Experiment: Survival in PBS at 22C, experiment1, 13 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0596 and SO0597 are separated by 128 nucleotides SO0597 and yjeF overlap by 29 nucleotides yjeF and SO0599 are separated by 80 nucleotides SO0599 and amiB are separated by 8 nucleotides
SO0596: SO0596 - ISSod4, transposase (NCBI ptt file), at 621,892 to 623,094
SO0596
SO0597: SO0597 - hypothetical protein (NCBI ptt file), at 623,223 to 623,426
SO0597
SO0598: yjeF - yjeF protein (NCBI ptt file), at 623,398 to 624,897
yjeF
SO0599: SO0599 - conserved hypothetical protein TIGR00150 (NCBI ptt file), at 624,978 to 625,436
SO0599
SO0600: amiB - N-acetylmuramoyl-L-alanine amidase (NCBI ptt file), at 625,445 to 626,836
amiB
Position (kb) 623
624
625 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 623.147 kb on + strand at 623.316 kb on - strand, within SO0597 at 623.322 kb on - strand, within SO0597 at 623.332 kb on - strand, within SO0597 at 623.350 kb on + strand, within SO0597 at 623.350 kb on + strand, within SO0597 at 623.350 kb on + strand, within SO0597 at 623.358 kb on - strand, within SO0597 at 623.358 kb on - strand, within SO0597 at 623.409 kb on - strand at 623.424 kb on - strand at 623.448 kb on + strand at 623.450 kb on - strand at 623.489 kb on - strand at 623.570 kb on - strand, within yjeF at 623.574 kb on + strand, within yjeF at 623.575 kb on - strand, within yjeF at 623.581 kb on - strand, within yjeF at 623.646 kb on - strand, within yjeF at 623.734 kb on - strand, within yjeF at 623.780 kb on + strand, within yjeF at 623.788 kb on - strand, within yjeF at 623.788 kb on - strand, within yjeF at 623.807 kb on + strand, within yjeF at 623.807 kb on + strand, within yjeF at 623.815 kb on - strand, within yjeF at 623.815 kb on - strand, within yjeF at 623.820 kb on - strand, within yjeF at 623.827 kb on - strand, within yjeF at 624.022 kb on + strand, within yjeF at 624.022 kb on + strand, within yjeF at 624.022 kb on + strand, within yjeF at 624.030 kb on - strand, within yjeF at 624.127 kb on - strand, within yjeF at 624.191 kb on - strand, within yjeF at 624.244 kb on + strand, within yjeF at 624.249 kb on + strand, within yjeF at 624.262 kb on - strand, within yjeF at 624.287 kb on - strand, within yjeF at 624.421 kb on + strand, within yjeF at 624.429 kb on - strand, within yjeF at 624.430 kb on + strand, within yjeF at 624.436 kb on - strand, within yjeF at 624.542 kb on + strand, within yjeF at 624.565 kb on - strand, within yjeF at 624.567 kb on + strand, within yjeF at 624.575 kb on - strand, within yjeF at 624.619 kb on - strand, within yjeF at 624.629 kb on - strand, within yjeF at 624.683 kb on + strand, within yjeF at 624.790 kb on + strand at 624.790 kb on + strand at 624.790 kb on + strand at 624.790 kb on + strand at 624.790 kb on + strand at 624.798 kb on - strand at 624.820 kb on + strand at 624.891 kb on + strand at 624.906 kb on + strand at 625.518 kb on + strand at 625.543 kb on + strand at 625.865 kb on - strand, within amiB at 625.886 kb on - strand, within amiB
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 13 day remove 623,147 + +0.5 623,316 - SO0597 0.46 +1.3 623,322 - SO0597 0.49 +1.7 623,332 - SO0597 0.53 +3.0 623,350 + SO0597 0.62 -0.4 623,350 + SO0597 0.62 -1.5 623,350 + SO0597 0.62 -0.6 623,358 - SO0597 0.66 +1.9 623,358 - SO0597 0.66 +0.7 623,409 - -0.1 623,424 - +1.6 623,448 + +2.8 623,450 - +2.8 623,489 - +2.5 623,570 - yjeF SO0598 0.11 +0.6 623,574 + yjeF SO0598 0.12 +2.5 623,575 - yjeF SO0598 0.12 +1.9 623,581 - yjeF SO0598 0.12 +2.5 623,646 - yjeF SO0598 0.17 +1.8 623,734 - yjeF SO0598 0.22 +2.4 623,780 + yjeF SO0598 0.25 -1.5 623,788 - yjeF SO0598 0.26 +1.2 623,788 - yjeF SO0598 0.26 +2.1 623,807 + yjeF SO0598 0.27 +0.2 623,807 + yjeF SO0598 0.27 +2.2 623,815 - yjeF SO0598 0.28 +2.6 623,815 - yjeF SO0598 0.28 +3.1 623,820 - yjeF SO0598 0.28 +3.0 623,827 - yjeF SO0598 0.29 +2.8 624,022 + yjeF SO0598 0.42 +1.3 624,022 + yjeF SO0598 0.42 +2.6 624,022 + yjeF SO0598 0.42 +1.6 624,030 - yjeF SO0598 0.42 +2.7 624,127 - yjeF SO0598 0.49 +4.1 624,191 - yjeF SO0598 0.53 +2.9 624,244 + yjeF SO0598 0.56 -0.3 624,249 + yjeF SO0598 0.57 +0.9 624,262 - yjeF SO0598 0.58 +3.9 624,287 - yjeF SO0598 0.59 +2.6 624,421 + yjeF SO0598 0.68 +1.9 624,429 - yjeF SO0598 0.69 +2.1 624,430 + yjeF SO0598 0.69 +1.8 624,436 - yjeF SO0598 0.69 +1.9 624,542 + yjeF SO0598 0.76 +0.1 624,565 - yjeF SO0598 0.78 +2.3 624,567 + yjeF SO0598 0.78 -1.3 624,575 - yjeF SO0598 0.78 +2.5 624,619 - yjeF SO0598 0.81 +5.2 624,629 - yjeF SO0598 0.82 +1.8 624,683 + yjeF SO0598 0.86 +2.3 624,790 + +2.1 624,790 + +1.8 624,790 + +2.1 624,790 + +0.1 624,790 + -1.5 624,798 - +1.4 624,820 + +2.3 624,891 + -1.4 624,906 + +1.0 625,518 + +1.9 625,543 + +1.8 625,865 - amiB SO0600 0.30 -1.0 625,886 - amiB SO0600 0.32 -0.7
Or see this region's nucleotide sequence