Experiment: Survival in PBS at 22C, experiment2, 6 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2078 and SO2079 are separated by 282 nucleotides SO2079 and SO2080 are separated by 17 nucleotides SO2080 and SO2081 are separated by 263 nucleotides SO2081 and SO2082 are separated by 63 nucleotides SO2082 and SO2083 are separated by 43 nucleotides
SO2078: SO2078 - ISSod4, transposase (NCBI ptt file), at 2,174,195 to 2,175,397
SO2078
SO2079: SO2079 - hypothetical protein (NCBI ptt file), at 2,175,680 to 2,175,982
SO2079
SO2080: SO2080 - hypothetical protein (NCBI ptt file), at 2,176,000 to 2,176,098
SO2080
SO2081: SO2081 - ATP-dependent helicase, DinG family (NCBI ptt file), at 2,176,362 to 2,178,263
SO2081
SO2082: SO2082 - rod shape-determining-related protein (NCBI ptt file), at 2,178,327 to 2,178,827
SO2082
SO2083: SO2083 - methyl-accepting chemotaxis protein (NCBI ptt file), at 2,178,871 to 2,180,388
SO2083
Position (kb)
2176
2177
2178
2179 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2175.452 kb on + strand at 2175.453 kb on + strand at 2175.461 kb on + strand at 2175.461 kb on - strand at 2175.461 kb on - strand at 2175.509 kb on - strand at 2175.562 kb on + strand at 2175.570 kb on - strand at 2175.570 kb on - strand at 2175.581 kb on + strand at 2175.581 kb on + strand at 2175.581 kb on + strand at 2175.589 kb on - strand at 2175.589 kb on - strand at 2175.589 kb on - strand at 2175.589 kb on - strand at 2175.589 kb on - strand at 2175.589 kb on - strand at 2175.621 kb on + strand at 2175.621 kb on + strand at 2175.629 kb on - strand at 2175.629 kb on - strand at 2175.641 kb on + strand at 2175.651 kb on - strand at 2175.724 kb on + strand, within SO2079 at 2175.724 kb on + strand, within SO2079 at 2175.732 kb on - strand, within SO2079 at 2175.732 kb on - strand, within SO2079 at 2175.732 kb on - strand, within SO2079 at 2175.732 kb on - strand, within SO2079 at 2175.764 kb on - strand, within SO2079 at 2175.846 kb on - strand, within SO2079 at 2175.869 kb on + strand, within SO2079 at 2176.006 kb on + strand at 2176.006 kb on + strand at 2176.030 kb on - strand, within SO2080 at 2176.043 kb on + strand, within SO2080 at 2176.045 kb on + strand, within SO2080 at 2176.051 kb on - strand, within SO2080 at 2176.058 kb on + strand, within SO2080 at 2176.066 kb on - strand, within SO2080 at 2176.066 kb on - strand, within SO2080 at 2176.114 kb on + strand at 2176.122 kb on - strand at 2176.165 kb on + strand at 2176.165 kb on + strand at 2176.173 kb on - strand at 2176.183 kb on - strand at 2176.213 kb on + strand at 2176.221 kb on - strand at 2176.257 kb on + strand at 2176.265 kb on - strand at 2176.305 kb on - strand at 2176.404 kb on + strand at 2176.412 kb on - strand at 2176.441 kb on - strand at 2176.515 kb on + strand at 2176.549 kb on - strand at 2176.549 kb on - strand at 2176.849 kb on + strand, within SO2081 at 2176.849 kb on + strand, within SO2081 at 2176.849 kb on + strand, within SO2081 at 2176.849 kb on + strand, within SO2081 at 2176.864 kb on + strand, within SO2081 at 2176.877 kb on - strand, within SO2081 at 2177.029 kb on + strand, within SO2081 at 2177.108 kb on - strand, within SO2081 at 2177.250 kb on - strand, within SO2081 at 2177.386 kb on + strand, within SO2081 at 2177.556 kb on + strand, within SO2081 at 2177.574 kb on - strand, within SO2081 at 2177.725 kb on + strand, within SO2081 at 2177.743 kb on - strand, within SO2081 at 2177.745 kb on + strand, within SO2081 at 2177.748 kb on - strand, within SO2081 at 2177.753 kb on - strand, within SO2081 at 2177.753 kb on - strand, within SO2081 at 2177.846 kb on - strand, within SO2081 at 2177.853 