Experiment: Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2007 and BT_2008 are separated by 89 nucleotides BT_2008 and BT2009 are separated by 28 nucleotides BT2009 and BT2010 overlap by 4 nucleotides BT2010 and BT2011 are separated by 95 nucleotides BT2011 and BT2012 overlap by 38 nucleotides
BT2007: BT2007 - conserved hypothetical protein, putative glycoprotease (NCBI ptt file), at 2,523,434 to 2,524,129
BT2007
BT_2008: BT_2008 - hypothetical protein (RefSeq), at 2,524,219 to 2,525,097
_2008
BT2009: BT2009 - guanylate kinase (GMP kinase) (NCBI ptt file), at 2,525,126 to 2,525,740
BT2009
BT2010: BT2010 - hypothetical protein (NCBI ptt file), at 2,525,737 to 2,525,991
BT2010
BT2011: BT2011 - putative nicotinate-nucleotide adenylyltransferase (NCBI ptt file), at 2,526,087 to 2,526,695
BT2011
BT2012: BT2012 - conserved hypothetical protein, putative ATPase (NCBI ptt file), at 2,526,658 to 2,527,947
BT2012
Position (kb)
2525
2526 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2524.145 kb on - strand at 2524.283 kb on + strand at 2524.713 kb on + strand, within BT_2008 at 2524.724 kb on + strand, within BT_2008 at 2524.819 kb on + strand, within BT_2008 at 2525.831 kb on + strand, within BT2010 at 2525.835 kb on + strand, within BT2010 at 2526.041 kb on + strand at 2526.659 kb on + strand at 2526.659 kb on + strand at 2526.660 kb on - strand at 2526.668 kb on + strand at 2526.669 kb on - strand at 2526.693 kb on + strand at 2526.693 kb on + strand at 2526.694 kb on - strand at 2526.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml remove 2,524,145 - -2.8 2,524,283 + -3.4 2,524,713 + BT_2008 0.56 +0.6 2,524,724 + BT_2008 0.57 -3.9 2,524,819 + BT_2008 0.68 -1.9 2,525,831 + BT2010 0.37 -0.1 2,525,835 + BT2010 0.38 -1.5 2,526,041 + +0.6 2,526,659 + -0.2 2,526,659 + -0.1 2,526,660 - -0.6 2,526,668 + -0.0 2,526,669 - -1.9 2,526,693 + -0.5 2,526,693 + +0.9 2,526,694 - +3.9 2,526,736 + +0.4
Or see this region's nucleotide sequence