Experiment: Survival in PBS at 22C, experiment1, 2 day
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1921 and SO1922 are separated by 159 nucleotides SO1922 and SO1923 are separated by 56 nucleotides SO1923 and SO1924 overlap by 4 nucleotides
SO1921: SO1921 - hypothetical protein (NCBI ptt file), at 2,020,745 to 2,020,948
SO1921
SO1922: SO1922 - conserved hypothetical protein (NCBI ptt file), at 2,021,108 to 2,021,422
SO1922
SO1923: SO1923 - AcrB/AcrD/AcrF family protein (NCBI ptt file), at 2,021,479 to 2,024,541
SO1923
SO1924: SO1924 - AcrB/AcrD/AcrF family protein (NCBI ptt file), at 2,024,538 to 2,027,813
SO1924
Position (kb)
2021
2022
2023
2024
2025 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2020.864 kb on + strand, within SO1921 at 2020.864 kb on + strand, within SO1921 at 2020.872 kb on - strand, within SO1921 at 2020.892 kb on - strand, within SO1921 at 2021.100 kb on + strand at 2021.283 kb on + strand, within SO1922 at 2021.291 kb on - strand, within SO1922 at 2021.301 kb on - strand, within SO1922 at 2021.301 kb on - strand, within SO1922 at 2021.384 kb on - strand, within SO1922 at 2021.384 kb on - strand, within SO1922 at 2021.450 kb on - strand at 2021.461 kb on + strand at 2021.463 kb on - strand at 2021.532 kb on + strand at 2021.575 kb on + strand at 2021.599 kb on + strand at 2021.704 kb on + strand at 2021.704 kb on + strand at 2021.704 kb on + strand at 2021.704 kb on + strand at 2021.712 kb on - strand at 2021.712 kb on - strand at 2021.712 kb on - strand at 2021.749 kb on + strand at 2021.752 kb on + strand at 2021.752 kb on + strand at 2021.760 kb on + strand at 2021.782 kb on - strand at 2021.831 kb on - strand, within SO1923 at 2021.849 kb on - strand, within SO1923 at 2021.940 kb on + strand, within SO1923 at 2022.199 kb on - strand, within SO1923 at 2022.208 kb on - strand, within SO1923 at 2022.297 kb on + strand, within SO1923 at 2022.304 kb on - strand, within SO1923 at 2022.305 kb on - strand, within SO1923 at 2022.487 kb on + strand, within SO1923 at 2022.555 kb on - strand, within SO1923 at 2022.570 kb on + strand, within SO1923 at 2022.666 kb on - strand, within SO1923 at 2022.678 kb on - strand, within SO1923 at 2022.771 kb on - strand, within SO1923 at 2022.771 kb on - strand, within SO1923 at 2022.771 kb on - strand, within SO1923 at 2022.874 kb on - strand, within SO1923 at 2022.994 kb on - strand, within SO1923 at 2023.008 kb on + strand, within SO1923 at 2023.176 kb on - strand, within SO1923 at 2023.244 kb on - strand, within SO1923 at 2023.312 kb on - strand, within SO1923 at 2023.331 kb on + strand, within SO1923 at 2023.362 kb on - strand, within SO1923 at 2023.437 kb on + strand, within SO1923 at 2023.634 kb on + strand, within SO1923 at 2023.698 kb on + strand, within SO1923 at 2023.721 kb on + strand, within SO1923 at 2023.721 kb on + strand, within SO1923 at 2023.729 kb on - strand, within SO1923 at 2023.729 kb on - strand, within SO1923 at 2023.729 kb on - strand, within SO1923 at 2023.743 kb on + strand, within SO1923 at 2023.846 kb on - strand, within SO1923 at 2023.884 kb on + strand, within SO1923 at 2023.984 kb on - strand, within SO1923 at 2024.009 kb on + strand, within SO1923 at 2024.012 kb on + strand, within SO1923 