Strain Fitness in Paraburkholderia graminis OAS925 around ABIE53_000573

Experiment: RCH2_defined_glucose_5mM with 2-Deoxy-D-glucose 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABIE53_000570 and ABIE53_000571 overlap by 4 nucleotidesABIE53_000571 and ABIE53_000572 are separated by 5 nucleotidesABIE53_000572 and ABIE53_000573 are separated by 62 nucleotidesABIE53_000573 and ABIE53_000574 are separated by 36 nucleotides ABIE53_000570: ABIE53_000570 - acyl dehydratase, at 622,103 to 622,576 _000570 ABIE53_000571: ABIE53_000571 - putative Fe-S protein YdhL (DUF1289 family), at 622,573 to 622,800 _000571 ABIE53_000572: ABIE53_000572 - prolyl-tRNA editing enzyme YbaK/EbsC (Cys-tRNA(Pro) deacylase), at 622,806 to 623,327 _000572 ABIE53_000573: ABIE53_000573 - hydroxymethylglutaryl-CoA lyase, at 623,390 to 624,316 _000573 ABIE53_000574: ABIE53_000574 - glyoxylate/hydroxypyruvate reductase A, at 624,353 to 625,294 _000574 Position (kb) 623 624 625Strain fitness (log2 ratio) -1 0 1 2 3at 622.446 kb on + strand, within ABIE53_000570at 622.447 kb on - strand, within ABIE53_000570at 622.534 kb on + strandat 622.661 kb on + strand, within ABIE53_000571at 622.661 kb on + strand, within ABIE53_000571at 622.661 kb on + strand, within ABIE53_000571at 623.353 kb on - strandat 623.393 kb on + strandat 623.393 kb on + strandat 623.547 kb on + strand, within ABIE53_000573at 623.547 kb on + strand, within ABIE53_000573at 623.547 kb on + strand, within ABIE53_000573at 623.548 kb on - strand, within ABIE53_000573at 623.548 kb on - strand, within ABIE53_000573at 623.628 kb on + strand, within ABIE53_000573at 623.628 kb on + strand, within ABIE53_000573at 623.629 kb on - strand, within ABIE53_000573at 623.658 kb on + strand, within ABIE53_000573at 623.658 kb on + strand, within ABIE53_000573at 623.658 kb on + strand, within ABIE53_000573at 623.658 kb on + strand, within ABIE53_000573at 623.658 kb on + strand, within ABIE53_000573at 623.659 kb on - strand, within ABIE53_000573at 623.659 kb on - strand, within ABIE53_000573at 623.662 kb on + strand, within ABIE53_000573at 623.663 kb on - strand, within ABIE53_000573at 623.663 kb on - strand, within ABIE53_000573at 623.682 kb on + strand, within ABIE53_000573at 623.682 kb on + strand, within ABIE53_000573at 623.682 kb on + strand, within ABIE53_000573at 623.682 kb on + strand, within ABIE53_000573at 623.683 kb on - strand, within ABIE53_000573at 623.782 kb on + strand, within ABIE53_000573at 623.783 kb on - strand, within ABIE53_000573at 623.868 kb on + strand, within ABIE53_000573at 623.869 kb on - strand, within ABIE53_000573at 624.088 kb on - strand, within ABIE53_000573at 624.088 kb on - strand, within ABIE53_000573at 624.088 kb on - strand, within ABIE53_000573at 624.481 kb on + strand, within ABIE53_000574at 624.481 kb on + strand, within ABIE53_000574at 624.482 kb on - strand, within ABIE53_000574at 624.630 kb on + strand, within ABIE53_000574at 624.630 kb on + strand, within ABIE53_000574at 624.631 kb on - strand, within ABIE53_000574at 624.691 kb on + strand, within ABIE53_000574at 624.942 kb on + strand, within ABIE53_000574at 624.963 kb on + strand, within ABIE53_000574at 624.963 kb on + strand, within ABIE53_000574at 