Experiment: Survival in PBS at 22C, experiment1, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tgt and queA are separated by 161 nucleotides queA and SO3115 are separated by 64 nucleotides SO3115 and SO3116 are separated by 184 nucleotides
SO3113: tgt - queuine tRNA-ribosyltransferase (NCBI ptt file), at 3,241,717 to 3,242,841
tgt
SO3114: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 3,243,003 to 3,244,040
queA
SO3115: SO3115 - hypothetical protein (NCBI ptt file), at 3,244,105 to 3,244,314
SO3115
SO3116: SO3116 - conserved hypothetical protein (NCBI ptt file), at 3,244,499 to 3,245,113
SO3116
Position (kb)
3243
3244
3245 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3242.050 kb on + strand, within tgt at 3242.071 kb on + strand, within tgt at 3242.236 kb on - strand, within tgt at 3242.286 kb on - strand, within tgt at 3242.320 kb on + strand, within tgt at 3242.345 kb on + strand, within tgt at 3242.364 kb on + strand, within tgt at 3242.427 kb on + strand, within tgt at 3242.663 kb on - strand, within tgt at 3242.669 kb on + strand, within tgt at 3242.669 kb on + strand, within tgt at 3242.677 kb on - strand, within tgt at 3242.722 kb on - strand, within tgt at 3242.724 kb on - strand, within tgt at 3242.964 kb on + strand at 3242.964 kb on + strand at 3243.226 kb on + strand, within queA at 3243.226 kb on + strand, within queA at 3243.260 kb on - strand, within queA at 3243.353 kb on + strand, within queA at 3243.545 kb on - strand, within queA at 3243.568 kb on - strand, within queA at 3243.612 kb on - strand, within queA at 3243.627 kb on - strand, within queA at 3243.629 kb on + strand, within queA at 3243.751 kb on - strand, within queA at 3243.766 kb on + strand, within queA at 3243.825 kb on - strand, within queA at 3243.975 kb on - strand at 3244.053 kb on + strand at 3244.058 kb on + strand at 3244.058 kb on + strand at 3244.074 kb on + strand at 3244.097 kb on + strand at 3244.126 kb on - strand, within SO3115 at 3244.126 kb on - strand, within SO3115 at 3244.134 kb on - strand, within SO3115 at 3244.222 kb on + strand, within SO3115 at 3244.233 kb on - strand, within SO3115 at 3244.262 kb on + strand, within SO3115 at 3244.376 kb on - strand at 3244.610 kb on + strand, within SO3116 at 3244.726 kb on - strand, within SO3116 at 3244.751 kb on + strand, within SO3116 at 3244.809 kb on + strand, within SO3116 at 3244.809 kb on + strand, within SO3116 at 3244.812 kb on - strand, within SO3116 at 3244.817 kb on - strand, within SO3116 at 3244.888 kb on - strand, within SO3116 at 3244.926 kb on - strand, within SO3116 at 3244.950 kb on + strand, within SO3116 at 3244.959 kb on - strand, within SO3116 at 3244.981 kb on - strand, within SO3116
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 13 day remove 3,242,050 + tgt SO3113 0.30 -1.3 3,242,071 + tgt SO3113 0.31 -2.3 3,242,236 - tgt SO3113 0.46 -1.3 3,242,286 - tgt SO3113 0.51 -0.7 3,242,320 + tgt SO3113 0.54 -2.1 3,242,345 + tgt SO3113 0.56 +0.3 3,242,364 + tgt SO3113 0.58 -0.3 3,242,427 + tgt SO3113 0.63 -0.5 3,242,663 - tgt SO3113 0.84 -1.2 3,242,669 + tgt SO3113 0.85 -0.3 3,242,669 + tgt SO3113 0.85 +0.4 3,242,677 - tgt SO3113 0.85 +1.3 3,242,722 - tgt SO3113 0.89 +0.3 3,242,724 - tgt SO3113 0.90 +0.3 3,242,964 + -2.3 3,242,964 + -0.7 3,243,226 + queA SO3114 0.21 -2.7 3,243,226 + queA SO3114 0.21 -2.7 3,243,260 - queA SO3114 0.25 +1.3 3,243,353 + queA SO3114 0.34 -0.9 3,243,545 - queA SO3114 0.52 -1.1 3,243,568 - queA SO3114 0.54 -0.6 3,243,612 - queA SO3114 0.59 -0.6 3,243,627 - queA SO3114 0.60 -0.9 3,243,629 + queA SO3114 0.60 -0.6 3,243,751 - queA SO3114 0.72 -0.7 3,243,766 + queA SO3114 0.74 -1.3 3,243,825 - queA SO3114 0.79 -0.8 3,243,975 - -0.9 3,244,053 + -0.5 3,244,058 + -0.8 3,244,058 + -1.4 3,244,074 + -1.5 3,244,097 + -1.7 3,244,126 - SO3115 0.10 -2.5 3,244,126 - SO3115 0.10 +1.0 3,244,134 - SO3115 0.14 -1.4 3,244,222 + SO3115 0.56 -0.2 3,244,233 - SO3115 0.61 +2.8 3,244,262 + SO3115 0.75 +0.8 3,244,376 - +1.2 3,244,610 + SO3116 0.18 -0.8 3,244,726 - SO3116 0.37 -0.1 3,244,751 + SO3116 0.41 +2.7 3,244,809 + SO3116 0.50 +1.6 3,244,809 + SO3116 0.50 -0.1 3,244,812 - SO3116 0.51 -1.6 3,244,817 - SO3116 0.52 +0.1 3,244,888 - SO3116 0.63 -0.1 3,244,926 - SO3116 0.69 +0.2 3,244,950 + SO3116 0.73 +0.7 3,244,959 - SO3116 0.75 +0.2 3,244,981 - SO3116 0.78 +0.0
Or see this region's nucleotide sequence