Strain Fitness in Shewanella oneidensis MR-1 around SO1643

Experiment: Survival in PBS at 4C, experiment2, 13 day

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlpxB and rnhB overlap by 1 nucleotidesrnhB and dnaE are separated by 116 nucleotides SO1642: lpxB - lipid A disaccharide synthase (NCBI ptt file), at 1,724,404 to 1,725,561 lpxB SO1643: rnhB - ribonuclease HII (NCBI ptt file), at 1,725,561 to 1,726,190 rnhB SO1644: dnaE - DNA polymerase III, alpha subunit (NCBI ptt file), at 1,726,307 to 1,729,783 dnaE Position (kb) 1725 1726 1727Strain fitness (log2 ratio) -1 0 1at 1725.594 kb on + strandat 1725.673 kb on - strand, within rnhBat 1725.800 kb on + strand, within rnhBat 1725.800 kb on + strand, within rnhBat 1725.803 kb on - strand, within rnhBat 1725.808 kb on - strand, within rnhBat 1725.867 kb on + strand, within rnhBat 1726.082 kb on + strand, within rnhBat 1726.082 kb on + strand, within rnhBat 1726.090 kb on - strand, within rnhBat 1726.090 kb on - strand, within rnhBat 1726.090 kb on - strand, within rnhB

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Per-strain Table

Position Strand Gene LocusTag Fraction Survival in PBS at 4C, experiment2, 13 day
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1,725,594 + +0.2
1,725,673 - rnhB SO1643 0.18 +0.5
1,725,800 + rnhB SO1643 0.38 -1.2
1,725,800 + rnhB SO1643 0.38 +0.3
1,725,803 - rnhB SO1643 0.38 -0.4
1,725,808 - rnhB SO1643 0.39 +1.2
1,725,867 + rnhB SO1643 0.49 -0.6
1,726,082 + rnhB SO1643 0.83 -0.2
1,726,082 + rnhB SO1643 0.83 -0.6
1,726,090 - rnhB SO1643 0.84 -0.7
1,726,090 - rnhB SO1643 0.84 -1.2
1,726,090 - rnhB SO1643 0.84 -0.6

Or see this region's nucleotide sequence