Experiment: Survival in PBS at 22C, experiment2, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3279 and SO3280 overlap by 16 nucleotides SO3280 and SO3282 are separated by 272 nucleotides SO3282 and cydB are separated by 445 nucleotides
SO3279: SO3279 - AcrB/AcrD/AcrF family protein (NCBI ptt file), at 3,418,954 to 3,422,034
SO3279
SO3280: SO3280 - conserved hypothetical protein (NCBI ptt file), at 3,422,019 to 3,422,264
SO3280
SO3282: SO3282 - methyl-accepting chemotaxis protein (NCBI ptt file), at 3,422,537 to 3,424,540
SO3282
SO3285: cydB - cytochrome d ubiquinol oxidase, subunit II (NCBI ptt file), at 3,424,986 to 3,426,125
cydB
Position (kb)
3422
3423
3424
3425 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3421.583 kb on + strand, within SO3279 at 3421.621 kb on - strand, within SO3279 at 3421.621 kb on - strand, within SO3279 at 3421.621 kb on - strand, within SO3279 at 3421.625 kb on + strand, within SO3279 at 3421.630 kb on + strand, within SO3279 at 3421.739 kb on + strand at 3421.834 kb on - strand at 3421.853 kb on + strand at 3421.946 kb on - strand at 3422.056 kb on + strand, within SO3280 at 3422.131 kb on - strand, within SO3280 at 3422.216 kb on - strand, within SO3280 at 3422.345 kb on + strand at 3422.374 kb on - strand at 3422.395 kb on - strand at 3422.513 kb on + strand at 3422.522 kb on - strand at 3422.603 kb on - strand at 3422.681 kb on + strand at 3422.751 kb on + strand, within SO3282 at 3422.761 kb on + strand, within SO3282 at 3422.837 kb on - strand, within SO3282 at 3422.880 kb on - strand, within SO3282 at 3422.880 kb on - strand, within SO3282 at 3422.904 kb on - strand, within SO3282 at 3423.024 kb on + strand, within SO3282 at 3423.079 kb on + strand, within SO3282 at 3423.079 kb on + strand, within SO3282 at 3423.087 kb on - strand, within SO3282 at 3423.087 kb on - strand, within SO3282 at 3423.090 kb on - strand, within SO3282 at 3423.103 kb on + strand, within SO3282 at 3423.104 kb on + strand, within SO3282 at 3423.119 kb on + strand, within SO3282 at 3423.247 kb on - strand, within SO3282 at 3423.248 kb on - strand, within SO3282 at 3423.262 kb on + strand, within SO3282 at 3423.302 kb on - strand, within SO3282 at 3423.309 kb on + strand, within SO3282 at 3423.317 kb on - strand, within SO3282 at 3423.317 kb on - strand, within SO3282 at 3423.342 kb on - strand, within SO3282 at 3423.466 kb on + strand, within SO3282 at 3423.508 kb on + strand, within SO3282 at 3423.526 kb on - strand, within SO3282 at 3423.529 kb on - strand, within SO3282 at 3423.554 kb on - strand, within SO3282 at 3423.562 kb on - strand, within SO3282 at 3423.564 kb on + strand, within SO3282 at 3423.564 kb on + strand, within SO3282 at 3423.566 kb on + strand, within SO3282 at 3423.572 kb on - strand, within SO3282 at 3423.574 kb on - strand, within SO3282 at 3423.574 kb on - strand, within SO3282 at 3423.574 kb on - strand, within SO3282 at 3423.712 kb on + strand, within SO3282 at 3423.717 kb on + strand, within SO3282 at 3423.717 kb on + strand, within SO3282 at 3423.717 kb on + strand, within SO3282 at 3423.720 kb on - strand, within SO3282 at 3423.722 kb on + strand, within SO3282 at 3423.725 kb on - strand, within SO3282 at 3423.729 kb on + strand, within SO3282 at 3423.729 kb on + strand, within SO3282 at 3423.729 kb on + strand, within SO3282 at 3423.729 kb on + strand, within SO3282 at 3423.730 kb on - strand, within SO3282 at 3423.737 kb on - strand, within SO3282 at 3423.875 kb on - strand, within SO3282 at 3423.944 kb on + strand, within SO3282 at 3423.944 kb on + strand, within SO3282 at 3423.944 kb on + strand, within SO3282 at 3423.952 kb on - strand, within SO3282 at 3423.952 kb on - strand, within SO3282 at 3423.952 kb on - strand, within SO3282 at 3423.952 kb on - strand, within SO3282 at 3423.952 kb on - strand, within SO3282 at 3423.981 