Experiment: Survival in PBS at 22C, experiment1, 6 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1522 and SO1523 are separated by 733 nucleotides SO1523 and grpE are separated by 99 nucleotides grpE and dxs are separated by 117 nucleotides
SO1522: SO1522 - D,L-lactate/pyruvate symporter LctP2 (from data) , at 1,597,090 to 1,598,733
SO1522
SO1523: SO1523 - conserved hypothetical protein (NCBI ptt file), at 1,599,467 to 1,600,345
SO1523
SO1524: grpE - heat shock protein GrpE (NCBI ptt file), at 1,600,445 to 1,601,065
grpE
SO1525: dxs - deoxyxylulose-5-phosphate synthase (NCBI ptt file), at 1,601,183 to 1,603,051
dxs
Position (kb)
1599
1600
1601 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1598.572 kb on + strand at 1598.572 kb on + strand at 1598.599 kb on + strand at 1598.599 kb on + strand at 1598.641 kb on - strand at 1598.690 kb on - strand at 1598.694 kb on + strand at 1598.708 kb on - strand at 1598.735 kb on - strand at 1598.739 kb on + strand at 1598.747 kb on - strand at 1598.747 kb on - strand at 1598.752 kb on - strand at 1598.761 kb on - strand at 1598.854 kb on + strand at 1598.854 kb on + strand at 1598.862 kb on - strand at 1598.870 kb on - strand at 1598.878 kb on + strand at 1598.878 kb on + strand at 1598.886 kb on - strand at 1598.938 kb on - strand at 1598.940 kb on - strand at 1598.953 kb on - strand at 1599.016 kb on - strand at 1599.265 kb on - strand at 1599.265 kb on - strand at 1599.272 kb on - strand at 1599.280 kb on + strand at 1601.014 kb on + strand at 1601.066 kb on + strand at 1601.199 kb on + strand at 1601.207 kb on - strand at 1601.207 kb on - strand at 1601.207 kb on - strand at 1601.207 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 6 day remove 1,598,572 + -0.3 1,598,572 + -0.5 1,598,599 + +0.9 1,598,599 + -0.6 1,598,641 - +0.1 1,598,690 - +0.2 1,598,694 + -0.9 1,598,708 - +0.5 1,598,735 - +0.0 1,598,739 + -0.6 1,598,747 - +0.3 1,598,747 - +2.0 1,598,752 - -0.4 1,598,761 - +0.2 1,598,854 + +0.7 1,598,854 + +0.3 1,598,862 - -1.3 1,598,870 - +0.1 1,598,878 + +1.9 1,598,878 + -1.0 1,598,886 - -0.5 1,598,938 - -3.1 1,598,940 - -1.0 1,598,953 - -0.6 1,599,016 - -1.6 1,599,265 - +0.1 1,599,265 - -0.2 1,599,272 - -0.8 1,599,280 + -0.4 1,601,014 + -1.5 1,601,066 + +0.0 1,601,199 + +1.0 1,601,207 - +0.0 1,601,207 - -2.3 1,601,207 - +0.2 1,601,207 - +1.0
Or see this region's nucleotide sequence