Experiment: Survival in PBS at 22C, experiment1, 6 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nqrD-1 and nqrE-1 are separated by 9 nucleotides nqrE-1 and nqrF-1 are separated by 51 nucleotides nqrF-1 and SO0908 are separated by 97 nucleotides SO0908 and SO0909 are separated by 42 nucleotides
SO0905: nqrD-1 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrD (NCBI ptt file), at 932,313 to 932,933
nqrD-1
SO0906: nqrE-1 - NADH:ubiquinone oxidoreductase, Na translocating, hydrophobic membrane protein NqrE (NCBI ptt file), at 932,943 to 933,551
nqrE-1
SO0907: nqrF-1 - NADH:ubiquinone oxidoreductase, Na translocating, beta subunit (NCBI ptt file), at 933,603 to 934,820
nqrF-1
SO0908: SO0908 - conserved domain protein (NCBI ptt file), at 934,918 to 935,235
SO0908
SO0909: SO0909 - hypothetical protein (NCBI ptt file), at 935,278 to 935,841
SO0909
Position (kb)
933
934
935 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 932.652 kb on + strand, within nqrD-1 at 932.660 kb on - strand, within nqrD-1 at 932.664 kb on + strand, within nqrD-1 at 932.684 kb on + strand, within nqrD-1 at 932.700 kb on + strand, within nqrD-1 at 932.708 kb on - strand, within nqrD-1 at 932.737 kb on + strand, within nqrD-1 at 932.737 kb on + strand, within nqrD-1 at 932.745 kb on - strand, within nqrD-1 at 932.745 kb on - strand, within nqrD-1 at 932.790 kb on - strand, within nqrD-1 at 932.805 kb on - strand, within nqrD-1 at 932.805 kb on - strand, within nqrD-1 at 932.927 kb on + strand at 932.961 kb on - strand at 932.961 kb on - strand at 932.970 kb on - strand at 932.979 kb on + strand at 932.980 kb on - strand at 932.997 kb on - strand at 932.999 kb on + strand at 933.015 kb on + strand, within nqrE-1 at 933.023 kb on - strand, within nqrE-1 at 933.025 kb on + strand, within nqrE-1 at 933.035 kb on + strand, within nqrE-1 at 933.138 kb on - strand, within nqrE-1 at 933.157 kb on - strand, within nqrE-1 at 933.157 kb on - strand, within nqrE-1 at 933.157 kb on - strand, within nqrE-1 at 933.259 kb on + strand, within nqrE-1 at 933.311 kb on - strand, within nqrE-1 at 933.407 kb on - strand, within nqrE-1 at 933.407 kb on - strand, within nqrE-1 at 933.407 kb on - strand, within nqrE-1 at 933.425 kb on + strand, within nqrE-1 at 933.430 kb on - strand, within nqrE-1 at 933.468 kb on + strand, within nqrE-1 at 933.469 kb on + strand, within nqrE-1 at 933.469 kb on + strand, within nqrE-1 at 933.477 kb on - strand, within nqrE-1 at 933.486 kb on - strand, within nqrE-1 at 933.513 kb on + strand at 933.557 kb on + strand at 933.634 kb on + strand at 933.647 kb on - strand at 933.701 kb on + strand at 933.828 kb on + strand, within nqrF-1 at 934.195 kb on + strand, within nqrF-1 at 934.339 kb on + strand, within nqrF-1 at 934.364 kb on + strand, within nqrF-1 at 934.486 kb on - strand, within nqrF-1 at 934.600 kb on + strand, within nqrF-1 at 934.624 kb on - strand, within nqrF-1 at 934.631 kb on + strand, within nqrF-1 at 934.641 kb on + strand, within nqrF-1 at 934.650 kb on + strand, within nqrF-1 at 934.724 kb on + strand at 934.731 kb on + strand at 934.862 kb on - strand at 934.990 kb on - strand, within SO0908 at 935.028 kb on + strand, within SO0908 at 935.028 kb on + strand, within SO0908 at 935.036 kb on - strand, within SO0908 at 935.036 kb on - strand, within SO0908 at 935.036 kb on - strand, within SO0908 at 935.036 kb on - strand, within SO0908 at 935.192 kb on + strand, within SO0908 at 935.197 kb on + strand, within SO0908 at 935.226 kb on - strand at 935.226 kb on - strand at 935.226 kb on - strand at 935.249 kb on - strand at 935.274 kb on - strand at 935.334 kb on + strand at 935.381 kb on + strand, within SO0909 at 935.390 kb on + strand, within SO0909 at 935.390 kb on + strand, within SO0909 at 935.397 kb on + strand, within SO0909 at 935.400 kb on + strand, within SO0909 at 935.415 kb on + strand, within SO0909 at 935.523 kb on + strand, within SO0909 at 935.523 kb on + strand, within SO0909 at 935.523 kb on + strand, within SO0909 at 935.523 kb on + strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.531 kb on - strand, within SO0909 at 935.555 kb on + strand, within SO0909 at 935.577 kb on + strand, within SO0909 at 935.595 kb on - strand, within SO0909 at 935.664 kb on - strand, within SO0909 at 935.736 kb on - strand, within SO0909 at 935.774 kb on + strand, within SO0909 at 935.793 kb on + strand at 935.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 6 day remove 932,652 + nqrD-1 SO0905 0.55 -2.3 932,660 - nqrD-1 SO0905 0.56 -0.6 932,664 + nqrD-1 SO0905 0.57 -4.0 932,684 + nqrD-1 SO0905 0.60 -0.7 932,700 + nqrD-1 SO0905 0.62 -0.5 932,708 - nqrD-1 SO0905 0.64 -1.4 932,737 + nqrD-1 SO0905 0.68 -0.4 932,737 + nqrD-1 SO0905 0.68 -2.8 932,745 - nqrD-1 SO0905 0.70 -0.4 932,745 - nqrD-1 SO0905 0.70 -0.5 932,790 - nqrD-1 SO0905 0.77 -0.1 932,805 - nqrD-1 SO0905 0.79 -3.4 932,805 - nqrD-1 SO0905 0.79 -0.3 932,927 + +0.5 932,961 - -1.3 932,961 - -0.4 932,970 - +0.2 932,979 + -2.7 932,980 - -0.4 932,997 - -2.5 932,999 + -1.0 933,015 + nqrE-1 SO0906 0.12 -0.6 933,023 - nqrE-1 SO0906 0.13 -1.0 933,025 + nqrE-1 SO0906 0.13 -1.3 933,035 + nqrE-1 SO0906 0.15 -2.4 933,138 - nqrE-1 SO0906 0.32 -2.0 933,157 - nqrE-1 SO0906 0.35 -1.4 933,157 - nqrE-1 SO0906 0.35 -1.4 933,157 - nqrE-1 SO0906 0.35 -1.1 933,259 + nqrE-1 SO0906 0.52 -0.1 933,311 - nqrE-1 SO0906 0.60 -0.3 933,407 - nqrE-1 SO0906 0.76 +0.3 933,407 - nqrE-1 SO0906 0.76 +0.6 933,407 - nqrE-1 SO0906 0.76 -1.2 933,425 + nqrE-1 SO0906 0.79 -0.7 933,430 - nqrE-1 SO0906 0.80 -0.1 933,468 + nqrE-1 SO0906 0.86 -1.4 933,469 + nqrE-1 SO0906 0.86 -0.4 933,469 + nqrE-1 SO0906 0.86 -0.6 933,477 - nqrE-1 SO0906 0.88 -0.1 933,486 - nqrE-1 SO0906 0.89 -0.4 933,513 + +0.5 933,557 + +0.5 933,634 + +0.2 933,647 - -0.4 933,701 + -0.8 933,828 + nqrF-1 SO0907 0.18 -1.2 934,195 + nqrF-1 SO0907 0.49 -1.3 934,339 + nqrF-1 SO0907 0.60 -0.6 934,364 + nqrF-1 SO0907 0.62 -0.6 934,486 - nqrF-1 SO0907 0.72 -2.7 934,600 + nqrF-1 SO0907 0.82 -0.2 934,624 - nqrF-1 SO0907 0.84 -0.2 934,631 + nqrF-1 SO0907 0.84 -0.3 934,641 + nqrF-1 SO0907 0.85 +1.0 934,650 + nqrF-1 SO0907 0.86 +0.3 934,724 + -1.1 934,731 + +0.2 934,862 - -1.0 934,990 - SO0908 0.23 -0.4 935,028 + SO0908 0.35 +0.1 935,028 + SO0908 0.35 -0.7 935,036 - SO0908 0.37 -0.1 935,036 - SO0908 0.37 -0.3 935,036 - SO0908 0.37 -1.5 935,036 - SO0908 0.37 +0.2 935,192 + SO0908 0.86 -0.5 935,197 + SO0908 0.88 -0.2 935,226 - +0.3 935,226 - -1.2 935,226 - -0.1 935,249 - -0.2 935,274 - +0.7 935,334 + +0.5 935,381 + SO0909 0.18 -0.2 935,390 + SO0909 0.20 -0.5 935,390 + SO0909 0.20 +1.7 935,397 + SO0909 0.21 -0.7 935,400 + SO0909 0.22 -0.2 935,415 + SO0909 0.24 -0.5 935,523 + SO0909 0.43 -0.0 935,523 + SO0909 0.43 +0.5 935,523 + SO0909 0.43 -0.3 935,523 + SO0909 0.43 +0.0 935,531 - SO0909 0.45 +0.7 935,531 - SO0909 0.45 +0.8 935,531 - SO0909 0.45 -0.6 935,531 - SO0909 0.45 -0.2 935,531 - SO0909 0.45 -0.4 935,531 - SO0909 0.45 +0.1 935,531 - SO0909 0.45 -0.1 935,531 - SO0909 0.45 -0.5 935,531 - SO0909 0.45 +2.5 935,531 - SO0909 0.45 +0.9 935,531 - SO0909 0.45 -0.7 935,531 - SO0909 0.45 +0.7 935,555 + SO0909 0.49 +0.6 935,577 + SO0909 0.53 +0.1 935,595 - SO0909 0.56 -1.1 935,664 - SO0909 0.68 -0.5 935,736 - SO0909 0.81 -0.2 935,774 + SO0909 0.88 -0.1 935,793 + -0.4 935,801 - -0.5
Or see this region's nucleotide sequence