Experiment: Survival in PBS at 22C, experiment2, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0456 and SO0458 are separated by 522 nucleotides SO0458 and SO0459 are separated by 317 nucleotides SO0459 and SO0461 are separated by 341 nucleotides SO0461 and SO0462 are separated by 12 nucleotides
SO0456: SO0456 - immunogenic-related protein (NCBI ptt file), at 482,440 to 483,396
SO0456
SO0458: SO0458 - hypothetical protein (NCBI ptt file), at 483,919 to 484,041
SO0458
SO0459: SO0459 - conserved hypothetical protein (NCBI ptt file), at 484,359 to 485,840
SO0459
SO0461: SO0461 - hypothetical protein (NCBI ptt file), at 486,182 to 486,544
SO0461
SO0462: SO0462 - hypothetical protein (NCBI ptt file), at 486,557 to 486,982
SO0462
Position (kb)
484
485
486 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 483.360 kb on - strand at 483.360 kb on - strand at 483.552 kb on + strand at 483.585 kb on + strand at 483.591 kb on - strand at 483.618 kb on - strand at 483.728 kb on + strand at 483.822 kb on + strand at 483.822 kb on + strand at 483.830 kb on - strand at 483.830 kb on - strand at 483.866 kb on - strand at 483.875 kb on + strand at 483.877 kb on - strand at 483.893 kb on - strand at 483.900 kb on + strand at 483.907 kb on + strand at 483.916 kb on + strand at 483.971 kb on - strand, within SO0458 at 484.077 kb on + strand at 484.085 kb on - strand at 484.213 kb on + strand at 484.213 kb on + strand at 484.213 kb on + strand at 484.213 kb on + strand at 484.213 kb on + strand at 484.213 kb on + strand at 484.215 kb on + strand at 484.221 kb on - strand at 484.221 kb on - strand at 484.221 kb on - strand at 484.240 kb on - strand at 484.246 kb on + strand at 484.246 kb on + strand at 484.254 kb on - strand at 484.254 kb on - strand at 484.382 kb on + strand at 484.458 kb on + strand at 484.546 kb on + strand, within SO0459 at 484.546 kb on + strand, within SO0459 at 484.554 kb on - strand, within SO0459 at 484.554 kb on - strand, within SO0459 at 484.554 kb on - strand, within SO0459 at 484.590 kb on + strand, within SO0459 at 484.598 kb on - strand, within SO0459 at 484.660 kb on - strand, within SO0459 at 484.667 kb on + strand, within SO0459 at 484.758 kb on + strand, within SO0459 at 484.820 kb on + strand, within SO0459 at 484.864 kb on + strand, within SO0459 at 484.952 kb on + strand, within SO0459 at 485.015 kb on - strand, within SO0459 at 485.026 kb on + strand, within SO0459 at 485.026 kb on + strand, within SO0459 at 485.065 kb on + strand, within SO0459 at 485.147 kb on + strand, within SO0459 at 485.147 kb on + strand, within SO0459 at 485.147 kb on + strand, within SO0459 at 485.155 kb on - strand, within SO0459 at 485.155 kb on - strand, within SO0459 at 485.155 kb on - strand, within SO0459 at 485.160 kb on - strand, within SO0459 at 485.281 kb on - strand, within SO0459 at 485.282 kb on - strand, within SO0459 at 485.357 kb on + strand, within SO0459 at 485.357 kb on + strand, within SO0459 at 485.418 kb on + strand, within SO0459 at 485.471 kb on + strand, within SO0459 at 485.479 kb on - strand, within SO0459 at 485.479 kb on - strand, within SO0459 at 485.479 kb on - strand, within SO0459 at 485.482 kb on + strand, within SO0459 at 485.550 kb on + strand, within SO0459 at 485.615 kb on + strand, within SO0459 at 485.621 kb on - strand, within SO0459 at 485.640 kb on - strand, within SO0459 at 485.714 kb on + strand at 485.714 kb on + strand at 485.846 kb on + strand at 485.854 kb on - strand at 485.865 kb on + strand at 485.865 kb on + strand at 485.865 kb on + strand at 485.865 kb on + strand at 485.865 kb on + strand at 485.877 kb on + strand at 485.920 kb on + strand at 485.933 kb on + strand at 485.981 kb on - strand at 485.981 kb on - strand at 485.981 kb on - strand at 485.981 kb on - strand at 485.981 kb on - strand