kb on + strand, within SO3282 at 3424.053 kb on + strand, within SO3282 at 3424.150 kb on + strand, within SO3282 at 3424.171 kb on + strand, within SO3282 at 3424.234 kb on - strand, within SO3282 at 3424.445 kb on + strand at 3424.603 kb on + strand at 3424.636 kb on + strand at 3424.978 kb on + strand at 3424.982 kb on + strand at 3424.986 kb on - strand at 3425.000 kb on - strand at 3425.067 kb on + strand at 3425.067 kb on + strand at 3425.072 kb on + strand at 3425.075 kb on - strand at 3425.075 kb on - strand at 3425.167 kb on - strand, within cydB at 3425.211 kb on + strand, within cydB at 3425.299 kb on + strand, within cydB at 3425.398 kb on + strand, within cydB at 3425.398 kb on + strand, within cydB at 3425.401 kb on - strand, within cydB at 3425.406 kb on - strand, within cydB at 3425.406 kb on - strand, within cydB at 3425.406 kb on - strand, within cydB at 3425.406 kb on - strand, within cydB
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 13 day remove 3,421,583 + SO3279 0.85 -0.1 3,421,621 - SO3279 0.87 -0.6 3,421,621 - SO3279 0.87 +0.8 3,421,621 - SO3279 0.87 -0.7 3,421,625 + SO3279 0.87 +0.3 3,421,630 + SO3279 0.87 +1.7 3,421,739 + +0.0 3,421,834 - -0.0 3,421,853 + +0.1 3,421,946 - -0.5 3,422,056 + SO3280 0.15 +0.4 3,422,131 - SO3280 0.46 +0.1 3,422,216 - SO3280 0.80 +2.3 3,422,345 + +0.6 3,422,374 - -1.9 3,422,395 - -2.0 3,422,513 + +0.7 3,422,522 - -0.1 3,422,603 - -2.2 3,422,681 + +0.7 3,422,751 + SO3282 0.11 -2.7 3,422,761 + SO3282 0.11 -0.4 3,422,837 - SO3282 0.15 +1.0 3,422,880 - SO3282 0.17 -1.2 3,422,880 - SO3282 0.17 -0.8 3,422,904 - SO3282 0.18 +0.3 3,423,024 + SO3282 0.24 -1.9 3,423,079 + SO3282 0.27 -0.4 3,423,079 + SO3282 0.27 -0.2 3,423,087 - SO3282 0.27 -1.9 3,423,087 - SO3282 0.27 -1.0 3,423,090 - SO3282 0.28 -0.5 3,423,103 + SO3282 0.28 -0.2 3,423,104 + SO3282 0.28 -1.7 3,423,119 + SO3282 0.29 -0.1 3,423,247 - SO3282 0.35 -0.0 3,423,248 - SO3282 0.35 +0.3 3,423,262 + SO3282 0.36 -0.4 3,423,302 - SO3282 0.38 -0.6 3,423,309 + SO3282 0.39 -0.3 3,423,317 - SO3282 0.39 -0.7 3,423,317 - SO3282 0.39 -0.6 3,423,342 - SO3282 0.40 -1.6 3,423,466 + SO3282 0.46 -0.7 3,423,508 + SO3282 0.48 +0.3 3,423,526 - SO3282 0.49 -0.4 3,423,529 - SO3282 0.50 +0.2 3,423,554 - SO3282 0.51 -1.0 3,423,562 - SO3282 0.51 -0.5 3,423,564 + SO3282 0.51 -1.1 3,423,564 + SO3282 0.51 -1.3 3,423,566 + SO3282 0.51 -0.7 3,423,572 - SO3282 0.52 -0.8 3,423,574 - SO3282 0.52 -0.5 3,423,574 - SO3282 0.52 -2.2 3,423,574 - SO3282 0.52 -0.5 3,423,712 + SO3282 0.59 -3.3 3,423,717 + SO3282 0.59 -2.1 3,423,717 + SO3282 0.59 -0.4 3,423,717 + SO3282 0.59 -0.6 3,423,720 - SO3282 0.59 -2.7 3,423,722 + SO3282 0.59 -0.8 3,423,725 - SO3282 0.59 -0.9 3,423,729 + SO3282 0.59 -0.3 3,423,729 + SO3282 0.59 +0.1 3,423,729 + SO3282 0.59 -1.3 3,423,729 + SO3282 0.59 +0.5 3,423,730 - SO3282 0.60 +0.6 3,423,737 - SO3282 0.60 -0.9 3,423,875 - SO3282 0.67 -0.5 3,423,944 + SO3282 0.70 -1.4 3,423,944 + SO3282 0.70 -0.5 3,423,944 + SO3282 0.70 -0.7 3,423,952 - SO3282 0.71 +0.5 3,423,952 - SO3282 0.71 -1.1 3,423,952 - SO3282 0.71 -0.8 3,423,952 - SO3282 0.71 -0.7 3,423,952 - SO3282 0.71 -2.0 3,423,981 + SO3282 0.72 -0.6 3,424,053 + SO3282 0.76 -0.2 3,424,150 + SO3282 0.80 -0.9 3,424,171 + SO3282 0.82 -1.4 3,424,234 - SO3282 0.85 -1.4 3,424,445 + +1.6 3,424,603 + +0.4 3,424,636 + -1.3 3,424,978 + -0.0 3,424,982 + -2.0 3,424,986 - -0.3 3,425,000 - -0.6 3,425,067 + -0.7 3,425,067 + +0.6 3,425,072 + -1.7 3,425,075 - +0.6 3,425,075 - -1.6 3,425,167 - cydB SO3285 0.16 +0.6 3,425,211 + cydB SO3285 0.20 -2.2 3,425,299 + cydB SO3285 0.27 -1.7 3,425,398 + cydB SO3285 0.36 -1.9 3,425,398 + cydB SO3285 0.36 -2.5 3,425,401 - cydB SO3285 0.36 +0.6 3,425,406 - cydB SO3285 0.37 -0.7 3,425,406 - cydB SO3285 0.37 -2.7 3,425,406 - cydB SO3285 0.37 +1.6 3,425,406 - cydB SO3285 0.37 -0.7
Or see this region's nucleotide sequence