Experiment: Survival in PBS at 22C, experiment2, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0690 and SO0691 are separated by 400 nucleotides SO0691 and galM are separated by 104 nucleotides galM and galK are separated by 140 nucleotides
SO0690: SO0690 - type II DNA modification methyltransferase, putative (NCBI ptt file), at 708,075 to 708,836
SO0690
SO0691: SO0691 - hypothetical protein (NCBI ptt file), at 709,237 to 709,641
SO0691
SO0693: galM - aldose 1-epimerase (NCBI ptt file), at 709,746 to 710,777
galM
SO0694: galK - galactokinase (NCBI ptt file), at 710,918 to 712,063
galK
Position (kb) 709
710
711 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 708.786 kb on + strand at 708.794 kb on - strand at 708.796 kb on + strand at 708.796 kb on + strand at 708.796 kb on + strand at 708.809 kb on - strand at 708.862 kb on - strand at 708.862 kb on - strand at 708.919 kb on + strand at 708.934 kb on - strand at 709.159 kb on + strand at 709.213 kb on - strand at 709.239 kb on + strand at 709.247 kb on - strand at 709.275 kb on + strand at 709.337 kb on - strand, within SO0691 at 709.337 kb on - strand, within SO0691 at 709.463 kb on - strand, within SO0691 at 709.516 kb on + strand, within SO0691 at 709.521 kb on + strand, within SO0691 at 709.527 kb on + strand, within SO0691 at 709.554 kb on + strand, within SO0691 at 709.554 kb on + strand, within SO0691 at 709.562 kb on - strand, within SO0691 at 709.562 kb on - strand, within SO0691 at 709.665 kb on + strand at 709.671 kb on + strand at 709.883 kb on + strand, within galM at 709.898 kb on + strand, within galM at 709.933 kb on + strand, within galM at 709.941 kb on + strand, within galM at 710.248 kb on - strand, within galM at 710.258 kb on + strand, within galM at 710.258 kb on + strand, within galM at 710.266 kb on - strand, within galM at 710.447 kb on - strand, within galM at 710.459 kb on + strand, within galM at 710.500 kb on - strand, within galM at 710.500 kb on - strand, within galM at 710.560 kb on + strand at 710.615 kb on - strand, within galM at 710.634 kb on - strand, within galM at 710.669 kb on + strand, within galM at 710.684 kb on + strand at 710.684 kb on + strand at 710.744 kb on - strand at 710.745 kb on + strand at 710.955 kb on - strand at 710.957 kb on + strand at 711.031 kb on + strand at 711.039 kb on - strand, within galK at 711.067 kb on - strand, within galK at 711.280 kb on - strand, within galK at 711.282 kb on + strand, within galK at 711.306 kb on + strand, within galK at 711.337 kb on + strand, within galK at 711.391 kb on + strand, within galK at 711.395 kb on + strand, within galK at 711.403 kb on - strand, within galK at 711.488 kb on - strand, within galK at 711.491 kb on + strand, within galK at 711.500 kb on - strand, within galK at 711.603 kb on - strand, within galK at 711.702 kb on + strand, within galK at 711.739 kb on + strand, within galK at 711.744 kb on + strand, within galK at 711.752 kb on - strand, within galK
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 13 day remove 708,786 + +0.6 708,794 - +0.1 708,796 + +0.0 708,796 + +1.2 708,796 + -0.6 708,809 - -0.6 708,862 - +2.4 708,862 - -1.9 708,919 + +0.2 708,934 - -0.4 709,159 + -0.2 709,213 - +0.8 709,239 + -0.3 709,247 - -1.2 709,275 + +0.6 709,337 - SO0691 0.25 -1.4 709,337 - SO0691 0.25 -1.5 709,463 - SO0691 0.56 +0.3 709,516 + SO0691 0.69 -2.2 709,521 + SO0691 0.70 -0.5 709,527 + SO0691 0.72 -2.2 709,554 + SO0691 0.78 -1.5 709,554 + SO0691 0.78 -2.4 709,562 - SO0691 0.80 -0.7 709,562 - SO0691 0.80 -2.5 709,665 + -0.5 709,671 + +0.3 709,883 + galM SO0693 0.13 +0.0 709,898 + galM SO0693 0.15 +0.3 709,933 + galM SO0693 0.18 -0.8 709,941 + galM SO0693 0.19 -2.8 710,248 - galM SO0693 0.49 +0.0 710,258 + galM SO0693 0.50 -1.0 710,258 + galM SO0693 0.50 +0.1 710,266 - galM SO0693 0.50 -0.5 710,447 - galM SO0693 0.68 -2.1 710,459 + galM SO0693 0.69 +0.8 710,500 - galM SO0693 0.73 +0.7 710,500 - galM SO0693 0.73 +1.1 710,560 + +2.4 710,615 - galM SO0693 0.84 +0.2 710,634 - galM SO0693 0.86 -1.5 710,669 + galM SO0693 0.89 -0.2 710,684 + +1.6 710,684 + +1.8 710,744 - -2.0 710,745 + -1.9 710,955 - +2.4 710,957 + +1.3 711,031 + +0.6 711,039 - galK SO0694 0.11 +0.8 711,067 - galK SO0694 0.13 -1.3 711,280 - galK SO0694 0.32 +1.3 711,282 + galK SO0694 0.32 +1.1 711,306 + galK SO0694 0.34 +0.9 711,337 + galK SO0694 0.37 +1.4 711,391 + galK SO0694 0.41 +2.9 711,395 + galK SO0694 0.42 +0.6 711,403 - galK SO0694 0.42 +0.5 711,488 - galK SO0694 0.50 +1.3 711,491 + galK SO0694 0.50 +0.0 711,500 - galK SO0694 0.51 -1.5 711,603 - galK SO0694 0.60 +0.1 711,702 + galK SO0694 0.68 +0.8 711,739 + galK SO0694 0.72 -1.8 711,744 + galK SO0694 0.72 +2.4 711,752 - galK SO0694 0.73 +1.5
Or see this region's nucleotide sequence