Experiment: Survival in PBS at 22C, experiment2, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaA and SO1337 are separated by 2 nucleotides SO1337 and nhaR are separated by 94 nucleotides nhaR and SO1339 are separated by 83 nucleotides SO1339 and SO1340 overlap by 20 nucleotides SO1340 and nadB are separated by 81 nucleotides
SO1336: nhaA - Na+/H+ antiporter (NCBI ptt file), at 1,393,979 to 1,395,148
nhaA
SO1337: SO1337 - hypothetical protein (NCBI ptt file), at 1,395,151 to 1,395,537
SO1337
SO1338: nhaR - transcriptional activator protein NhaR (NCBI ptt file), at 1,395,632 to 1,396,540
nhaR
SO1339: SO1339 - conserved hypothetical protein (NCBI ptt file), at 1,396,624 to 1,396,872
SO1339
SO1340: SO1340 - hypothetical protein (NCBI ptt file), at 1,396,853 to 1,397,311
SO1340
SO1341: nadB - L-aspartate oxidase (NCBI ptt file), at 1,397,393 to 1,399,006
nadB
Position (kb) 1395
1396
1397 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1394.643 kb on - strand, within nhaA at 1394.683 kb on + strand, within nhaA at 1394.747 kb on + strand, within nhaA at 1394.782 kb on - strand, within nhaA at 1395.047 kb on - strand at 1395.062 kb on + strand at 1395.070 kb on - strand at 1395.194 kb on - strand, within SO1337 at 1395.266 kb on + strand, within SO1337 at 1395.274 kb on - strand, within SO1337 at 1395.421 kb on + strand, within SO1337 at 1395.490 kb on + strand, within SO1337 at 1395.738 kb on - strand, within nhaR at 1395.754 kb on + strand, within nhaR at 1395.872 kb on - strand, within nhaR at 1395.872 kb on - strand, within nhaR at 1396.016 kb on - strand, within nhaR at 1396.036 kb on - strand, within nhaR at 1396.185 kb on + strand, within nhaR at 1396.185 kb on + strand, within nhaR at 1396.193 kb on - strand, within nhaR at 1396.193 kb on - strand, within nhaR at 1396.224 kb on - strand, within nhaR at 1396.259 kb on - strand, within nhaR at 1396.409 kb on - strand, within nhaR at 1396.542 kb on - strand at 1396.575 kb on - strand at 1396.735 kb on - strand, within SO1339 at 1396.972 kb on + strand, within SO1340 at 1397.046 kb on + strand, within SO1340 at 1397.049 kb on - strand, within SO1340 at 1397.054 kb on - strand, within SO1340 at 1397.141 kb on + strand, within SO1340 at 1397.194 kb on + strand, within SO1340 at 1397.194 kb on - strand, within SO1340 at 1397.338 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 13 day remove 1,394,643 - nhaA SO1336 0.57 -1.7 1,394,683 + nhaA SO1336 0.60 -0.3 1,394,747 + nhaA SO1336 0.66 -0.8 1,394,782 - nhaA SO1336 0.69 -2.5 1,395,047 - -1.1 1,395,062 + +1.0 1,395,070 - +0.5 1,395,194 - SO1337 0.11 -0.5 1,395,266 + SO1337 0.30 -0.4 1,395,274 - SO1337 0.32 -0.2 1,395,421 + SO1337 0.70 +1.1 1,395,490 + SO1337 0.88 +0.0 1,395,738 - nhaR SO1338 0.12 -0.0 1,395,754 + nhaR SO1338 0.13 +0.8 1,395,872 - nhaR SO1338 0.26 +1.3 1,395,872 - nhaR SO1338 0.26 -1.7 1,396,016 - nhaR SO1338 0.42 -0.6 1,396,036 - nhaR SO1338 0.44 +0.5 1,396,185 + nhaR SO1338 0.61 +0.5 1,396,185 + nhaR SO1338 0.61 +0.5 1,396,193 - nhaR SO1338 0.62 +0.7 1,396,193 - nhaR SO1338 0.62 +2.7 1,396,224 - nhaR SO1338 0.65 +0.7 1,396,259 - nhaR SO1338 0.69 -0.3 1,396,409 - nhaR SO1338 0.85 +0.5 1,396,542 - -0.9 1,396,575 - -0.5 1,396,735 - SO1339 0.45 +1.7 1,396,972 + SO1340 0.26 -1.6 1,397,046 + SO1340 0.42 +0.7 1,397,049 - SO1340 0.43 +0.1 1,397,054 - SO1340 0.44 +0.9 1,397,141 + SO1340 0.63 +0.7 1,397,194 + SO1340 0.74 +0.3 1,397,194 - SO1340 0.74 +2.9 1,397,338 + -0.2
Or see this region's nucleotide sequence