Experiment: Survival in PBS at 22C, experiment1, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argD and astA are separated by 126 nucleotides astA and astD are separated by 9 nucleotides
SO0617: argD - acetylornithine aminotransferase (NCBI ptt file), at 643,453 to 644,670
argD
SO0618: astA - arginine N-succinyltransferase (NCBI ptt file), at 644,797 to 645,816
astA
SO0619: astD - Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71) <small>(from data)</small>, at 645,826 to 647,289
astD
Position (kb) 644
645
646 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 643.875 kb on - strand, within argD at 643.949 kb on + strand, within argD at 644.073 kb on + strand, within argD at 644.073 kb on + strand, within argD at 644.073 kb on + strand, within argD at 644.073 kb on + strand, within argD at 644.073 kb on + strand, within argD at 644.081 kb on - strand, within argD at 644.081 kb on - strand, within argD at 644.081 kb on - strand, within argD at 644.081 kb on - strand, within argD at 644.081 kb on - strand, within argD at 644.081 kb on - strand, within argD at 644.081 kb on - strand, within argD at 644.081 kb on - strand, within argD at 644.155 kb on + strand, within argD at 644.160 kb on + strand, within argD at 644.160 kb on + strand, within argD at 644.160 kb on + strand, within argD at 644.306 kb on - strand, within argD at 644.322 kb on - strand, within argD at 644.341 kb on + strand, within argD at 644.368 kb on - strand, within argD at 644.378 kb on + strand, within argD at 644.378 kb on + strand, within argD at 644.378 kb on + strand, within argD at 644.386 kb on - strand, within argD at 644.472 kb on + strand, within argD at 644.665 kb on + strand at 644.728 kb on - strand at 644.766 kb on - strand at 644.776 kb on - strand at 644.800 kb on - strand at 644.804 kb on - strand at 644.817 kb on - strand at 644.878 kb on - strand at 645.084 kb on + strand, within astA at 645.089 kb on + strand, within astA at 645.090 kb on - strand, within astA at 645.092 kb on - strand, within astA at 645.092 kb on - strand, within astA at 645.132 kb on - strand, within astA at 645.133 kb on - strand, within astA at 645.149 kb on - strand, within astA at 645.155 kb on + strand, within astA at 645.170 kb on - strand, within astA at 645.321 kb on + strand, within astA at 645.423 kb on + strand, within astA at 645.423 kb on + strand, within astA at 645.431 kb on - strand, within astA at 645.441 kb on - strand, within astA at 645.481 kb on + strand, within astA at 645.481 kb on + strand, within astA at 645.483 kb on + strand, within astA at 645.503 kb on + strand, within astA at 645.503 kb on + strand, within astA at 645.511 kb on - strand, within astA at 645.511 kb on - strand, within astA at 645.582 kb on - strand, within astA at 645.653 kb on - strand, within astA at 645.742 kb on + strand at 645.760 kb on - strand at 645.844 kb on + strand at 645.864 kb on + strand at 645.890 kb on - strand at 645.939 kb on + strand at 645.947 kb on - strand at 646.032 kb on - strand, within astD at 646.199 kb on - strand, within astD at 646.224 kb on + strand, within astD at 646.292 kb on + strand, within astD at 646.343 kb on + strand, within astD at 646.366 kb on - strand, within astD at 646.390 kb on - strand, within astD at 646.392 kb on + strand, within astD at 646.409 kb on + strand, within astD at 646.555 kb on - strand, within astD at 646.645 kb on + strand, within astD at 646.670 kb on + strand, within astD at 646.679 kb on - strand, within astD at 646.679 kb on - strand, within astD at 646.735 kb on - strand, within astD at 646.745 kb on - strand, within astD at 646.745 kb on - strand, within astD at 646.804 kb on + strand, within astD
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 13 day remove 643,875 - argD SO0617 0.35 -1.3 643,949 + argD SO0617 0.41 -2.6 644,073 + argD SO0617 0.51 -0.8 644,073 + argD SO0617 0.51 +0.2 644,073 + argD SO0617 0.51 -0.1 644,073 + argD SO0617 0.51 -0.9 644,073 + argD SO0617 0.51 -0.7 644,081 - argD SO0617 0.52 -2.2 644,081 - argD SO0617 0.52 -0.1 644,081 - argD SO0617 0.52 -1.0 644,081 - argD SO0617 0.52 -2.1 644,081 - argD SO0617 0.52 -0.6 644,081 - argD SO0617 0.52 -1.0 644,081 - argD SO0617 0.52 -0.3 644,081 - argD SO0617 0.52 -0.9 644,155 + argD SO0617 0.58 -0.3 644,160 + argD SO0617 0.58 -1.9 644,160 + argD SO0617 0.58 -0.6 644,160 + argD SO0617 0.58 +0.3 644,306 - argD SO0617 0.70 -1.1 644,322 - argD SO0617 0.71 +0.2 644,341 + argD SO0617 0.73 -2.1 644,368 - argD SO0617 0.75 -1.0 644,378 + argD SO0617 0.76 -1.2 644,378 + argD SO0617 0.76 -0.8 644,378 + argD SO0617 0.76 -0.2 644,386 - argD SO0617 0.77 -2.1 644,472 + argD SO0617 0.84 -3.1 644,665 + +0.5 644,728 - -2.0 644,766 - -0.2 644,776 - -0.1 644,800 - -1.0 644,804 - -1.2 644,817 - -1.0 644,878 - -1.1 645,084 + astA SO0618 0.28 -1.9 645,089 + astA SO0618 0.29 -1.2 645,090 - astA SO0618 0.29 -0.5 645,092 - astA SO0618 0.29 -1.2 645,092 - astA SO0618 0.29 -0.9 645,132 - astA SO0618 0.33 -2.4 645,133 - astA SO0618 0.33 -1.3 645,149 - astA SO0618 0.35 -2.1 645,155 + astA SO0618 0.35 -0.9 645,170 - astA SO0618 0.37 -0.7 645,321 + astA SO0618 0.51 -2.3 645,423 + astA SO0618 0.61 -1.9 645,423 + astA SO0618 0.61 -0.2 645,431 - astA SO0618 0.62 -1.5 645,441 - astA SO0618 0.63 -1.0 645,481 + astA SO0618 0.67 -1.3 645,481 + astA SO0618 0.67 -1.2 645,483 + astA SO0618 0.67 -1.1 645,503 + astA SO0618 0.69 -1.8 645,503 + astA SO0618 0.69 -0.9 645,511 - astA SO0618 0.70 -1.7 645,511 - astA SO0618 0.70 -0.2 645,582 - astA SO0618 0.77 -0.3 645,653 - astA SO0618 0.84 -0.3 645,742 + -1.0 645,760 - -0.3 645,844 + -1.0 645,864 + +0.6 645,890 - -0.0 645,939 + -0.1 645,947 - +0.3 646,032 - astD SO0619 0.14 -0.1 646,199 - astD SO0619 0.25 +0.4 646,224 + astD SO0619 0.27 -0.3 646,292 + astD SO0619 0.32 -1.2 646,343 + astD SO0619 0.35 -0.8 646,366 - astD SO0619 0.37 +1.4 646,390 - astD SO0619 0.39 -0.5 646,392 + astD SO0619 0.39 -0.2 646,409 + astD SO0619 0.40 +0.5 646,555 - astD SO0619 0.50 -0.5 646,645 + astD SO0619 0.56 -0.8 646,670 + astD SO0619 0.58 -2.8 646,679 - astD SO0619 0.58 -0.9 646,679 - astD SO0619 0.58 -0.3 646,735 - astD SO0619 0.62 -0.3 646,745 - astD SO0619 0.63 -0.8 646,745 - astD SO0619 0.63 +0.1 646,804 + astD SO0619 0.67 +0.3
Or see this region's nucleotide sequence