Experiment: Survival in PBS at 22C, experiment1, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgL and SO_3240 are separated by 11 nucleotides SO_3240 and SO3239.2 overlap by 13 nucleotides SO3239.2 and SO3239.3 are separated by 56 nucleotides SO3239.3 and flgJ are separated by 40 nucleotides
SO3239: flgL - flagellar hook-associated protein FlgL (NCBI ptt file), at 3,377,772 to 3,378,983
flgL
SO_3240: SO_3240 - no description, at 3,378,995 to 3,379,288
_3240
SO3239.2: SO3239.2 - hypothetical flagellar protein (NCBI ptt file), at 3,379,276 to 3,379,701
SO3239.2
SO3239.3: SO3239.3 - hypothetical flagellar hook-associated protein (NCBI ptt file), at 3,379,758 to 3,380,918
SO3239.3
SO3241: flgJ - flagellar protein FlgJ (NCBI ptt file), at 3,380,959 to 3,382,113
flgJ
Position (kb)
3379
3380
3381 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 3378.822 kb on - strand, within flgL at 3378.846 kb on + strand, within flgL at 3378.858 kb on - strand, within flgL at 3378.889 kb on + strand at 3378.970 kb on + strand at 3378.978 kb on - strand at 3379.178 kb on + strand, within SO_3240 at 3379.222 kb on + strand, within SO_3240 at 3379.230 kb on - strand, within SO_3240 at 3379.269 kb on + strand at 3379.455 kb on + strand, within SO3239.2 at 3379.476 kb on - strand, within SO3239.2 at 3379.565 kb on + strand, within SO3239.2 at 3379.619 kb on + strand, within SO3239.2 at 3379.619 kb on + strand, within SO3239.2 at 3379.639 kb on - strand, within SO3239.2 at 3379.641 kb on - strand, within SO3239.2 at 3379.747 kb on - strand at 3379.756 kb on + strand at 3379.968 kb on + strand, within SO3239.3 at 3379.982 kb on + strand, within SO3239.3 at 3379.990 kb on - strand, within SO3239.3 at 3380.021 kb on - strand, within SO3239.3 at 3380.034 kb on - strand, within SO3239.3 at 3380.063 kb on + strand, within SO3239.3 at 3380.067 kb on + strand, within SO3239.3 at 3380.134 kb on - strand, within SO3239.3 at 3380.134 kb on - strand, within SO3239.3 at 3380.249 kb on - strand, within SO3239.3 at 3380.281 kb on + strand, within SO3239.3 at 3380.282 kb on - strand, within SO3239.3 at 3380.312 kb on + strand, within SO3239.3 at 3380.390 kb on + strand, within SO3239.3 at 3380.421 kb on - strand, within SO3239.3 at 3380.460 kb on + strand, within SO3239.3 at 3380.501 kb on + strand, within SO3239.3 at 3380.519 kb on - strand, within SO3239.3 at 3380.519 kb on - strand, within SO3239.3 at 3380.519 kb on - strand, within SO3239.3 at 3380.519 kb on - strand, within SO3239.3 at 3380.584 kb on + strand, within SO3239.3 at 3380.640 kb on - strand, within SO3239.3 at 3380.645 kb on + strand, within SO3239.3 at 3380.667 kb on + strand, within SO3239.3 at 3380.699 kb on - strand, within SO3239.3 at 3380.774 kb on - strand, within SO3239.3 at 3380.779 kb on - strand, within SO3239.3 at 3380.824 kb on + strand at 3380.824 kb on + strand at 3380.832 kb on - strand at 3380.832 kb on - strand at 3380.858 kb on - strand at 3380.890 kb