Experiment: Varel_Bryant_medium_Glucose with Bile salts 0.125 mg/mL; pH 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0203 and BT0204 are separated by 90 nucleotides BT0204 and BT0205 are separated by 114 nucleotides BT0205 and BT0206 are separated by 657 nucleotides
BT0203: BT0203 - histidine biosynthesis bifunctional protein hisB (NCBI ptt file), at 218,282 to 219,406
BT0203
BT0204: BT0204 - conserved hypothetical protein (NCBI ptt file), at 219,497 to 220,006
BT0204
BT0205: BT0205 - glutamine-dependent NAD+ synthetase (NCBI ptt file), at 220,121 to 222,046
BT0205
BT0206: BT0206 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 222,704 to 225,646
BT0206
Position (kb) 220
221
222
223 Strain fitness (log2 ratio) -2
-1
0
1 at 219.172 kb on - strand, within BT0203 at 219.310 kb on - strand at 219.365 kb on - strand at 219.419 kb on + strand at 219.420 kb on - strand at 219.557 kb on - strand, within BT0204 at 219.580 kb on - strand, within BT0204 at 219.592 kb on - strand, within BT0204 at 219.641 kb on + strand, within BT0204 at 219.642 kb on - strand, within BT0204 at 219.712 kb on + strand, within BT0204 at 219.846 kb on + strand, within BT0204 at 219.853 kb on + strand, within BT0204 at 219.854 kb on - strand, within BT0204 at 219.857 kb on + strand, within BT0204 at 219.863 kb on - strand, within BT0204 at 219.863 kb on - strand, within BT0204 at 219.935 kb on - strand, within BT0204 at 219.946 kb on + strand, within BT0204 at 219.947 kb on - strand, within BT0204 at 219.997 kb on - strand at 220.020 kb on - strand at 220.038 kb on - strand at 220.080 kb on - strand at 222.060 kb on - strand at 222.066 kb on - strand at 222.139 kb on - strand at 222.228 kb on - strand at 222.400 kb on - strand at 222.400 kb on - strand at 222.564 kb on - strand at 222.602 kb on - strand at 222.602 kb on - strand at 222.602 kb on - strand at 222.602 kb on - strand at 222.783 kb on - strand at 222.867 kb on + strand at 222.886 kb on + strand at 223.021 kb on - strand, within BT0206 at 223.039 kb on - strand, within BT0206 at 223.039 kb on - strand, within BT0206 at 223.039 kb on - strand, within BT0206 at 223.039 kb on - strand, within BT0206
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Bile salts 0.125 mg/mL; pH 6 remove 219,172 - BT0203 0.79 -2.4 219,310 - -2.4 219,365 - -2.7 219,419 + -0.1 219,420 - -0.3 219,557 - BT0204 0.12 -0.1 219,580 - BT0204 0.16 -2.0 219,592 - BT0204 0.19 -0.3 219,641 + BT0204 0.28 -0.1 219,642 - BT0204 0.28 -0.3 219,712 + BT0204 0.42 -0.0 219,846 + BT0204 0.68 +0.0 219,853 + BT0204 0.70 -0.4 219,854 - BT0204 0.70 +1.1 219,857 + BT0204 0.71 -0.3 219,863 - BT0204 0.72 -0.8 219,863 - BT0204 0.72 +1.3 219,935 - BT0204 0.86 +0.0 219,946 + BT0204 0.88 -1.0 219,947 - BT0204 0.88 +0.2 219,997 - -0.1 220,020 - -1.4 220,038 - +1.2 220,080 - +0.5 222,060 - -0.7 222,066 - -0.5 222,139 - -1.2 222,228 - -2.4 222,400 - -0.9 222,400 - -0.3 222,564 - -0.7 222,602 - -1.1 222,602 - -0.7 222,602 - +1.3 222,602 - -0.0 222,783 - +0.5 222,867 + +0.9 222,886 + +0.3 223,021 - BT0206 0.11 -0.4 223,039 - BT0206 0.11 -0.7 223,039 - BT0206 0.11 -0.8 223,039 - BT0206 0.11 -0.4 223,039 - BT0206 0.11 -2.1
Or see this region's nucleotide sequence