Strain Fitness in Shewanella oneidensis MR-1 around SO1203

Experiment: Survival in PBS at 22C, experiment1, 13 day

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsecG and tRNA-Leu-7 are separated by 9 nucleotidestRNA-Leu-7 and tRNA-Met-6 are separated by 83 nucleotidestRNA-Met-6 and SO1202 are separated by 168 nucleotidesSO1202 and nusA are separated by 29 nucleotidesnusA and infB are separated by 25 nucleotides SO1201: secG - preprotein translocase, SecG subunit (NCBI ptt file), at 1,245,196 to 1,245,531 secG SO_t021: tRNA-Leu-7 - tRNA-Leu (RefSeq), at 1,245,541 to 1,245,627 tRNA-Leu-7 SO_t022: tRNA-Met-6 - tRNA-Met (RefSeq), at 1,245,711 to 1,245,787 tRNA-Met-6 SO1202: SO1202 - conserved hypothetical protein (NCBI ptt file), at 1,245,956 to 1,246,411 SO1202 SO1203: nusA - N utilization substance protein A (NCBI ptt file), at 1,246,441 to 1,247,940 nusA SO1204: infB - translation initiation factor IF-2 (NCBI ptt file), at 1,247,966 to 1,250,623 infB Position (kb) 1246 1247 1248Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7at 1247.560 kb on + strand, within nusAat 1247.779 kb on + strand, within nusAat 1247.833 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 13 day
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1,247,560 + nusA SO1203 0.75 +6.8
1,247,779 + nusA SO1203 0.89 +2.4
1,247,833 + +1.0

Or see this region's nucleotide sequence