Experiment: RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00960 and BWI76_RS00965 overlap by 4 nucleotides BWI76_RS00965 and BWI76_RS00970 are separated by 16 nucleotides BWI76_RS00970 and BWI76_RS00975 are separated by 60 nucleotides
BWI76_RS00960: BWI76_RS00960 - acetolactate synthase 2 catalytic subunit, at 195,387 to 197,033
_RS00960
BWI76_RS00965: BWI76_RS00965 - acetolactate synthase 2 small subunit, at 197,030 to 197,287
_RS00965
BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233
_RS00970
BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144
_RS00975
Position (kb) 197
198
199 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 196.541 kb on - strand, within BWI76_RS00960 at 196.597 kb on + strand at 196.605 kb on - strand, within BWI76_RS00960 at 196.754 kb on + strand, within BWI76_RS00960 at 196.869 kb on + strand at 196.933 kb on + strand at 196.941 kb on - strand at 196.973 kb on + strand at 196.981 kb on - strand at 197.236 kb on - strand, within BWI76_RS00965 at 197.330 kb on - strand at 197.383 kb on + strand at 197.399 kb on - strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.686 kb on + strand, within BWI76_RS00970 at 198.044 kb on + strand, within BWI76_RS00970 at 198.112 kb on + strand, within BWI76_RS00970 at 198.168 kb on + strand at 198.297 kb on + strand at 198.442 kb on + strand at 198.442 kb on + strand at 198.543 kb on + strand, within BWI76_RS00975 at 198.551 kb on - strand, within BWI76_RS00975 at 198.608 kb on + strand, within BWI76_RS00975 at 198.636 kb on - strand, within BWI76_RS00975 at 198.648 kb on + strand, within BWI76_RS00975 at 198.849 kb on + strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 199.017 kb on - strand, within BWI76_RS00975 at 199.040 kb on + strand, within BWI76_RS00975 at 199.043 kb on - strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5 x; and Iron (II) 0.209 mg/mL remove 196,541 - BWI76_RS00960 0.70 -1.2 196,597 + +0.9 196,605 - BWI76_RS00960 0.74 -2.3 196,754 + BWI76_RS00960 0.83 +0.5 196,869 + -0.3 196,933 + -0.3 196,941 - -1.9 196,973 + -0.2 196,981 - -2.0 197,236 - BWI76_RS00965 0.80 -1.5 197,330 - -1.8 197,383 + -0.1 197,399 - BWI76_RS00970 0.10 -1.9 197,484 + BWI76_RS00970 0.19 -0.7 197,484 + BWI76_RS00970 0.19 -0.2 197,540 + BWI76_RS00970 0.25 -0.2 197,540 + BWI76_RS00970 0.25 +0.3 197,686 + BWI76_RS00970 0.41 -1.9 198,044 + BWI76_RS00970 0.80 +0.3 198,112 + BWI76_RS00970 0.87 -1.4 198,168 + -0.3 198,297 + -2.5 198,442 + -2.7 198,442 + -2.2 198,543 + BWI76_RS00975 0.13 -2.5 198,551 - BWI76_RS00975 0.14 -2.3 198,608 + BWI76_RS00975 0.17 -2.2 198,636 - BWI76_RS00975 0.18 -3.4 198,648 + BWI76_RS00975 0.19 -1.8 198,849 + BWI76_RS00975 0.30 -1.0 198,850 - BWI76_RS00975 0.30 -3.1 198,850 - BWI76_RS00975 0.30 -2.5 199,017 - BWI76_RS00975 0.39 -4.9 199,040 + BWI76_RS00975 0.40 -1.5 199,043 - BWI76_RS00975 0.40 -2.7 199,226 + BWI76_RS00975 0.50 -2.5 199,226 + BWI76_RS00975 0.50 -2.2 199,226 + BWI76_RS00975 0.50 -3.8 199,226 + BWI76_RS00975 0.50 +0.5
Or see this region's nucleotide sequence