Experiment: Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0201 and BT0202 overlap by 4 nucleotides BT0202 and BT0203 are separated by 3 nucleotides
BT0201: BT0201 - histidinol dehydrogenase (NCBI ptt file), at 215,955 to 217,241
BT0201
BT0202: BT0202 - histidinol-phosphate aminotransferase (EC 2.6.1.9) <small>(from data)</small>, at 217,238 to 218,278
BT0202
BT0203: BT0203 - histidine biosynthesis bifunctional protein hisB (NCBI ptt file), at 218,282 to 219,406
BT0203
Position (kb) 217
218
219 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 216.309 kb on - strand, within BT0201 at 216.330 kb on - strand, within BT0201 at 216.455 kb on + strand, within BT0201 at 216.455 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.494 kb on - strand, within BT0201 at 216.534 kb on + strand, within BT0201 at 216.535 kb on - strand, within BT0201 at 216.629 kb on - strand, within BT0201 at 216.713 kb on - strand, within BT0201 at 216.787 kb on + strand, within BT0201 at 216.799 kb on + strand, within BT0201 at 216.800 kb on - strand, within BT0201 at 217.046 kb on + strand, within BT0201 at 217.047 kb on - strand, within BT0201 at 217.047 kb on - strand, within BT0201 at 217.230 kb on - strand at 217.271 kb on - strand at 217.282 kb on - strand at 217.311 kb on - strand at 217.313 kb on - strand at 217.361 kb on - strand, within BT0202 at 217.447 kb on + strand, within BT0202 at 217.448 kb on - strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.524 kb on + strand, within BT0202 at 217.525 kb on - strand, within BT0202 at 217.545 kb on - strand, within BT0202 at 217.554 kb on - strand, within BT0202 at 217.658 kb on - strand, within BT0202 at 217.731 kb on + strand, within BT0202 at 217.773 kb on - strand, within BT0202 at 217.775 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.941 kb on - strand, within BT0202 at 217.992 kb on - strand, within BT0202 at 217.995 kb on - strand, within BT0202 at 218.084 kb on + strand, within BT0202 at 218.085 kb on - strand, within BT0202 at 218.277 kb on - strand at 218.281 kb on + strand at 218.281 kb on + strand at 218.282 kb on - strand at 218.309 kb on - strand at 218.346 kb on - strand at 218.627 kb on - strand, within BT0203 at 218.627 kb on - strand, within BT0203 at 218.870 kb on - strand, within BT0203 at 218.873 kb on + strand, within BT0203 at 218.874 kb on - strand, within BT0203 at 218.874 kb on - strand, within BT0203 at 219.058 kb on - strand, within BT0203 at 219.116 kb on - strand, within BT0203 at 219.172 kb on - strand, within BT0203
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml remove 216,309 - BT0201 0.28 -2.3 216,330 - BT0201 0.29 -3.9 216,455 + BT0201 0.39 -4.6 216,455 - BT0201 0.39 -1.9 216,494 - BT0201 0.42 -3.7 216,494 - BT0201 0.42 -2.5 216,494 - BT0201 0.42 -1.3 216,534 + BT0201 0.45 -2.4 216,535 - BT0201 0.45 -0.4 216,629 - BT0201 0.52 -2.0 216,713 - BT0201 0.59 -0.4 216,787 + BT0201 0.65 -3.0 216,799 + BT0201 0.66 -1.8 216,800 - BT0201 0.66 -2.4 217,046 + BT0201 0.85 -0.8 217,047 - BT0201 0.85 -1.6 217,047 - BT0201 0.85 -2.4 217,230 - -1.0 217,271 - -4.2 217,282 - -3.7 217,311 - -3.1 217,313 - -1.8 217,361 - BT0202 0.12 -2.0 217,447 + BT0202 0.20 -2.0 217,448 - BT0202 0.20 -0.8 217,524 + BT0202 0.27 -2.2 217,524 + BT0202 0.27 -0.5 217,524 + BT0202 0.27 -4.0 217,525 - BT0202 0.28 -2.8 217,545 - BT0202 0.29 -0.0 217,554 - BT0202 0.30 -4.4 217,658 - BT0202 0.40 -3.5 217,731 + BT0202 0.47 -2.2 217,773 - BT0202 0.51 -3.1 217,775 - BT0202 0.52 -2.8 217,941 - BT0202 0.68 -3.1 217,941 - BT0202 0.68 -5.5 217,941 - BT0202 0.68 -4.0 217,992 - BT0202 0.72 -4.4 217,995 - BT0202 0.73 -1.0 218,084 + BT0202 0.81 -3.8 218,085 - BT0202 0.81 -3.6 218,277 - -2.9 218,281 + +0.6 218,281 + -1.3 218,282 - -3.2 218,309 - -5.3 218,346 - -7.5 218,627 - BT0203 0.31 -5.0 218,627 - BT0203 0.31 -1.4 218,870 - BT0203 0.52 -3.7 218,873 + BT0203 0.53 -1.3 218,874 - BT0203 0.53 -2.3 218,874 - BT0203 0.53 -1.6 219,058 - BT0203 0.69 -1.0 219,116 - BT0203 0.74 -0.4 219,172 - BT0203 0.79 -2.1
Or see this region's nucleotide sequence