624.963 kb on + strand, within ABIE53_000574at 624.964 kb on - strand, within ABIE53_000574at 624.987 kb on + strand, within ABIE53_000574at 624.987 kb on + strand, within ABIE53_000574at 624.987 kb on + strand, within ABIE53_000574at 624.987 kb on + strand, within ABIE53_000574at 624.988 kb on - strand, within ABIE53_000574at 624.988 kb on - strand, within ABIE53_000574at 624.988 kb on - strand, within ABIE53_000574at 625.277 kb on - strandat 625.277 kb on - strandat 625.277 kb on - strandat 625.277 kb on - strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.278 kb on + strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strandat 625.279 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with 2-Deoxy-D-glucose 5 mM
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622,446 + ABIE53_000570 0.72 +2.2
622,447 - ABIE53_000570 0.73 +1.7
622,534 + -1.5
622,661 + ABIE53_000571 0.39 +1.7
622,661 + ABIE53_000571 0.39 +0.3
622,661 + ABIE53_000571 0.39 +2.0
623,353 - -0.3
623,393 + +0.2
623,393 + -0.3
623,547 + ABIE53_000573 0.17 +2.3
623,547 + ABIE53_000573 0.17 +2.7
623,547 + ABIE53_000573 0.17 -1.0
623,548 - ABIE53_000573 0.17 +2.0
623,548 - ABIE53_000573 0.17 +0.6
623,628 + ABIE53_000573 0.26 -1.6
623,628 + ABIE53_000573 0.26 +0.3
623,629 - ABIE53_000573 0.26 -0.9
623,658 + ABIE53_000573 0.29 +0.1
623,658 + ABIE53_000573 0.29 +1.0
623,658 + ABIE53_000573 0.29 -1.2
623,658 + ABIE53_000573 0.29 -0.6
623,658 + ABIE53_000573 0.29 -0.9
623,659 - ABIE53_000573 0.29 +0.1
623,659 - ABIE53_000573 0.29 -0.3
623,662 + ABIE53_000573 0.29 +0.1
623,663 - ABIE53_000573 0.29 +2.3
623,663 - ABIE53_000573 0.29 +1.3
623,682 + ABIE53_000573 0.31 +1.3
623,682 + ABIE53_000573 0.31 -0.9
623,682 + ABIE53_000573 0.31 -0.3
623,682 + ABIE53_000573 0.31 +0.2
623,683 - ABIE53_000573 0.32 -1.3
623,782 + ABIE53_000573 0.42 -0.4
623,783 - ABIE53_000573 0.42 +1.7
623,868 + ABIE53_000573 0.52 +3.3
623,869 - ABIE53_000573 0.52 -1.3
624,088 - ABIE53_000573 0.75 -1.1
624,088 - ABIE53_000573 0.75 +2.7
624,088 - ABIE53_000573 0.75 -0.6
624,481 + ABIE53_000574 0.14 +2.1
624,481 + ABIE53_000574 0.14 -0.9
624,482 - ABIE53_000574 0.14 -1.6
624,630 + ABIE53_000574 0.29 +0.3
624,630 + ABIE53_000574 0.29 +1.3
624,631 - ABIE53_000574 0.30 -1.0
624,691 + ABIE53_000574 0.36 -0.7
624,942 + ABIE53_000574 0.63 -0.7
624,963 + ABIE53_000574 0.65 -0.8
624,963 + ABIE53_000574 0.65 +0.7
624,963 + ABIE53_000574 0.65 -0.6
624,964 - ABIE53_000574 0.65 +0.0
624,987 + ABIE53_000574 0.67 -0.3
624,987 + ABIE53_000574 0.67 -0.4
624,987 + ABIE53_000574 0.67 -0.4
624,987 + ABIE53_000574 0.67 +0.4
624,988 - ABIE53_000574 0.67 -1.4
624,988 - ABIE53_000574 0.67 +0.3
624,988 - ABIE53_000574 0.67 -1.1
625,277 - -0.4
625,277 - +0.5
625,277 - -0.0
625,277 - -0.7
625,278 + +0.1
625,278 + -0.7
625,278 + -0.4
625,278 + +0.4
625,278 + -0.9
625,278 + +0.7
625,278 + -1.1
625,278 + +0.2
625,278 + +0.1
625,278 + +0.5
625,278 + -0.5
625,278 + -0.3
625,278 + -0.0
625,279 - -0.1
625,279 - -0.3
625,279 - +0.8
625,279 - -0.5
625,279 - -0.5
625,279 - -0.6
625,279 - +0.5
625,279 - +0.7
625,279 - -0.9
625,279 - -1.7
625,279 - +0.7
625,279 - -0.3
625,279 - +1.7
625,279 - -0.3
625,279 - +1.1

Or see this region's nucleotide sequence