at 2024.025 kb on + strand, within SO1923 at 2024.033 kb on - strand, within SO1923 at 2024.033 kb on - strand, within SO1923 at 2024.220 kb on + strand, within SO1923 at 2024.272 kb on + strand at 2024.305 kb on - strand at 2024.307 kb on + strand at 2024.315 kb on - strand at 2024.315 kb on - strand at 2024.376 kb on + strand at 2024.427 kb on - strand at 2024.637 kb on - strand at 2024.644 kb on - strand at 2024.714 kb on + strand at 2024.754 kb on + strand at 2024.762 kb on - strand at 2024.762 kb on - strand at 2024.762 kb on - strand at 2024.762 kb on - strand at 2024.807 kb on - strand at 2024.855 kb on + strand at 2024.913 kb on - strand, within SO1924 at 2025.055 kb on + strand, within SO1924 at 2025.063 kb on - strand, within SO1924 at 2025.135 kb on + strand, within SO1924 at 2025.292 kb on - strand, within SO1924
Per-strain Table
Position Strand Gene LocusTag Fraction Survival in PBS at 22C, experiment1, 2 day remove 2,020,864 + SO1921 0.58 -1.1 2,020,864 + SO1921 0.58 +0.3 2,020,872 - SO1921 0.62 -0.5 2,020,892 - SO1921 0.72 +0.4 2,021,100 + -0.5 2,021,283 + SO1922 0.56 +0.3 2,021,291 - SO1922 0.58 -0.2 2,021,301 - SO1922 0.61 +0.1 2,021,301 - SO1922 0.61 +0.2 2,021,384 - SO1922 0.88 -0.3 2,021,384 - SO1922 0.88 -0.5 2,021,450 - +0.9 2,021,461 + +1.5 2,021,463 - -0.1 2,021,532 + +0.6 2,021,575 + -1.6 2,021,599 + +0.2 2,021,704 + +0.4 2,021,704 + -0.6 2,021,704 + -0.5 2,021,704 + -1.6 2,021,712 - +1.8 2,021,712 - +0.1 2,021,712 - -0.5 2,021,749 + -1.0 2,021,752 + -0.3 2,021,752 + -0.8 2,021,760 + +0.5 2,021,782 - -0.2 2,021,831 - SO1923 0.11 +0.1 2,021,849 - SO1923 0.12 -1.8 2,021,940 + SO1923 0.15 +0.6 2,022,199 - SO1923 0.24 +0.4 2,022,208 - SO1923 0.24 +1.2 2,022,297 + SO1923 0.27 +0.1 2,022,304 - SO1923 0.27 +0.4 2,022,305 - SO1923 0.27 +0.2 2,022,487 + SO1923 0.33 +0.2 2,022,555 - SO1923 0.35 -0.0 2,022,570 + SO1923 0.36 +0.5 2,022,666 - SO1923 0.39 -0.7 2,022,678 - SO1923 0.39 +0.3 2,022,771 - SO1923 0.42 +1.3 2,022,771 - SO1923 0.42 -0.2 2,022,771 - SO1923 0.42 -1.1 2,022,874 - SO1923 0.46 +0.3 2,022,994 - SO1923 0.49 -0.6 2,023,008 + SO1923 0.50 -0.3 2,023,176 - SO1923 0.55 -0.1 2,023,244 - SO1923 0.58 +1.2 2,023,312 - SO1923 0.60 +1.2 2,023,331 + SO1923 0.60 +0.2 2,023,362 - SO1923 0.61 -0.8 2,023,437 + SO1923 0.64 +0.4 2,023,634 + SO1923 0.70 +0.5 2,023,698 + SO1923 0.72 +0.5 2,023,721 + SO1923 0.73 +0.2 2,023,721 + SO1923 0.73 -0.5 2,023,729 - SO1923 0.73 +0.4 2,023,729 - SO1923 0.73 +0.1 2,023,729 - SO1923 0.73 +0.3 2,023,743 + SO1923 0.74 +0.0 2,023,846 - SO1923 0.77 -1.3 2,023,884 + SO1923 0.79 +0.0 2,023,984 - SO1923 0.82 +0.2 2,024,009 + SO1923 0.83 -0.1 2,024,012 + SO1923 0.83 -1.1 2,024,025 + SO1923 0.83 +0.1 2,024,033 - SO1923 0.83 -0.3 2,024,033 - SO1923 0.83 +0.6 2,024,220 + SO1923 0.89 +0.2 2,024,272 + +0.4 2,024,305 - -0.3 2,024,307 + +0.2 2,024,315 - +1.4 2,024,315 - -0.2 2,024,376 + -0.0 2,024,427 - -0.8 2,024,637 - +0.3 2,024,644 - -0.1 2,024,714 + +0.4 2,024,754 + +0.9 2,024,762 - -0.0 2,024,762 - -2.0 2,024,762 - -1.8 2,024,762 - -1.0 2,024,807 - +0.3 2,024,855 + -1.7 2,024,913 - SO1924 0.11 +1.0 2,025,055 + SO1924 0.16 -0.2 2,025,063 - SO1924 0.16 -0.7 2,025,135 + SO1924 0.18 -0.4 2,025,292 - SO1924 0.23 +0.2
Or see this region's nucleotide sequence