Experiment: Survival in PBS at 22C, experiment1, 6 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and SO0873 are separated by 274 nucleotides SO0873 and dksA are separated by 133 nucleotides dksA and sfsA are separated by 163 nucleotides
SO0872: pcnB - polyA polymerase (NCBI ptt file), at 901,508 to 902,824
pcnB
SO0873: SO0873 - glutamyl-tRNA synthetase domain protein (NCBI ptt file), at 903,099 to 903,998
SO0873
SO0874: dksA - DnaK suppressor protein (NCBI ptt file), at 904,132 to 904,575
dksA
SO0875: sfsA - sugar fermentation stimulation protein (NCBI ptt file), at 904,739 to 905,443
sfsA
Position (kb) 903
904 Strain fitness (log2 ratio) -1
0
1
2
3 at 902.288 kb on - strand, within pcnB at 902.288 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.358 kb on - strand, within pcnB at 902.714 kb on - strand at 902.744 kb on - strand at 902.750 kb on + strand at 902.833 kb on - strand at 902.868 kb on - strand at 902.896 kb on - strand at 903.055 kb on - strand at 903.055 kb on - strand at 903.134 kb on - strand at 903.207 kb on + strand, within SO0873 at 903.216 kb on + strand, within SO0873 at 903.227 kb on + strand, within SO0873 at 903.438 kb on - strand, within SO0873 at 903.467 kb on + strand, within SO0873 at 903.591 kb on + strand, within SO0873 at 903.721 kb on + strand, within SO0873 at 903.751 kb on + strand, within SO0873 at 903.768 kb on + strand, within SO0873 at 903.784 kb on - strand, within SO0873 at 903.845 kb on + strand, within SO0873 at 903.853 kb on - strand, within SO0873 at 903.953 kb on + strand at 904.061 kb on + strand at 904.071 kb on - strand at 904.075 kb on + strand at 904.366 kb on - strand, within dksA at 904.421 kb on - strand, within dksA at 904.702 kb on - strand at 904.756 kb on - strand at 904.763 kb on + strand at 904.763 kb on + strand at 904.771 kb on - strand at 904.771 kb on - strand at 904.930 kb on + strand, within sfsA at 904.938 kb on - strand, within sfsA at 904.997 kb on + strand, within sfsA
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 6 day remove 902,288 - pcnB SO0872 0.59 -1.0 902,288 - pcnB SO0872 0.59 -0.3 902,343 - pcnB SO0872 0.63 +0.3 902,343 - pcnB SO0872 0.63 -0.5 902,343 - pcnB SO0872 0.63 +1.5 902,343 - pcnB SO0872 0.63 -0.5 902,343 - pcnB SO0872 0.63 -1.0 902,343 - pcnB SO0872 0.63 +0.0 902,343 - pcnB SO0872 0.63 +1.0 902,343 - pcnB SO0872 0.63 -0.3 902,343 - pcnB SO0872 0.63 +0.0 902,343 - pcnB SO0872 0.63 +2.6 902,358 - pcnB SO0872 0.65 -0.8 902,714 - +0.6 902,744 - -0.5 902,750 + +1.8 902,833 - +1.2 902,868 - -0.3 902,896 - +2.2 903,055 - -0.5 903,055 - +1.7 903,134 - +0.7 903,207 + SO0873 0.12 -0.3 903,216 + SO0873 0.13 -0.2 903,227 + SO0873 0.14 +1.0 903,438 - SO0873 0.38 +1.6 903,467 + SO0873 0.41 -0.7 903,591 + SO0873 0.55 -1.0 903,721 + SO0873 0.69 +0.3 903,751 + SO0873 0.72 -0.4 903,768 + SO0873 0.74 +0.8 903,784 - SO0873 0.76 -0.5 903,845 + SO0873 0.83 -1.4 903,853 - SO0873 0.84 +3.0 903,953 + +1.0 904,061 + -0.2 904,071 - -0.0 904,075 + +0.2 904,366 - dksA SO0874 0.53 +1.0 904,421 - dksA SO0874 0.65 +1.0 904,702 - +0.2 904,756 - -1.0 904,763 + +0.2 904,763 + +0.2 904,771 - -0.0 904,771 - +0.2 904,930 + sfsA SO0875 0.27 -0.4 904,938 - sfsA SO0875 0.28 +0.2 904,997 + sfsA SO0875 0.37 -0.3
Or see this region's nucleotide sequence