Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16970 and BWI76_RS16975 are separated by 101 nucleotides BWI76_RS16975 and BWI76_RS16980 are separated by 11 nucleotides BWI76_RS16980 and BWI76_RS16985 overlap by 4 nucleotides
BWI76_RS16970: BWI76_RS16970 - murein L,D-transpeptidase, at 3,519,587 to 3,520,570
_RS16970
BWI76_RS16975: BWI76_RS16975 - cysteine desufuration protein SufE, at 3,520,672 to 3,521,088
_RS16975
BWI76_RS16980: BWI76_RS16980 - bifunctional cysteine desulfurase/selenocysteine lyase, at 3,521,100 to 3,522,320
_RS16980
BWI76_RS16985: BWI76_RS16985 - signal peptide peptidase SppA, at 3,522,317 to 3,523,591
_RS16985
Position (kb)
3521
3522
3523 Strain fitness (log2 ratio)
-2
-1
0
1 at 3520.206 kb on + strand, within BWI76_RS16970 at 3520.272 kb on - strand, within BWI76_RS16970 at 3520.441 kb on - strand, within BWI76_RS16970 at 3520.443 kb on + strand, within BWI76_RS16970 at 3520.550 kb on + strand at 3520.648 kb on - strand at 3520.953 kb on + strand, within BWI76_RS16975 at 3521.043 kb on + strand, within BWI76_RS16975 at 3521.051 kb on - strand at 3521.158 kb on + strand at 3521.161 kb on + strand at 3521.169 kb on - strand at 3521.211 kb on - strand at 3521.367 kb on + strand, within BWI76_RS16980 at 3521.390 kb on + strand, within BWI76_RS16980 at 3521.708 kb on - strand, within BWI76_RS16980 at 3522.043 kb on - strand, within BWI76_RS16980 at 3522.327 kb on + strand at 3522.647 kb on + strand, within BWI76_RS16985
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL remove 3,520,206 + BWI76_RS16970 0.63 -0.1 3,520,272 - BWI76_RS16970 0.70 +0.1 3,520,441 - BWI76_RS16970 0.87 -0.1 3,520,443 + BWI76_RS16970 0.87 -0.7 3,520,550 + +0.8 3,520,648 - -0.7 3,520,953 + BWI76_RS16975 0.67 -1.2 3,521,043 + BWI76_RS16975 0.89 -1.0 3,521,051 - +0.3 3,521,158 + -1.6 3,521,161 + -0.6 3,521,169 - -0.7 3,521,211 - -0.9 3,521,367 + BWI76_RS16980 0.22 -0.8 3,521,390 + BWI76_RS16980 0.24 -0.8 3,521,708 - BWI76_RS16980 0.50 -2.5 3,522,043 - BWI76_RS16980 0.77 -0.9 3,522,327 + -0.7 3,522,647 + BWI76_RS16985 0.26 -1.8
Or see this region's nucleotide sequence