within BT3251 at 4150.970 kb on - strand, within BT3251 at 4150.970 kb on - strand, within BT3251 at 4151.016 kb on - strand, within BT3251 at 4151.053 kb on - strand at 4151.084 kb on + strand, within BT3251 at 4151.085 kb on - strand, within BT3251 at 4151.098 kb on - strand, within BT3251 at 4151.146 kb on - strand at 4152.170 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml remove 4,149,700 + BT3249 0.76 +0.4 4,149,700 + BT3249 0.76 -1.8 4,149,701 - BT3249 0.76 -0.1 4,149,701 - BT3249 0.76 -2.8 4,149,924 + -0.9 4,149,929 + -0.8 4,149,930 - +0.9 4,149,930 - -0.1 4,149,971 + +0.0 4,149,972 - +1.0 4,149,972 - -0.1 4,150,066 - BT3250 0.20 +1.0 4,150,066 - BT3250 0.20 +0.4 4,150,066 - BT3250 0.20 +1.7 4,150,066 - +1.7 4,150,095 - BT3250 0.24 +1.7 4,150,102 - BT3250 0.25 +0.4 4,150,168 - BT3250 0.34 +0.1 4,150,254 - BT3250 0.46 -3.7 4,150,280 - BT3250 0.49 -0.6 4,150,286 + BT3250 0.50 -1.1 4,150,309 + BT3250 0.53 -1.2 4,150,350 - BT3250 0.59 -0.5 4,150,350 - BT3250 0.59 +0.5 4,150,350 - BT3250 0.59 -0.9 4,150,350 - BT3250 0.59 +0.4 4,150,351 + BT3250 0.59 -0.6 4,150,353 + BT3250 0.59 -0.5 4,150,406 - BT3250 0.67 +0.2 4,150,406 - BT3250 0.67 -1.0 4,150,408 - BT3250 0.67 +0.1 4,150,412 + BT3250 0.68 -1.8 4,150,413 - BT3250 0.68 +0.9 4,150,413 - BT3250 0.68 -1.2 4,150,418 - BT3250 0.68 -0.9 4,150,418 - BT3250 0.68 -1.1 4,150,418 - BT3250 0.68 -1.3 4,150,418 - BT3250 0.68 -1.4 4,150,448 - BT3250 0.73 -0.1 4,150,537 + BT3250 0.85 -1.9 4,150,538 - BT3250 0.85 -0.3 4,150,665 - +2.4 4,150,665 - +0.1 4,150,667 + -1.1 4,150,675 - +0.1 4,150,679 - -0.1 4,150,689 - +0.1 4,150,725 + +0.5 4,150,725 + -1.1 4,150,730 - +1.4 4,150,730 - +1.6 4,150,744 + BT3251 0.11 +0.7 4,150,744 + BT3251 0.11 -1.1 4,150,745 - BT3251 0.11 +1.4 4,150,745 - BT3251 0.11 +1.0 4,150,750 - BT3251 0.12 +2.3 4,150,791 - BT3251 0.21 +1.8 4,150,791 - BT3251 0.21 +1.1 4,150,794 - BT3251 0.21 +1.9 4,150,966 + BT3251 0.57 -1.1 4,150,967 - BT3251 0.57 +0.7 4,150,967 - BT3251 0.57 +0.1 4,150,967 - BT3251 0.57 -0.1 4,150,967 - BT3251 0.57 +1.7 4,150,967 - BT3251 0.57 +1.4 4,150,970 - BT3251 0.58 +1.5 4,150,970 - BT3251 0.58 +0.5 4,151,016 - BT3251 0.68 +0.7 4,151,053 - -1.1 4,151,084 + BT3251 0.82 +0.7 4,151,085 - BT3251 0.82 +1.3 4,151,098 - BT3251 0.85 -0.5 4,151,146 - +1.3 4,152,170 - -2.4
Or see this region's nucleotide sequence