kb on + strand, within SO2081 at 2177.896 kb on - strand, within SO2081 at 2177.929 kb on + strand, within SO2081 at 2177.937 kb on - strand, within SO2081 at 2177.978 kb on + strand, within SO2081 at 2178.002 kb on + strand, within SO2081 at 2178.010 kb on - strand, within SO2081 at 2178.327 kb on - strand at 2178.382 kb on + strand, within SO2082 at 2178.424 kb on - strand, within SO2082 at 2178.487 kb on + strand, within SO2082 at 2178.487 kb on + strand, within SO2082 at 2178.502 kb on - strand, within SO2082 at 2178.510 kb on - strand, within SO2082 at 2178.573 kb on - strand, within SO2082 at 2178.583 kb on - strand, within SO2082 at 2178.595 kb on - strand, within SO2082 at 2178.616 kb on + strand, within SO2082 at 2178.640 kb on + strand, within SO2082 at 2178.665 kb on + strand, within SO2082 at 2178.673 kb on - strand, within SO2082 at 2178.791 kb on + strand at 2178.934 kb on + strand at 2178.942 kb on - strand at 2179.084 kb on - strand, within SO2083 at 2179.189 kb on + strand, within SO2083 at 2179.189 kb on + strand, within SO2083
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment2, 6 day remove 2,175,452 + -0.0 2,175,453 + +1.1 2,175,461 + -0.5 2,175,461 - +0.4 2,175,461 - +0.7 2,175,509 - +0.3 2,175,562 + -0.6 2,175,570 - +1.0 2,175,570 - +0.4 2,175,581 + +1.0 2,175,581 + +0.2 2,175,581 + -0.0 2,175,589 - +0.2 2,175,589 - -0.3 2,175,589 - +0.1 2,175,589 - -0.6 2,175,589 - +0.4 2,175,589 - +0.5 2,175,621 + +0.9 2,175,621 + -1.6 2,175,629 - -0.0 2,175,629 - +0.3 2,175,641 + +0.2 2,175,651 - -0.0 2,175,724 + SO2079 0.15 +1.6 2,175,724 + SO2079 0.15 -0.0 2,175,732 - SO2079 0.17 +0.4 2,175,732 - SO2079 0.17 +0.4 2,175,732 - SO2079 0.17 +0.4 2,175,732 - SO2079 0.17 +0.8 2,175,764 - SO2079 0.28 +0.2 2,175,846 - SO2079 0.55 -0.2 2,175,869 + SO2079 0.62 -0.3 2,176,006 + +1.0 2,176,006 + -0.3 2,176,030 - SO2080 0.30 -0.1 2,176,043 + SO2080 0.43 -0.8 2,176,045 + SO2080 0.45 -1.3 2,176,051 - SO2080 0.52 -1.0 2,176,058 + SO2080 0.59 -0.2 2,176,066 - SO2080 0.67 -0.4 2,176,066 - SO2080 0.67 +1.0 2,176,114 + +0.6 2,176,122 - +0.1 2,176,165 + -2.2 2,176,165 + +0.3 2,176,173 - +0.7 2,176,183 - -0.8 2,176,213 + +0.4 2,176,221 - -1.3 2,176,257 + +0.6 2,176,265 - -0.4 2,176,305 - -0.3 2,176,404 + -0.3 2,176,412 - -0.0 2,176,441 - +1.0 2,176,515 + -2.6 2,176,549 - -0.4 2,176,549 - -0.0 2,176,849 + SO2081 0.26 -1.4 2,176,849 + SO2081 0.26 +0.6 2,176,849 + SO2081 0.26 +0.1 2,176,849 + SO2081 0.26 -0.6 2,176,864 + SO2081 0.26 +0.0 2,176,877 - SO2081 0.27 +0.2 2,177,029 + SO2081 0.35 -0.1 2,177,108 - SO2081 0.39 +0.6 2,177,250 - SO2081 0.47 -0.2 2,177,386 + SO2081 0.54 +1.7 2,177,556 + SO2081 0.63 +0.2 2,177,574 - SO2081 0.64 -1.4 2,177,725 + SO2081 0.72 -0.4 2,177,743 - SO2081 0.73 -0.0 2,177,745 + SO2081 0.73 -2.6 2,177,748 - SO2081 0.73 -2.0 2,177,753 - SO2081 0.73 -1.1 2,177,753 - SO2081 0.73 -0.4 2,177,846 - SO2081 0.78 -0.7 2,177,853 + SO2081 0.78 -1.3 2,177,896 - SO2081 0.81 -0.8 2,177,929 + SO2081 0.82 +0.2 2,177,937 - SO2081 0.83 +0.1 2,177,978 + SO2081 0.85 -0.5 2,178,002 + SO2081 0.86 +0.6 2,178,010 - SO2081 0.87 +0.4 2,178,327 - +0.2 2,178,382 + SO2082 0.11 +1.8 2,178,424 - SO2082 0.19 -1.0 2,178,487 + SO2082 0.32 -0.4 2,178,487 + SO2082 0.32 -0.3 2,178,502 - SO2082 0.35 +2.6 2,178,510 - SO2082 0.37 -0.4 2,178,573 - SO2082 0.49 +0.7 2,178,583 - SO2082 0.51 +0.2 2,178,595 - SO2082 0.53 +0.1 2,178,616 + SO2082 0.58 +0.1 2,178,640 + SO2082 0.62 -2.6 2,178,665 + SO2082 0.67 +0.3 2,178,673 - SO2082 0.69 +1.2 2,178,791 + -0.0 2,178,934 + -0.4 2,178,942 - -0.0 2,179,084 - SO2083 0.14 +0.2 2,179,189 + SO2083 0.21 -0.7 2,179,189 + SO2083 0.21 +0.8
Or see this region's nucleotide sequence