on - strand at 3380.890 kb on - strand at 3380.970 kb on + strand at 3381.022 kb on - strand at 3381.074 kb on + strand at 3381.076 kb on + strand, within flgJ at 3381.082 kb on - strand, within flgJ at 3381.532 kb on - strand, within flgJ at 3381.537 kb on - strand, within flgJ at 3381.548 kb on + strand, within flgJ at 3381.548 kb on + strand, within flgJ at 3381.556 kb on - strand, within flgJ at 3381.574 kb on + strand, within flgJ at 3381.700 kb on - strand, within flgJ at 3381.702 kb on + strand, within flgJ at 3381.778 kb on - strand, within flgJ at 3381.800 kb on + strand, within flgJ at 3381.805 kb on + strand, within flgJ at 3381.825 kb on + strand, within flgJ at 3381.828 kb on - strand, within flgJ at 3381.895 kb on - strand, within flgJ
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 13 day remove 3,378,822 - flgL SO3239 0.87 +4.8 3,378,846 + flgL SO3239 0.89 +6.1 3,378,858 - flgL SO3239 0.90 +1.4 3,378,889 + +0.2 3,378,970 + +1.2 3,378,978 - +3.2 3,379,178 + SO_3240 0.62 +1.7 3,379,222 + SO_3240 0.77 +0.3 3,379,230 - SO_3240 0.80 +4.0 3,379,269 + +0.9 3,379,455 + SO3239.2 0.42 +0.3 3,379,476 - SO3239.2 0.47 -1.8 3,379,565 + SO3239.2 0.68 +3.9 3,379,619 + SO3239.2 0.81 +2.0 3,379,619 + SO3239.2 0.81 +5.3 3,379,639 - SO3239.2 0.85 +5.1 3,379,641 - SO3239.2 0.86 +1.9 3,379,747 - +3.6 3,379,756 + +0.9 3,379,968 + SO3239.3 0.18 +1.3 3,379,982 + SO3239.3 0.19 +1.7 3,379,990 - SO3239.3 0.20 +2.4 3,380,021 - SO3239.3 0.23 +4.4 3,380,034 - SO3239.3 0.24 +2.6 3,380,063 + SO3239.3 0.26 +3.3 3,380,067 + SO3239.3 0.27 +3.9 3,380,134 - SO3239.3 0.32 +4.1 3,380,134 - SO3239.3 0.32 +4.0 3,380,249 - SO3239.3 0.42 +0.8 3,380,281 + SO3239.3 0.45 +2.0 3,380,282 - SO3239.3 0.45 +1.9 3,380,312 + SO3239.3 0.48 +0.8 3,380,390 + SO3239.3 0.54 +1.2 3,380,421 - SO3239.3 0.57 +3.2 3,380,460 + SO3239.3 0.60 +1.5 3,380,501 + SO3239.3 0.64 +4.1 3,380,519 - SO3239.3 0.66 +0.2 3,380,519 - SO3239.3 0.66 +0.6 3,380,519 - SO3239.3 0.66 +1.7 3,380,519 - SO3239.3 0.66 +1.1 3,380,584 + SO3239.3 0.71 +4.1 3,380,640 - SO3239.3 0.76 +0.8 3,380,645 + SO3239.3 0.76 +2.6 3,380,667 + SO3239.3 0.78 +0.0 3,380,699 - SO3239.3 0.81 -1.8 3,380,774 - SO3239.3 0.88 +2.8 3,380,779 - SO3239.3 0.88 +3.4 3,380,824 + +1.2 3,380,824 + +2.0 3,380,832 - -0.0 3,380,832 - +2.2 3,380,858 - +2.2 3,380,890 - +1.5 3,380,890 - +0.8 3,380,970 + +1.9 3,381,022 - -1.3 3,381,074 + +1.4 3,381,076 + flgJ SO3241 0.10 +2.0 3,381,082 - flgJ SO3241 0.11 +2.3 3,381,532 - flgJ SO3241 0.50 +1.2 3,381,537 - flgJ SO3241 0.50 +1.8 3,381,548 + flgJ SO3241 0.51 +1.6 3,381,548 + flgJ SO3241 0.51 +2.6 3,381,556 - flgJ SO3241 0.52 +0.6 3,381,574 + flgJ SO3241 0.53 +2.2 3,381,700 - flgJ SO3241 0.64 +2.7 3,381,702 + flgJ SO3241 0.64 +1.1 3,381,778 - flgJ SO3241 0.71 +1.5 3,381,800 + flgJ SO3241 0.73 +0.2 3,381,805 + flgJ SO3241 0.73 +3.3 3,381,825 + flgJ SO3241 0.75 +1.9 3,381,828 - flgJ SO3241 0.75 -0.2 3,381,895 - flgJ SO3241 0.81 +2.3
Or see this region's nucleotide sequence