at 486.258 kb on + strand, within SO0461 at 486.258 kb on + strand, within SO0461 at 486.266 kb on - strand, within SO0461 at 486.266 kb on - strand, within SO0461 at 486.266 kb on - strand, within SO0461 at 486.365 kb on - strand, within SO0461 at 486.371 kb on + strand, within SO0461 at 486.374 kb on - strand, within SO0461 at 486.374 kb on - strand, within SO0461 at 486.376 kb on + strand, within SO0461 at 486.379 kb on - strand, within SO0461 at 486.379 kb on - strand, within SO0461 at 486.384 kb on - strand, within SO0461 at 486.391 kb on + strand, within SO0461 at 486.391 kb on + strand, within SO0461 at 486.414 kb on - strand, within SO0461 at 486.443 kb on - strand, within SO0461 at 486.502 kb on + strand, within SO0461 at 486.512 kb on + strand at 486.520 kb on - strand at 486.520 kb on - strand at 486.569 kb on + strand at 486.569 kb on + strand at 486.570 kb on + strand at 486.580 kb on + strand at 486.685 kb on - strand, within SO0462 at 486.702 kb on + strand, within SO0462 at 486.726 kb on + strand, within SO0462 at 486.782 kb on + strand, within SO0462 at 486.810 kb on + strand, within SO0462
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 13 day remove 483,360 - +0.6 483,360 - +4.1 483,552 + +2.5 483,585 + +1.3 483,591 - +0.6 483,618 - -0.5 483,728 + +0.5 483,822 + -0.2 483,822 + -0.2 483,830 - +0.1 483,830 - -2.9 483,866 - +0.2 483,875 + -1.7 483,877 - +0.5 483,893 - -0.5 483,900 + -0.6 483,907 + +0.4 483,916 + -0.6 483,971 - SO0458 0.42 -0.5 484,077 + +1.2 484,085 - +1.8 484,213 + +1.3 484,213 + +1.7 484,213 + -1.0 484,213 + +1.9 484,213 + +2.1 484,213 + -0.3 484,215 + -1.4 484,221 - -1.8 484,221 - +0.5 484,221 - +0.9 484,240 - -1.5 484,246 + -0.9 484,246 + +0.4 484,254 - -2.1 484,254 - -1.2 484,382 + -0.7 484,458 + +0.2 484,546 + SO0459 0.13 -0.2 484,546 + SO0459 0.13 -1.4 484,554 - SO0459 0.13 +1.1 484,554 - SO0459 0.13 -1.2 484,554 - SO0459 0.13 -0.8 484,590 + SO0459 0.16 +0.0 484,598 - SO0459 0.16 -0.4 484,660 - SO0459 0.20 +1.5 484,667 + SO0459 0.21 +0.2 484,758 + SO0459 0.27 -3.7 484,820 + SO0459 0.31 -0.2 484,864 + SO0459 0.34 -0.8 484,952 + SO0459 0.40 -0.4 485,015 - SO0459 0.44 -0.5 485,026 + SO0459 0.45 -0.9 485,026 + SO0459 0.45 -0.7 485,065 + SO0459 0.48 -1.5 485,147 + SO0459 0.53 +0.2 485,147 + SO0459 0.53 -0.5 485,147 + SO0459 0.53 -1.6 485,155 - SO0459 0.54 -1.8 485,155 - SO0459 0.54 +0.3 485,155 - SO0459 0.54 +0.0 485,160 - SO0459 0.54 +0.4 485,281 - SO0459 0.62 +1.0 485,282 - SO0459 0.62 -1.0 485,357 + SO0459 0.67 -1.4 485,357 + SO0459 0.67 -0.0 485,418 + SO0459 0.71 +1.0 485,471 + SO0459 0.75 +0.2 485,479 - SO0459 0.76 +0.2 485,479 - SO0459 0.76 -1.9 485,479 - SO0459 0.76 -2.0 485,482 + SO0459 0.76 -1.2 485,550 + SO0459 0.80 -1.2 485,615 + SO0459 0.85 +0.5 485,621 - SO0459 0.85 +1.4 485,640 - SO0459 0.86 -0.0 485,714 + +1.1 485,714 + +0.6 485,846 + -0.6 485,854 - -0.8 485,865 + -0.2 485,865 + -0.3 485,865 + -0.9 485,865 + +0.3 485,865 + +0.3 485,877 + -1.2 485,920 + -1.6 485,933 + -2.2 485,981 - +0.0 485,981 - +1.4 485,981 - +0.2 485,981 - +0.2 485,981 - +0.8 486,258 + SO0461 0.21 +1.8 486,258 + SO0461 0.21 +0.3 486,266 - SO0461 0.23 +0.4 486,266 - SO0461 0.23 +0.8 486,266 - SO0461 0.23 -1.0 486,365 - SO0461 0.50 -0.5 486,371 + SO0461 0.52 +1.1 486,374 - SO0461 0.53 -1.2 486,374 - SO0461 0.53 +0.8 486,376 + SO0461 0.53 +0.3 486,379 - SO0461 0.54 -0.2 486,379 - SO0461 0.54 -0.1 486,384 - SO0461 0.56 -0.5 486,391 + SO0461 0.58 +0.8 486,391 + SO0461 0.58 -0.8 486,414 - SO0461 0.64 +0.3 486,443 - SO0461 0.72 +1.5 486,502 + SO0461 0.88 +0.5 486,512 + +1.1 486,520 - -1.5 486,520 - +0.2 486,569 + +1.9 486,569 + +0.2 486,570 + +0.5 486,580 + +1.0 486,685 - SO0462 0.30 -0.2 486,702 + SO0462 0.34 -0.0 486,726 + SO0462 0.40 +0.2 486,782 + SO0462 0.53 -2.3 486,810 + SO0462 0.59 +0.6
Or see this region's nucleotide sequence