Experiment: Survival in PBS at 22C, experiment2, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemL and pyrB are separated by 26 nucleotides pyrB and SO1302 are separated by 218 nucleotides
SO1300: hemL - glutamate-1-semialdehyde-2,1-aminomutase (NCBI ptt file), at 1,352,002 to 1,353,294
hemL
SO1301: pyrB - aspartate carbamoyltransferase (NCBI ptt file), at 1,353,321 to 1,354,340
pyrB
SO1302: SO1302 - chloride channel (NCBI ptt file), at 1,354,559 to 1,356,283
SO1302
Position (kb) 1353
1354
1355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1353.380 kb on + strand at 1353.407 kb on + strand at 1353.510 kb on - strand, within pyrB at 1353.533 kb on - strand, within pyrB at 1353.543 kb on - strand, within pyrB at 1353.673 kb on + strand, within pyrB at 1353.767 kb on + strand, within pyrB at 1353.775 kb on - strand, within pyrB at 1353.775 kb on - strand, within pyrB at 1353.885 kb on + strand, within pyrB at 1353.998 kb on - strand, within pyrB at 1354.012 kb on + strand, within pyrB at 1354.098 kb on + strand, within pyrB at 1354.113 kb on - strand, within pyrB at 1354.137 kb on + strand, within pyrB at 1354.183 kb on + strand, within pyrB at 1354.227 kb on - strand, within pyrB at 1354.240 kb on + strand at 1354.246 kb on - strand at 1354.248 kb on - strand at 1354.332 kb on - strand at 1354.402 kb on - strand at 1354.447 kb on - strand at 1354.457 kb on - strand at 1354.460 kb on + strand at 1354.461 kb on + strand at 1354.516 kb on - strand at 1354.537 kb on + strand at 1354.540 kb on - strand at 1354.545 kb on - strand at 1354.545 kb on - strand at 1354.550 kb on - strand at 1354.551 kb on - strand at 1354.557 kb on + strand at 1354.626 kb on + strand at 1354.667 kb on + strand at 1354.672 kb on + strand at 1354.672 kb on + strand at 1354.677 kb on + strand at 1354.680 kb on - strand at 1354.680 kb on - strand at 1354.680 kb on - strand at 1354.685 kb on - strand at 1354.717 kb on + strand at 1354.747 kb on + strand, within SO1302 at 1354.753 kb on - strand, within SO1302 at 1354.767 kb on + strand, within SO1302 at 1354.770 kb on - strand, within SO1302 at 1354.810 kb on - strand, within SO1302 at 1354.844 kb on - strand, within SO1302 at 1354.885 kb on - strand, within SO1302 at 1354.943 kb on + strand, within SO1302 at 1355.172 kb on - strand, within SO1302 at 1355.182 kb on - strand, within SO1302 at 1355.182 kb on - strand, within SO1302 at 1355.189 kb on + strand, within SO1302 at 1355.189 kb on + strand, within SO1302 at 1355.203 kb on - strand, within SO1302 at 1355.308 kb on - strand, within SO1302
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 13 day remove 1,353,380 + +1.8 1,353,407 + +1.8 1,353,510 - pyrB SO1301 0.19 -0.6 1,353,533 - pyrB SO1301 0.21 +2.5 1,353,543 - pyrB SO1301 0.22 -0.0 1,353,673 + pyrB SO1301 0.35 +1.8 1,353,767 + pyrB SO1301 0.44 +0.2 1,353,775 - pyrB SO1301 0.45 -0.2 1,353,775 - pyrB SO1301 0.45 +0.6 1,353,885 + pyrB SO1301 0.55 -0.8 1,353,998 - pyrB SO1301 0.66 +1.6 1,354,012 + pyrB SO1301 0.68 -0.7 1,354,098 + pyrB SO1301 0.76 +0.3 1,354,113 - pyrB SO1301 0.78 +1.2 1,354,137 + pyrB SO1301 0.80 -2.1 1,354,183 + pyrB SO1301 0.85 -0.4 1,354,227 - pyrB SO1301 0.89 -0.9 1,354,240 + +2.1 1,354,246 - +0.4 1,354,248 - -0.2 1,354,332 - +1.3 1,354,402 - +0.6 1,354,447 - +0.3 1,354,457 - +0.2 1,354,460 + -1.0 1,354,461 + +0.9 1,354,516 - -0.3 1,354,537 + +1.2 1,354,540 - +0.8 1,354,545 - +1.5 1,354,545 - -0.6 1,354,550 - -2.9 1,354,551 - -1.2 1,354,557 + -0.2 1,354,626 + -0.7 1,354,667 + +0.8 1,354,672 + +0.8 1,354,672 + -0.6 1,354,677 + +0.5 1,354,680 - +0.3 1,354,680 - +1.7 1,354,680 - -0.5 1,354,685 - +0.0 1,354,717 + -0.2 1,354,747 + SO1302 0.11 -0.9 1,354,753 - SO1302 0.11 -0.1 1,354,767 + SO1302 0.12 -1.9 1,354,770 - SO1302 0.12 -0.5 1,354,810 - SO1302 0.15 +0.1 1,354,844 - SO1302 0.17 -1.2 1,354,885 - SO1302 0.19 -2.1 1,354,943 + SO1302 0.22 -0.2 1,355,172 - SO1302 0.36 -0.5 1,355,182 - SO1302 0.36 +0.7 1,355,182 - SO1302 0.36 +1.0 1,355,189 + SO1302 0.37 +1.1 1,355,189 + SO1302 0.37 -3.0 1,355,203 - SO1302 0.37 +1.8 1,355,308 - SO1302 0.43 -0.5
Or see this region's nucleotide sequence