Experiment: Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT_2008 and BT2009 are separated by 28 nucleotides BT2009 and BT2010 overlap by 4 nucleotides BT2010 and BT2011 are separated by 95 nucleotides BT2011 and BT2012 overlap by 38 nucleotides
BT_2008: BT_2008 - hypothetical protein (RefSeq), at 2,524,219 to 2,525,097
_2008
BT2009: BT2009 - guanylate kinase (GMP kinase) (NCBI ptt file), at 2,525,126 to 2,525,740
BT2009
BT2010: BT2010 - hypothetical protein (NCBI ptt file), at 2,525,737 to 2,525,991
BT2010
BT2011: BT2011 - putative nicotinate-nucleotide adenylyltransferase (NCBI ptt file), at 2,526,087 to 2,526,695
BT2011
BT2012: BT2012 - conserved hypothetical protein, putative ATPase (NCBI ptt file), at 2,526,658 to 2,527,947
BT2012
Position (kb)
2526
2527 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2525.831 kb on + strand, within BT2010 at 2525.835 kb on + strand, within BT2010 at 2526.041 kb on + strand at 2526.659 kb on + strand at 2526.659 kb on + strand at 2526.660 kb on - strand at 2526.668 kb on + strand at 2526.669 kb on - strand at 2526.693 kb on + strand at 2526.693 kb on + strand at 2526.694 kb on - strand at 2526.736 kb on + strand at 2526.871 kb on - strand, within BT2012 at 2526.891 kb on + strand, within BT2012 at 2526.922 kb on + strand, within BT2012 at 2526.923 kb on - strand at 2526.925 kb on + strand, within BT2012 at 2526.940 kb on - strand, within BT2012 at 2526.940 kb on - strand, within BT2012 at 2526.951 kb on + strand, within BT2012 at 2526.952 kb on - strand, within BT2012 at 2527.008 kb on + strand, within BT2012 at 2527.009 kb on - strand, within BT2012 at 2527.009 kb on - strand, within BT2012 at 2527.045 kb on - strand, within BT2012 at 2527.109 kb on + strand at 2527.109 kb on + strand, within BT2012 at 2527.114 kb on - strand, within BT2012 at 2527.192 kb on - strand, within BT2012 at 2527.296 kb on - strand, within BT2012 at 2527.296 kb on - strand, within BT2012 at 2527.350 kb on + strand, within BT2012 at 2527.388 kb on + strand, within BT2012 at 2527.388 kb on + strand, within BT2012 at 2527.463 kb on - strand, within BT2012 at 2527.522 kb on + strand, within BT2012 at 2527.566 kb on + strand, within BT2012 at 2527.567 kb on - strand, within BT2012 at 2527.634 kb on + strand, within BT2012 at 2527.687 kb on + strand, within BT2012
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml remove 2,525,831 + BT2010 0.37 -0.1 2,525,835 + BT2010 0.38 -1.5 2,526,041 + +0.6 2,526,659 + -0.2 2,526,659 + -0.1 2,526,660 - -0.6 2,526,668 + -0.0 2,526,669 - -1.9 2,526,693 + -0.5 2,526,693 + +0.9 2,526,694 - +3.9 2,526,736 + +0.4 2,526,871 - BT2012 0.17 +2.6 2,526,891 + BT2012 0.18 +0.1 2,526,922 + BT2012 0.20 -0.3 2,526,923 - +0.4 2,526,925 + BT2012 0.21 -0.7 2,526,940 - BT2012 0.22 -1.4 2,526,940 - BT2012 0.22 -0.8 2,526,951 + BT2012 0.23 -2.0 2,526,952 - BT2012 0.23 +1.6 2,527,008 + BT2012 0.27 -1.2 2,527,009 - BT2012 0.27 +0.1 2,527,009 - BT2012 0.27 +0.8 2,527,045 - BT2012 0.30 -0.7 2,527,109 + +0.6 2,527,109 + BT2012 0.35 -3.3 2,527,114 - BT2012 0.35 -0.6 2,527,192 - BT2012 0.41 -0.7 2,527,296 - BT2012 0.49 +0.1 2,527,296 - BT2012 0.49 -1.6 2,527,350 + BT2012 0.54 -2.3 2,527,388 + BT2012 0.57 -0.5 2,527,388 + BT2012 0.57 -1.3 2,527,463 - BT2012 0.62 -1.2 2,527,522 + BT2012 0.67 -0.4 2,527,566 + BT2012 0.70 -0.4 2,527,567 - BT2012 0.70 -0.2 2,527,634 + BT2012 0.76 -0.4 2,527,687 + BT2012 0.80 -0.9
Or see this region's nucleotide sequence