Experiment: Survival in PBS at 22C, experiment1, 6 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3945 and SO_3946 are separated by 35 nucleotides SO_3946 and murA are separated by 364 nucleotides murA and SO3949 are separated by 12 nucleotides SO3949 and SO3950 are separated by 16 nucleotides SO3950 and SO3951 are separated by 5 nucleotides
SO3945: SO3945 - transposase, IS110 family (NCBI ptt file), at 4,091,682 to 4,092,716
SO3945
SO_3946: SO_3946 - no description, at 4,092,752 to 4,093,075
_3946
SO3948: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (NCBI ptt file), at 4,093,440 to 4,094,699
murA
SO3949: SO3949 - BolA/YrbA family protein (NCBI ptt file), at 4,094,712 to 4,094,963
SO3949
SO3950: SO3950 - SpoIIAA family protein (NCBI ptt file), at 4,094,980 to 4,095,264
SO3950
SO3951: SO3951 - conserved hypothetical protein (NCBI ptt file), at 4,095,270 to 4,095,923
SO3951
Position (kb) 4093
4094
4095 Strain fitness (log2 ratio) -2
-1
0
1 at 4093.202 kb on - strand at 4094.810 kb on - strand, within SO3949 at 4094.909 kb on - strand, within SO3949 at 4095.244 kb on - strand at 4095.244 kb on - strand at 4095.274 kb on - strand at 4095.388 kb on - strand, within SO3951 at 4095.664 kb on - strand, within SO3951 at 4095.677 kb on - strand, within SO3951
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 6 day remove 4,093,202 - +0.6 4,094,810 - SO3949 0.39 +0.6 4,094,909 - SO3949 0.78 +0.7 4,095,244 - +1.4 4,095,244 - -0.5 4,095,274 - -1.5 4,095,388 - SO3951 0.18 -1.9 4,095,664 - SO3951 0.60 -2.5 4,095,677 - SO3951 0.62 -2.5
Or see this region's nucleotide sequence