Experiment: Survival in PBS at 22C, experiment2, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4492 and SO4494.1 are separated by 446 nucleotides SO4494.1 and SO4494 overlap by 31 nucleotides SO4494 and Sp5SF are separated by 267 nucleotides Sp5SF and tRNA-Thr-3 are separated by 31 nucleotides tRNA-Thr-3 and SO_r028 are separated by 28 nucleotides
SO4492: SO4492 - conserved hypothetical protein (NCBI ptt file), at 4,677,431 to 4,677,943
SO4492
SO4494.1: SO4494.1 - hypothetical protein (NCBI ptt file), at 4,678,390 to 4,678,620
SO4494.1
SO4494: SO4494 - hypothetical protein (NCBI ptt file), at 4,678,590 to 4,678,814
SO4494
SO_r019: Sp5SF - 5S ribosomal RNA (RefSeq), at 4,679,082 to 4,679,201
Sp5SF
SO_t101: tRNA-Thr-3 - tRNA-Thr (RefSeq), at 4,679,233 to 4,679,308
tRNA-Thr-3
SO_r028: SO_r028 - 5S ribosomal RNA (RefSeq), at 4,679,337 to 4,679,456
_r028
Position (kb)
4678
4679 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4677.393 kb on + strand at 4677.393 kb on + strand at 4677.393 kb on + strand at 4677.401 kb on - strand at 4677.459 kb on - strand at 4677.522 kb on + strand, within SO4492 at 4677.532 kb on - strand, within SO4492 at 4677.557 kb on + strand, within SO4492 at 4677.557 kb on + strand, within SO4492 at 4677.723 kb on + strand, within SO4492 at 4677.805 kb on + strand, within SO4492 at 4677.810 kb on + strand, within SO4492 at 4677.932 kb on - strand at 4677.973 kb on - strand at 4677.992 kb on + strand at 4677.992 kb on + strand at 4678.011 kb on - strand at 4678.049 kb on + strand at 4678.286 kb on + strand at 4678.294 kb on - strand at 4678.324 kb on - strand at 4678.388 kb on + strand at 4678.524 kb on + strand, within SO4494.1 at 4678.524 kb on + strand, within SO4494.1 at 4678.532 kb on - strand, within SO4494.1 at 4678.955 kb on + strand at 4678.968 kb on + strand at 4679.220 kb on + strand at 4679.228 kb on - strand at 4679.228 kb on - strand at 4679.228 kb on - strand at 4679.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 13 day remove 4,677,393 + +0.8 4,677,393 + -0.6 4,677,393 + +0.4 4,677,401 - +1.8 4,677,459 - +3.1 4,677,522 + SO4492 0.18 +0.6 4,677,532 - SO4492 0.20 -0.3 4,677,557 + SO4492 0.25 -1.4 4,677,557 + SO4492 0.25 +1.7 4,677,723 + SO4492 0.57 -1.7 4,677,805 + SO4492 0.73 +0.2 4,677,810 + SO4492 0.74 +0.3 4,677,932 - -0.0 4,677,973 - +0.3 4,677,992 + -0.1 4,677,992 + -0.2 4,678,011 - -1.0 4,678,049 + -0.1 4,678,286 + -0.3 4,678,294 - +2.7 4,678,324 - -0.3 4,678,388 + +4.0 4,678,524 + SO4494.1 0.58 -0.6 4,678,524 + SO4494.1 0.58 +3.6 4,678,532 - SO4494.1 0.61 +0.6 4,678,955 + +1.3 4,678,968 + -2.8 4,679,220 + +0.4 4,679,228 - -2.4 4,679,228 - +1.4 4,679,228 - +0.2 4,679,337 - +1.5
Or see this region's nucleotide sequence