Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22685 and BWI76_RS22690 are separated by 53 nucleotides BWI76_RS22690 and BWI76_RS22695 overlap by 1 nucleotides BWI76_RS22695 and BWI76_RS22700 are separated by 9 nucleotides BWI76_RS22700 and BWI76_RS22705 are separated by 9 nucleotides
BWI76_RS22685: BWI76_RS22685 - hypothetical protein, at 4,726,569 to 4,726,895
_RS22685
BWI76_RS22690: BWI76_RS22690 - adenylyl-sulfate kinase, at 4,726,949 to 4,727,554
_RS22690
BWI76_RS22695: BWI76_RS22695 - sulfate adenylyltransferase, at 4,727,554 to 4,728,981
_RS22695
BWI76_RS22700: BWI76_RS22700 - sulfate adenylyltransferase subunit 2, at 4,728,991 to 4,729,899
_RS22700
BWI76_RS22705: BWI76_RS22705 - siroheme synthase, at 4,729,909 to 4,731,324
_RS22705
Position (kb) 4727
4728
4729 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4726.585 kb on + strand at 4726.723 kb on + strand, within BWI76_RS22685 at 4726.723 kb on + strand, within BWI76_RS22685 at 4726.730 kb on - strand, within BWI76_RS22685 at 4726.731 kb on - strand, within BWI76_RS22685 at 4726.731 kb on - strand, within BWI76_RS22685 at 4726.731 kb on - strand, within BWI76_RS22685 at 4726.731 kb on - strand, within BWI76_RS22685 at 4726.753 kb on + strand, within BWI76_RS22685 at 4726.776 kb on - strand, within BWI76_RS22685 at 4726.817 kb on + strand, within BWI76_RS22685 at 4726.909 kb on + strand at 4726.964 kb on - strand at 4726.980 kb on - strand at 4726.980 kb on - strand at 4727.091 kb on + strand, within BWI76_RS22690 at 4727.106 kb on + strand, within BWI76_RS22690 at 4727.212 kb on + strand, within BWI76_RS22690 at 4727.279 kb on - strand, within BWI76_RS22690 at 4727.339 kb on + strand, within BWI76_RS22690 at 4727.508 kb on - strand at 4727.638 kb on + strand at 4727.638 kb on + strand at 4727.638 kb on + strand at 4727.638 kb on + strand at 4727.638 kb on + strand at 4727.643 kb on + strand at 4727.646 kb on - strand at 4727.646 kb on - strand at 4727.646 kb on - strand at 4727.697 kb on + strand, within BWI76_RS22695 at 4728.156 kb on + strand at 4728.156 kb on + strand, within BWI76_RS22695 at 4728.164 kb on - strand, within BWI76_RS22695 at 4728.164 kb on - strand, within BWI76_RS22695 at 4728.413 kb on - strand, within BWI76_RS22695 at 4728.620 kb on - strand, within BWI76_RS22695 at 4728.766 kb on - strand, within BWI76_RS22695 at 4728.979 kb on - strand at 4728.984 kb on - strand at 4729.054 kb on + strand at 4729.246 kb on + strand, within BWI76_RS22700 at 4729.401 kb on + strand, within BWI76_RS22700 at 4729.678 kb on + strand, within BWI76_RS22700 at 4729.770 kb on + strand, within BWI76_RS22700 at 4729.811 kb on + strand at 4729.894 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x remove 4,726,585 + +0.5 4,726,723 + BWI76_RS22685 0.47 -0.4 4,726,723 + BWI76_RS22685 0.47 -0.3 4,726,730 - BWI76_RS22685 0.49 +0.1 4,726,731 - BWI76_RS22685 0.50 -0.1 4,726,731 - BWI76_RS22685 0.50 +1.9 4,726,731 - BWI76_RS22685 0.50 +0.2 4,726,731 - BWI76_RS22685 0.50 +0.7 4,726,753 + BWI76_RS22685 0.56 +0.2 4,726,776 - BWI76_RS22685 0.63 -0.9 4,726,817 + BWI76_RS22685 0.76 +0.4 4,726,909 + -0.8 4,726,964 - -0.7 4,726,980 - -2.1 4,726,980 - -0.4 4,727,091 + BWI76_RS22690 0.23 -2.0 4,727,106 + BWI76_RS22690 0.26 -2.3 4,727,212 + BWI76_RS22690 0.43 -2.0 4,727,279 - BWI76_RS22690 0.54 -0.1 4,727,339 + BWI76_RS22690 0.64 -2.8 4,727,508 - -3.5 4,727,638 + -0.6 4,727,638 + -1.0 4,727,638 + -0.2 4,727,638 + -0.5 4,727,638 + -1.2 4,727,643 + -1.5 4,727,646 - -0.7 4,727,646 - +0.0 4,727,646 - -0.5 4,727,697 + BWI76_RS22695 0.10 -0.6 4,728,156 + +0.4 4,728,156 + BWI76_RS22695 0.42 -0.4 4,728,164 - BWI76_RS22695 0.43 -0.9 4,728,164 - BWI76_RS22695 0.43 -0.5 4,728,413 - BWI76_RS22695 0.60 -0.1 4,728,620 - BWI76_RS22695 0.75 -1.8 4,728,766 - BWI76_RS22695 0.85 -0.8 4,728,979 - -0.9 4,728,984 - -0.6 4,729,054 + -0.5 4,729,246 + BWI76_RS22700 0.28 -0.1 4,729,401 + BWI76_RS22700 0.45 -0.8 4,729,678 + BWI76_RS22700 0.76 -0.9 4,729,770 + BWI76_RS22700 0.86 -0.7 4,729,811 + -0.7 4,729,894 + -0.5
Or see this region's nucleotide sequence