Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23925

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23920 and BWI76_RS23925 are separated by 269 nucleotidesBWI76_RS23925 and BWI76_RS23930 are separated by 169 nucleotides BWI76_RS23920: BWI76_RS23920 - D-3-phosphoglycerate dehydrogenase, at 4,991,524 to 4,992,756 _RS23920 BWI76_RS23925: BWI76_RS23925 - ribose-5-phosphate isomerase, at 4,993,026 to 4,993,685 _RS23925 BWI76_RS23930: BWI76_RS23930 - transcriptional regulator ArgP, at 4,993,855 to 4,994,748 _RS23930 Position (kb) 4993 4994Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 4992.264 kb on - strand, within BWI76_RS23920at 4992.658 kb on + strandat 4992.671 kb on + strandat 4992.746 kb on + strandat 4992.746 kb on + strandat 4992.802 kb on - strandat 4993.048 kb on + strandat 4993.057 kb on + strandat 4993.407 kb on + strand, within BWI76_RS23925at 4993.628 kb on + strandat 4993.845 kb on + strandat 4993.877 kb on - strandat 4993.885 kb on - strandat 4993.904 kb on + strandat 4994.015 kb on + strand, within BWI76_RS23930at 4994.269 kb on + strand, within BWI76_RS23930at 4994.288 kb on + strand, within BWI76_RS23930at 4994.357 kb on + strand, within BWI76_RS23930at 4994.402 kb on + strand, within BWI76_RS23930

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL
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4,992,264 - BWI76_RS23920 0.60 -2.6
4,992,658 + -3.1
4,992,671 + -5.7
4,992,746 + -3.6
4,992,746 + -1.9
4,992,802 - +2.4
4,993,048 + -1.4
4,993,057 + -3.5
4,993,407 + BWI76_RS23925 0.58 -1.2
4,993,628 + -2.0
4,993,845 + -0.7
4,993,877 - -0.4
4,993,885 - -1.3
4,993,904 + -0.8
4,994,015 + BWI76_RS23930 0.18 -1.7
4,994,269 + BWI76_RS23930 0.46 -1.5
4,994,288 + BWI76_RS23930 0.48 -1.4
4,994,357 + BWI76_RS23930 0.56 -1.4
4,994,402 + BWI76_RS23930 0.61 -1.1

Or see this region's nucleotide sequence