Experiment: Survival in PBS at 22C, experiment1, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4413 and SO4414 are separated by 4 nucleotides SO4414 and dcuB are separated by 280 nucleotides
SO4413: SO4413 - hypothetical kynureninase (NCBI ptt file), at 4,606,608 to 4,607,744
SO4413
SO4414: SO4414 - conserved domain protein (NCBI ptt file), at 4,607,749 to 4,608,927
SO4414
SO4417: dcuB - anaerobic C4-dicarboxylate membrane transporter (NCBI ptt file), at 4,609,208 to 4,610,551
dcuB
Position (kb)
4607
4608
4609 Strain fitness (log2 ratio)
-2
-1
0
1 at 4606.809 kb on - strand, within SO4413 at 4606.827 kb on + strand, within SO4413 at 4606.845 kb on - strand, within SO4413 at 4606.855 kb on - strand, within SO4413 at 4606.855 kb on - strand, within SO4413 at 4606.917 kb on + strand, within SO4413 at 4606.963 kb on + strand, within SO4413 at 4607.108 kb on - strand, within SO4413 at 4607.180 kb on + strand, within SO4413 at 4607.252 kb on + strand, within SO4413 at 4607.293 kb on + strand, within SO4413 at 4607.298 kb on + strand, within SO4413 at 4607.330 kb on + strand, within SO4413 at 4607.330 kb on + strand, within SO4413 at 4607.338 kb on - strand, within SO4413 at 4607.421 kb on + strand, within SO4413 at 4607.603 kb on - strand, within SO4413 at 4607.604 kb on - strand, within SO4413 at 4607.608 kb on + strand, within SO4413 at 4607.904 kb on + strand, within SO4414 at 4607.912 kb on - strand, within SO4414 at 4607.914 kb on + strand, within SO4414 at 4607.927 kb on + strand, within SO4414 at 4607.941 kb on + strand, within SO4414 at 4607.962 kb on + strand, within SO4414 at 4608.011 kb on - strand, within SO4414 at 4608.030 kb on + strand, within SO4414 at 4608.033 kb on - strand, within SO4414 at 4608.033 kb on - strand, within SO4414 at 4608.040 kb on + strand, within SO4414 at 4608.040 kb on + strand, within SO4414 at 4608.172 kb on + strand, within SO4414 at 4608.206 kb on - strand, within SO4414 at 4608.240 kb on + strand, within SO4414 at 4608.240 kb on + strand, within SO4414 at 4608.248 kb on - strand, within SO4414 at 4608.411 kb on - strand, within SO4414 at 4608.452 kb on - strand, within SO4414 at 4608.496 kb on + strand, within SO4414 at 4608.529 kb on + strand, within SO4414 at 4608.568 kb on + strand, within SO4414 at 4608.591 kb on - strand, within SO4414 at 4608.608 kb on + strand, within SO4414 at 4608.618 kb on + strand, within SO4414 at 4608.671 kb on + strand, within SO4414 at 4608.671 kb on + strand, within SO4414 at 4608.671 kb on + strand, within SO4414 at 4608.679 kb on - strand, within SO4414 at 4608.679 kb on - strand, within SO4414 at 4608.679 kb on - strand, within SO4414 at 4608.679 kb on - strand, within SO4414 at 4608.679 kb on - strand, within SO4414 at 4608.694 kb on - strand, within SO4414 at 4608.720 kb on - strand, within SO4414 at 4608.728 kb on + strand, within SO4414 at 4608.773 kb on + strand, within SO4414 at 4608.785 kb on + strand, within SO4414 at 4608.792 kb on + strand, within SO4414 at 4608.792 kb on + strand, within SO4414 at 4608.797 kb on + strand, within SO4414 at 4608.805 kb on - strand, within SO4414 at 4608.821 kb on - strand at 4608.913 kb on - strand at 4608.913 kb on - strand at 4609.061 kb on + strand at 4609.195 kb on - strand at 4609.257 kb on + strand at 4609.257 kb on + strand at 4609.282 kb on - strand at 4609.283 kb on - strand at 4609.349 kb on + strand, within dcuB at 4609.358 kb on + strand, within dcuB at 4609.402 kb on - strand, within dcuB at 4609.413 kb on + strand, within dcuB at 4609.413 kb on + strand, within dcuB at 4609.413 kb on + strand, within dcuB at 4609.413 kb on + strand, within dcuB at 4609.421 kb on - strand, within dcuB at 4609.421 kb on - strand, within dcuB at 4609.421 kb on - strand, within dcuB at 4609.421 kb on - strand, within dcuB at 4609.484 kb on + strand, within dcuB at 4609.492 kb on - strand, within dcuB at 4609.531 kb on + strand, within dcuB at 4609.594 kb on + strand, within dcuB at 4609.694 kb on + strand, within dcuB at 4609.750 kb on + strand, within dcuB at 4609.751 kb on - strand, within dcuB at 4609.758 kb on - strand, within dcuB at 4609.762 kb on + strand, within dcuB at 4609.767 kb on + strand, within dcuB at 4609.783 kb on - strand, within dcuB at 4609.917 kb on - strand, within dcuB
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 13 day remove 4,606,809 - SO4413 0.18 -0.1 4,606,827 + SO4413 0.19 +1.5 4,606,845 - SO4413 0.21 -0.0 4,606,855 - SO4413 0.22 +0.2 4,606,855 - SO4413 0.22 -1.4 4,606,917 + SO4413 0.27 +0.3 4,606,963 + SO4413 0.31 -0.5 4,607,108 - SO4413 0.44 -0.6 4,607,180 + SO4413 0.50 -1.6 4,607,252 + SO4413 0.57 -0.0 4,607,293 + SO4413 0.60 -0.4 4,607,298 + SO4413 0.61 +0.4 4,607,330 + SO4413 0.64 -1.2 4,607,330 + SO4413 0.64 +0.2 4,607,338 - SO4413 0.64 -0.8 4,607,421 + SO4413 0.72 +0.3 4,607,603 - SO4413 0.88 -0.6 4,607,604 - SO4413 0.88 -0.3 4,607,608 + SO4413 0.88 +0.1 4,607,904 + SO4414 0.13 -1.1 4,607,912 - SO4414 0.14 -1.7 4,607,914 + SO4414 0.14 -0.7 4,607,927 + SO4414 0.15 -0.6 4,607,941 + SO4414 0.16 -0.5 4,607,962 + SO4414 0.18 -2.2 4,608,011 - SO4414 0.22 +0.3 4,608,030 + SO4414 0.24 -1.8 4,608,033 - SO4414 0.24 -0.8 4,608,033 - SO4414 0.24 -0.4 4,608,040 + SO4414 0.25 +0.8 4,608,040 + SO4414 0.25 -0.8 4,608,172 + SO4414 0.36 +0.3 4,608,206 - SO4414 0.39 -0.1 4,608,240 + SO4414 0.42 +1.0 4,608,240 + SO4414 0.42 -1.6 4,608,248 - SO4414 0.42 +0.2 4,608,411 - SO4414 0.56 -2.2 4,608,452 - SO4414 0.60 -1.5 4,608,496 + SO4414 0.63 -0.4 4,608,529 + SO4414 0.66 -0.1 4,608,568 + SO4414 0.69 -1.0 4,608,591 - SO4414 0.71 -0.6 4,608,608 + SO4414 0.73 -1.4 4,608,618 + SO4414 0.74 -1.5 4,608,671 + SO4414 0.78 -0.0 4,608,671 + SO4414 0.78 -1.1 4,608,671 + SO4414 0.78 -1.3 4,608,679 - SO4414 0.79 -0.2 4,608,679 - SO4414 0.79 -1.6 4,608,679 - SO4414 0.79 -1.5 4,608,679 - SO4414 0.79 -0.5 4,608,679 - SO4414 0.79 +0.3 4,608,694 - SO4414 0.80 +0.3 4,608,720 - SO4414 0.82 -1.2 4,608,728 + SO4414 0.83 -1.8 4,608,773 + SO4414 0.87 -0.3 4,608,785 + SO4414 0.88 -1.3 4,608,792 + SO4414 0.88 -2.2 4,608,792 + SO4414 0.88 -0.4 4,608,797 + SO4414 0.89 -1.3 4,608,805 - SO4414 0.90 +0.1 4,608,821 - -0.9 4,608,913 - -0.6 4,608,913 - -1.0 4,609,061 + -0.1 4,609,195 - -0.9 4,609,257 + +0.2 4,609,257 + -0.1 4,609,282 - +0.7 4,609,283 - +0.6 4,609,349 + dcuB SO4417 0.10 -1.3 4,609,358 + dcuB SO4417 0.11 +0.5 4,609,402 - dcuB SO4417 0.14 -0.3 4,609,413 + dcuB SO4417 0.15 -1.4 4,609,413 + dcuB SO4417 0.15 -0.6 4,609,413 + dcuB SO4417 0.15 -0.6 4,609,413 + dcuB SO4417 0.15 -0.6 4,609,421 - dcuB SO4417 0.16 -0.6 4,609,421 - dcuB SO4417 0.16 +1.4 4,609,421 - dcuB SO4417 0.16 +0.1 4,609,421 - dcuB SO4417 0.16 -0.1 4,609,484 + dcuB SO4417 0.21 -1.0 4,609,492 - dcuB SO4417 0.21 +1.4 4,609,531 + dcuB SO4417 0.24 -2.0 4,609,594 + dcuB SO4417 0.29 -0.2 4,609,694 + dcuB SO4417 0.36 -0.8 4,609,750 + dcuB SO4417 0.40 -1.0 4,609,751 - dcuB SO4417 0.40 -0.6 4,609,758 - dcuB SO4417 0.41 +1.4 4,609,762 + dcuB SO4417 0.41 -0.3 4,609,767 + dcuB SO4417 0.42 -1.3 4,609,783 - dcuB SO4417 0.43 +0.1 4,609,917 - dcuB SO4417 0.53 -0.2
Or see this region's nucleotide sequence