Strain Fitness in Shewanella oneidensis MR-1 around SO3225

Experiment: Survival in PBS at 22C, experiment2, 13 day

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliK and fliJ are separated by 143 nucleotidesfliJ and fliI are separated by 11 nucleotidesfliI and fliH overlap by 32 nucleotidesfliH and fliG overlap by 14 nucleotides SO3223: fliK - flagellar hook-length control protein FliK (NCBI ptt file), at 3,361,106 to 3,362,689 fliK SO3224: fliJ - flagellar protein FliJ (NCBI ptt file), at 3,362,833 to 3,363,276 fliJ SO3225: fliI - flagellum-specific ATP synthase FliI (NCBI ptt file), at 3,363,288 to 3,364,625 fliI SO3226: fliH - flagellar assembly protein FliH (NCBI ptt file), at 3,364,594 to 3,365,544 fliH SO3227: fliG - flagellar motor switch protein FliG (NCBI ptt file), at 3,365,531 to 3,366,577 fliG Position (kb) 3363 3364 3365Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7at 3362.496 kb on - strand, within fliKat 3362.695 kb on + strandat 3362.980 kb on - strand, within fliJat 3363.084 kb on + strand, within fliJat 3363.092 kb on + strand, within fliJat 3363.100 kb on - strand, within fliJat 3363.100 kb on - strand, within fliJat 3363.338 kb on + strandat 3363.502 kb on + strand, within fliIat 3363.502 kb on + strand, within fliIat 3363.510 kb on - strand, within fliIat 3363.510 kb on - strand, within fliIat 3363.510 kb on - strand, within fliIat 3363.510 kb on - strand, within fliIat 3363.608 kb on + strand, within fliIat 3363.613 kb on - strand, within fliIat 3363.690 kb on - strand, within fliIat 3363.707 kb on + strand, within fliIat 3363.707 kb on + strand, within fliIat 3363.935 kb on - strand, within fliIat 3363.935 kb on - strand, within fliIat 3364.071 kb on - strand, within fliIat 3364.096 kb on + strand, within fliIat 3364.235 kb on + strand, within fliIat 3364.314 kb on - strand, within fliIat 3364.553 kb on - strandat 3364.553 kb on - strandat 3365.035 kb on - strand, within fliHat 3365.071 kb on - strand, within fliHat 3365.076 kb on + strand, within fliHat 3365.084 kb on - strand, within fliHat 3365.084 kb on - strand, within fliHat 3365.113 kb on - strand, within fliHat 3365.204 kb on - strand, within fliHat 3365.239 kb on + strand, within fliHat 3365.246 kb on + strand, within fliHat 3365.249 kb on - strand, within fliHat 3365.264 kb on - strand, within fliHat 3365.292 kb on + strand, within fliHat 3365.299 kb on - strand, within fliHat 3365.300 kb on - strand, within fliHat 3365.417 kb on - strand, within fliHat 3365.417 kb on - strand, within fliHat 3365.583 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 13 day
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3,362,496 - fliK SO3223 0.88 +5.3
3,362,695 + +3.0
3,362,980 - fliJ SO3224 0.33 +3.5
3,363,084 + fliJ SO3224 0.57 +3.1
3,363,092 + fliJ SO3224 0.58 +2.4
3,363,100 - fliJ SO3224 0.60 +4.2
3,363,100 - fliJ SO3224 0.60 +2.3
3,363,338 + +1.1
3,363,502 + fliI SO3225 0.16 +3.5
3,363,502 + fliI SO3225 0.16 +2.8
3,363,510 - fliI SO3225 0.17 +3.4
3,363,510 - fliI SO3225 0.17 +1.4
3,363,510 - fliI SO3225 0.17 +2.1
3,363,510 - fliI SO3225 0.17 +2.1
3,363,608 + fliI SO3225 0.24 +4.7
3,363,613 - fliI SO3225 0.24 +3.5
3,363,690 - fliI SO3225 0.30 +3.6
3,363,707 + fliI SO3225 0.31 +1.4
3,363,707 + fliI SO3225 0.31 +1.9
3,363,935 - fliI SO3225 0.48 +2.2
3,363,935 - fliI SO3225 0.48 +1.7
3,364,071 - fliI SO3225 0.59 +1.5
3,364,096 + fliI SO3225 0.60 +3.5
3,364,235 + fliI SO3225 0.71 +1.7
3,364,314 - fliI SO3225 0.77 +1.3
3,364,553 - -0.6
3,364,553 - +2.7
3,365,035 - fliH SO3226 0.46 +0.3
3,365,071 - fliH SO3226 0.50 -1.0
3,365,076 + fliH SO3226 0.51 +1.8
3,365,084 - fliH SO3226 0.52 +2.1
3,365,084 - fliH SO3226 0.52 +2.4
3,365,113 - fliH SO3226 0.55 +1.6
3,365,204 - fliH SO3226 0.64 +2.5
3,365,239 + fliH SO3226 0.68 +0.3
3,365,246 + fliH SO3226 0.69 +3.6
3,365,249 - fliH SO3226 0.69 +4.4
3,365,264 - fliH SO3226 0.70 +3.8
3,365,292 + fliH SO3226 0.73 +6.0
3,365,299 - fliH SO3226 0.74 +1.3
3,365,300 - fliH SO3226 0.74 +4.1
3,365,417 - fliH SO3226 0.87 +1.6
3,365,417 - fliH SO3226 0.87 +0.8
3,365,583 + +6.9

Or see this region's nucleotide sequence