Experiment: Survival in PBS at 22C, experiment2, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheB-3 and SO3207 are separated by 20 nucleotides SO3207 and cheZ are separated by 15 nucleotides cheZ and cheY-3 are separated by 13 nucleotides
SO3206: cheB-3 - protein-glutamate methylesterase CheB (NCBI ptt file), at 3,345,536 to 3,346,660
cheB-3
SO3207: SO3207 - chemotaxis protein (NCBI ptt file), at 3,346,681 to 3,348,957
SO3207
SO3208: cheZ - chemotaxis protein CheZ (NCBI ptt file), at 3,348,973 to 3,349,710
cheZ
SO3209: cheY-3 - chemotaxis protein CheY (NCBI ptt file), at 3,349,724 to 3,350,107
cheY-3
Position (kb) 3346
3347
3348
3349 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 3345.764 kb on - strand, within cheB-3 at 3345.800 kb on - strand, within cheB-3 at 3345.840 kb on - strand at 3345.996 kb on + strand, within cheB-3 at 3346.075 kb on - strand, within cheB-3 at 3346.101 kb on + strand, within cheB-3 at 3346.209 kb on - strand, within cheB-3 at 3346.369 kb on + strand, within cheB-3 at 3346.378 kb on + strand, within cheB-3 at 3346.421 kb on + strand, within cheB-3 at 3346.434 kb on - strand, within cheB-3 at 3346.475 kb on + strand, within cheB-3 at 3346.527 kb on + strand, within cheB-3 at 3346.535 kb on - strand, within cheB-3 at 3346.730 kb on - strand at 3346.858 kb on - strand at 3346.861 kb on + strand at 3346.873 kb on - strand at 3346.944 kb on + strand, within SO3207 at 3346.993 kb on - strand, within SO3207 at 3347.014 kb on - strand, within SO3207 at 3347.144 kb on + strand, within SO3207 at 3347.152 kb on - strand, within SO3207 at 3347.152 kb on - strand, within SO3207 at 3347.172 kb on - strand, within SO3207 at 3347.172 kb on - strand, within SO3207 at 3347.185 kb on - strand, within SO3207 at 3347.447 kb on + strand, within SO3207 at 3347.447 kb on + strand, within SO3207 at 3347.455 kb on - strand, within SO3207 at 3347.455 kb on - strand, within SO3207 at 3347.504 kb on + strand, within SO3207 at 3347.507 kb on - strand, within SO3207 at 3348.181 kb on - strand, within SO3207 at 3348.325 kb on - strand, within SO3207 at 3348.366 kb on - strand, within SO3207 at 3348.466 kb on + strand, within SO3207 at 3348.575 kb on - strand, within SO3207 at 3348.578 kb on - strand, within SO3207 at 3348.707 kb on + strand, within SO3207 at 3348.712 kb on + strand, within SO3207 at 3348.712 kb on + strand, within SO3207 at 3348.720 kb on - strand, within SO3207 at 3348.720 kb on - strand, within SO3207 at 3348.720 kb on - strand, within SO3207 at 3348.762 kb on + strand at 3348.782 kb on - strand at 3348.799 kb on + strand at 3348.799 kb on + strand at 3348.840 kb on + strand at 3348.922 kb on + strand at 3349.009 kb on + strand at 3349.017 kb on - strand at 3349.017 kb on - strand at 3349.017 kb on - strand at 3349.017 kb on - strand at 3349.113 kb on + strand, within cheZ at 3349.302 kb on + strand, within cheZ at 3349.656 kb on - strand at 3349.666 kb on - strand at 3349.689 kb on + strand at 3349.712 kb on + strand at 3349.713 kb on - strand at 3349.720 kb on - strand at 3349.767 kb on - strand, within cheY-3 at 3349.789 kb on + strand, within cheY-3 at 3349.899 kb on + strand, within cheY-3
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 13 day remove 3,345,764 - cheB-3 SO3206 0.20 -1.0 3,345,800 - cheB-3 SO3206 0.23 -1.9 3,345,840 - -1.6 3,345,996 + cheB-3 SO3206 0.41 +0.1 3,346,075 - cheB-3 SO3206 0.48 -1.0 3,346,101 + cheB-3 SO3206 0.50 -1.4 3,346,209 - cheB-3 SO3206 0.60 +0.1 3,346,369 + cheB-3 SO3206 0.74 -2.4 3,346,378 + cheB-3 SO3206 0.75 -2.0 3,346,421 + cheB-3 SO3206 0.79 -0.0 3,346,434 - cheB-3 SO3206 0.80 -0.8 3,346,475 + cheB-3 SO3206 0.83 -0.0 3,346,527 + cheB-3 SO3206 0.88 +1.8 3,346,535 - cheB-3 SO3206 0.89 -1.2 3,346,730 - +0.6 3,346,858 - +7.8 3,346,861 + +6.9 3,346,873 - +8.9 3,346,944 + SO3207 0.12 +4.3 3,346,993 - SO3207 0.14 +7.7 3,347,014 - SO3207 0.15 +7.1 3,347,144 + SO3207 0.20 +6.8 3,347,152 - SO3207 0.21 +7.0 3,347,152 - SO3207 0.21 +8.3 3,347,172 - SO3207 0.22 +6.4 3,347,172 - SO3207 0.22 +6.6 3,347,185 - SO3207 0.22 +8.8 3,347,447 + SO3207 0.34 +8.0 3,347,447 + SO3207 0.34 +3.9 3,347,455 - SO3207 0.34 +6.9 3,347,455 - SO3207 0.34 +5.7 3,347,504 + SO3207 0.36 +2.3 3,347,507 - SO3207 0.36 +6.7 3,348,181 - SO3207 0.66 +7.4 3,348,325 - SO3207 0.72 +7.4 3,348,366 - SO3207 0.74 +7.5 3,348,466 + SO3207 0.78 +5.1 3,348,575 - SO3207 0.83 +7.7 3,348,578 - SO3207 0.83 +7.2 3,348,707 + SO3207 0.89 +5.9 3,348,712 + SO3207 0.89 +8.8 3,348,712 + SO3207 0.89 +5.7 3,348,720 - SO3207 0.90 +7.3 3,348,720 - SO3207 0.90 +8.0 3,348,720 - SO3207 0.90 +8.3 3,348,762 + +7.1 3,348,782 - +7.8 3,348,799 + +6.6 3,348,799 + +5.6 3,348,840 + +8.4 3,348,922 + +6.0 3,349,009 + +8.5 3,349,017 - -1.6 3,349,017 - +0.8 3,349,017 - -2.9 3,349,017 - -1.9 3,349,113 + cheZ SO3208 0.19 +0.0 3,349,302 + cheZ SO3208 0.45 +2.0 3,349,656 - -1.5 3,349,666 - -0.1 3,349,689 + +2.9 3,349,712 + +1.4 3,349,713 - -0.0 3,349,720 - -1.6 3,349,767 - cheY-3 SO3209 0.11 -1.5 3,349,789 + cheY-3 SO3209 0.17 +0.4 3,349,899 + cheY-3 SO3209 0.46 +0.8
Or see this region's nucleotide sequence