Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22715

Experiment: D-Glucose 80 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22715 and BWI76_RS22720 overlap by 1 nucleotidesBWI76_RS22720 and BWI76_RS22725 overlap by 4 nucleotides BWI76_RS22715: BWI76_RS22715 - type I-E CRISPR-associated endoribonuclease Cas2, at 4,734,507 to 4,734,800 _RS22715 BWI76_RS22720: BWI76_RS22720 - subtype I-E CRISPR-associated endonuclease Cas1, at 4,734,800 to 4,735,720 _RS22720 BWI76_RS22725: BWI76_RS22725 - type I-E CRISPR-associated protein Cas6/Cse3/CasE, at 4,735,717 to 4,736,367 _RS22725 Position (kb) 4734 4735Strain fitness (log2 ratio) -2 -1 0 1at 4733.537 kb on - strandat 4733.553 kb on + strandat 4733.553 kb on + strandat 4733.553 kb on + strandat 4733.561 kb on - strandat 4733.561 kb on - strandat 4733.561 kb on - strandat 4733.561 kb on - strandat 4733.939 kb on + strandat 4733.976 kb on + strandat 4734.083 kb on + strandat 4734.254 kb on + strandat 4734.286 kb on + strandat 4734.286 kb on + strandat 4734.315 kb on - strandat 4734.467 kb on + strandat 4734.565 kb on - strand, within BWI76_RS22715at 4734.565 kb on - strand, within BWI76_RS22715at 4734.619 kb on + strand, within BWI76_RS22715at 4734.632 kb on - strand, within BWI76_RS22715at 4734.632 kb on - strand, within BWI76_RS22715at 4734.632 kb on - strand, within BWI76_RS22715at 4734.876 kb on + strandat 4734.876 kb on + strandat 4734.886 kb on + strandat 4734.943 kb on + strand, within BWI76_RS22720at 4734.943 kb on + strand, within BWI76_RS22720at 4734.943 kb on + strand, within BWI76_RS22720at 4734.951 kb on - strand, within BWI76_RS22720at 4734.951 kb on - strand, within BWI76_RS22720at 4734.951 kb on - strand, within BWI76_RS22720at 4734.951 kb on - strand, within BWI76_RS22720at 4735.143 kb on + strand, within BWI76_RS22720at 4735.170 kb on - strand, within BWI76_RS22720at 4735.189 kb on + strand, within BWI76_RS22720at 4735.214 kb on + strand, within BWI76_RS22720at 4735.243 kb on + strand, within BWI76_RS22720at 4735.251 kb on - strand, within BWI76_RS22720at 4735.263 kb on + strand, within BWI76_RS22720at 4735.297 kb on + strand, within BWI76_RS22720at 4735.297 kb on + strand, within BWI76_RS22720at 4735.305 kb on - strand, within BWI76_RS22720at 4735.305 kb on - strand, within BWI76_RS22720at 4735.305 kb on - strand, within BWI76_RS22720at 4735.335 kb on + strand, within BWI76_RS22720at 4735.708 kb on + strandat 4735.708 kb on + strandat 4735.751 kb on - strandat 4735.751 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose 80 mM (C)
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4,733,537 - -1.1
4,733,553 + +0.2
4,733,553 + -0.4
4,733,553 + -0.4
4,733,561 - -0.2
4,733,561 - +0.2
4,733,561 - -1.2
4,733,561 - +0.1
4,733,939 + +0.1
4,733,976 + +0.3
4,734,083 + +0.9
4,734,254 + -0.8
4,734,286 + +0.4
4,734,286 + -0.0
4,734,315 - +0.5
4,734,467 + -0.1
4,734,565 - BWI76_RS22715 0.20 -0.9
4,734,565 - BWI76_RS22715 0.20 -2.0
4,734,619 + BWI76_RS22715 0.38 -0.4
4,734,632 - BWI76_RS22715 0.43 -1.2
4,734,632 - BWI76_RS22715 0.43 -0.5
4,734,632 - BWI76_RS22715 0.43 -0.2
4,734,876 + -0.1
4,734,876 + -0.2
4,734,886 + -0.4
4,734,943 + BWI76_RS22720 0.16 -0.2
4,734,943 + BWI76_RS22720 0.16 +0.4
4,734,943 + BWI76_RS22720 0.16 -0.6
4,734,951 - BWI76_RS22720 0.16 -0.2
4,734,951 - BWI76_RS22720 0.16 -0.7
4,734,951 - BWI76_RS22720 0.16 +0.1
4,734,951 - BWI76_RS22720 0.16 +0.3
4,735,143 + BWI76_RS22720 0.37 +0.1
4,735,170 - BWI76_RS22720 0.40 +0.5
4,735,189 + BWI76_RS22720 0.42 +0.2
4,735,214 + BWI76_RS22720 0.45 -0.0
4,735,243 + BWI76_RS22720 0.48 -0.0
4,735,251 - BWI76_RS22720 0.49 -1.0
4,735,263 + BWI76_RS22720 0.50 -0.4
4,735,297 + BWI76_RS22720 0.54 -1.5
4,735,297 + BWI76_RS22720 0.54 -0.7
4,735,305 - BWI76_RS22720 0.55 -1.1
4,735,305 - BWI76_RS22720 0.55 -0.3
4,735,305 - BWI76_RS22720 0.55 -0.0
4,735,335 + BWI76_RS22720 0.58 -0.9
4,735,708 + +0.2
4,735,708 + -0.1
4,735,751 - -0.1
4,735,751 - +0.4

Or see this region's nucleotide sequence