Experiment: RCH2_defined_glucose_5mM with 2,3-dihydroxybenzoic 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_003375 and ABIE53_003376 are separated by 248 nucleotides ABIE53_003376 and ABIE53_003377 are separated by 257 nucleotides ABIE53_003377 and ABIE53_003378 are separated by 63 nucleotides
ABIE53_003375: ABIE53_003375 - uncharacterized protein YecE (DUF72 family), at 3,797,162 to 3,798,514
_003375
ABIE53_003376: ABIE53_003376 - peptide-methionine (S)-S-oxide reductase, at 3,798,763 to 3,799,311
_003376
ABIE53_003377: ABIE53_003377 - flavin reductase (DIM6/NTAB) family NADH-FMN oxidoreductase RutF, at 3,799,569 to 3,800,078
_003377
ABIE53_003378: ABIE53_003378 - Lrp/AsnC family leucine-responsive transcriptional regulator, at 3,800,142 to 3,800,657
_003378
Position (kb)
3798
3799
3800 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3797.792 kb on + strand, within ABIE53_003375 at 3797.793 kb on - strand, within ABIE53_003375 at 3797.793 kb on - strand, within ABIE53_003375 at 3797.793 kb on - strand, within ABIE53_003375 at 3797.816 kb on + strand, within ABIE53_003375 at 3797.816 kb on + strand, within ABIE53_003375 at 3797.816 kb on + strand, within ABIE53_003375 at 3797.817 kb on - strand, within ABIE53_003375 at 3797.825 kb on + strand, within ABIE53_003375 at 3797.825 kb on + strand, within ABIE53_003375 at 3797.825 kb on + strand, within ABIE53_003375 at 3797.826 kb on - strand, within ABIE53_003375 at 3798.125 kb on + strand, within ABIE53_003375 at 3798.125 kb on + strand, within ABIE53_003375 at 3798.126 kb on - strand, within ABIE53_003375 at 3798.126 kb on - strand, within ABIE53_003375 at 3798.126 kb on - strand, within ABIE53_003375 at 3798.126 kb on - strand, within ABIE53_003375 at 3798.126 kb on - strand, within ABIE53_003375 at 3798.126 kb on - strand, within ABIE53_003375 at 3798.126 kb on - strand, within ABIE53_003375 at 3798.175 kb on + strand, within ABIE53_003375 at 3798.176 kb on - strand, within ABIE53_003375 at 3798.200 kb on + strand, within ABIE53_003375 at 3798.261 kb on + strand, within ABIE53_003375 at 3798.262 kb on - strand, within ABIE53_003375 at 3798.262 kb on - strand, within ABIE53_003375 at 3798.323 kb on + strand, within ABIE53_003375 at 3798.323 kb on + strand, within ABIE53_003375 at 3798.323 kb on + strand, within ABIE53_003375 at 3798.324 kb on - strand, within ABIE53_003375 at 3798.324 kb on - strand, within ABIE53_003375 at 3798.324 kb on - strand, within ABIE53_003375 at 3798.345 kb on - strand, within ABIE53_003375 at 3798.345 kb on - strand, within ABIE53_003375 at 3798.345 kb on - strand, within ABIE53_003375 at 3798.358 kb on + strand, within ABIE53_003375 at 3798.358 kb on + strand, within ABIE53_003375 at 3798.358 kb on + strand, within ABIE53_003375 at 3798.359 kb on - strand, within ABIE53_003375 at 3798.406 kb on - strand at 3798.406 kb on - strand at 3798.406 kb on - strand at 3798.416 kb on + strand at 3798.455 kb on - strand at 3798.455 kb on - strand at 3798.455 kb on - strand at 3798.519 kb on + strand at 3798.519 kb on + strand at 3798.519 kb on + strand at 3798.519 kb on + strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.520 kb on - strand at 3798.836 kb on + strand, within ABIE53_003376 at 3798.836 kb on + strand, within ABIE53_003376 at 3798.836 kb on + strand, within ABIE53_003376 at 3798.836 kb on + strand, within ABIE53_003376 at 3798.836 kb on + strand, within ABIE53_003376 at 3798.836 kb on + strand, within ABIE53_003376 at 3798.837 kb on - strand, within ABIE53_003376 at 3798.837 kb on - strand, within ABIE53_003376 at 3798.837 kb on - strand, within ABIE53_003376 at 3798.837 kb on - strand, within ABIE53_003376 at 3798.837 kb on - strand, within ABIE53_003376 at 3798.837 kb on - strand, within ABIE53_003376 at 3798.837 kb on - strand, within ABIE53_003376 at 3798.863 kb on + strand, within ABIE53_003376 at 3798.864 kb on - strand, within ABIE53_003376 at 3798.864 kb on - strand, within ABIE53_003376 at 3798.864 kb on - strand, within ABIE53_003376 at 3798.875 kb on + strand, within ABIE53_003376 at 3798.875 kb on + strand, within ABIE53_003376 at 3798.875 kb on + strand, within ABIE53_003376 at 3798.875 kb on + strand, within ABIE53_003376 at 3798.875 kb on + strand, within ABIE53_003376 at 3798.875 kb on + strand, within ABIE53_003376 at 3798.876 kb on - strand, within ABIE53_003376 at 3798.876 kb on - strand, within ABIE53_003376 at 3798.876 kb on - strand, within ABIE53_003376 at 3798.893 kb on + strand, within ABIE53_003376 at 3798.893 kb on + strand, within ABIE53_003376 at 3798.893 kb on + strand, within ABIE53_003376 at 3798.893 kb on + strand, within ABIE53_003376 at 3798.894 kb on - strand, within ABIE53_003376 at 3798.894 kb on - strand, within ABIE53_003376 at 3798.894 kb on - strand, within ABIE53_003376 at 3798.894 kb on - strand, within ABIE53_003376 at 3798.894 kb on - strand, within ABIE53_003376 at 3799.023 kb on - strand, within ABIE53_003376 at 3799.023 kb on - strand, within ABIE53_003376 at 3799.041 kb on + strand, within ABIE53_003376 at 3799.042 kb on - strand, within ABIE53_003376 at 3799.042 kb on - strand, within ABIE53_003376 at 3799.042 kb on - strand, within ABIE53_003376 at 3799.042 kb on - strand, within ABIE53_003376 at 3799.042 kb on - strand, within ABIE53_003376 at 3799.103 kb on + strand, within ABIE53_003376 at 3799.103 kb on + strand, within ABIE53_003376 at 3799.103 kb on + strand, within ABIE53_003376 at 3799.104 kb on - strand, within ABIE53_003376 at 3799.104 kb on - strand, within ABIE53_003376 at 3799.178 kb on + strand, within ABIE53_003376 at 3799.178 kb on + strand, within ABIE53_003376 at 3799.178 kb on + strand, within ABIE53_003376 at 3799.178 kb on + strand, within ABIE53_003376 at 3799.178 kb on + strand, within ABIE53_003376 at 3799.179 kb on - strand, within ABIE53_003376 at 3799.179 kb on - strand, within ABIE53_003376 at 3799.179 kb on - strand, within ABIE53_003376 at 3799.179 kb on - strand, within ABIE53_003376 at 3799.247 kb on + strand, within ABIE53_003376 at 3799.248 kb on - strand, within ABIE53_003376 at 3799.248 kb on - strand, within ABIE53_003376 at 3799.248 kb on - strand, within ABIE53_003376 at 3799.385 kb on + strand at 3799.385 kb on + strand at 3799.399 kb on - strand at 3799.454 kb on - strand at 3799.454 kb on - strand at 3799.482 kb on + strand at 3799.482 kb on + strand at 3799.483 kb on - strand at 3799.483 kb on - strand at 3799.791 kb on + strand, within ABIE53_003377 at 3799.791 kb on + strand, within ABIE53_003377 at 3799.791 kb on + strand, within ABIE53_003377 at 3799.791 kb on + strand, within ABIE53_003377 at 3799.791 kb on + strand, within ABIE53_003377 at 3799.791 kb on + strand, within ABIE53_003377 at 3799.792 kb on - strand, within ABIE53_003377 at 3799.792 kb on - strand, within ABIE53_003377 at 3799.792 kb on - strand, within ABIE53_003377 at 3799.792 kb on - strand, within ABIE53_003377 at 3799.792 kb on - strand, within ABIE53_003377 at 3799.792 kb on - strand, within ABIE53_003377 at 3799.792 kb on - strand, within ABIE53_003377 at 3799.792 kb on - strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.828 kb on + strand, within ABIE53_003377 at 3799.829 kb on - strand, within ABIE53_003377 at 3799.829 kb on - strand, within ABIE53_003377 at 3799.829 kb on - strand, within ABIE53_003377 at 3799.829 kb on - strand, within ABIE53_003377 at 3799.829 kb on - strand, within ABIE53_003377 at 3799.829 kb on - strand, within ABIE53_003377 at 3799.829 kb on - strand, within ABIE53_003377 at 3799.873 kb on + strand, within ABIE53_003377 at 3799.874 kb on - strand, within ABIE53_003377 at 3799.874 kb on - strand, within ABIE53_003377 at 3799.898 kb on + strand, within ABIE53_003377 at 3799.940 kb on + strand, within ABIE53_003377 at 3799.953 kb on + strand, within ABIE53_003377 at 3800.076 kb on + strand at 3800.076 kb on + strand at 3800.077 kb on - strand at 3800.077 kb on - strand at 3800.077 kb on - strand at 3800.077 kb on - strand at 3800.077 kb on - strand at 3800.077 kb on - strand at 3800.077 kb on - strand at 3800.077 kb on - strand at 3800.234 kb on + strand, within ABIE53_003378 at 3800.234 kb on + strand, within ABIE53_003378 at 3800.235 kb on - strand, within ABIE53_003378 at 3800.235 kb on - strand, within ABIE53_003378 at 3800.246 kb on + strand, within ABIE53_003378 at 3800.247 kb on - strand, within ABIE53_003378 at 3800.267 kb on + strand, within ABIE53_003378 at 3800.267 kb on + strand, within ABIE53_003378 at 3800.267 kb on + strand, within ABIE53_003378 at 3800.267 kb on + strand, within ABIE53_003378 at 3800.267 kb on + strand, within ABIE53_003378 at 3800.268 kb on - strand, within ABIE53_003378 at 3800.268 kb on - strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.269 kb on + strand, within ABIE53_003378 at 3800.270 kb on - strand, within ABIE53_003378 at 3800.270 kb on - strand, within ABIE53_003378 at 3800.270 kb on - strand, within ABIE53_003378 at 3800.270 kb on - strand, within ABIE53_003378 at 3800.296 kb on + strand, within ABIE53_003378 at 3800.296 kb on + strand, within ABIE53_003378 at 3800.296 kb on + strand, within ABIE53_003378 at 3800.296 kb on + strand, within ABIE53_003378 at 3800.296 kb on + strand, within ABIE53_003378
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose_5mM with 2,3-dihydroxybenzoic 5 mM remove 3,797,792 + ABIE53_003375 0.47 +0.0 3,797,793 - ABIE53_003375 0.47 +0.6 3,797,793 - ABIE53_003375 0.47 +0.6 3,797,793 - ABIE53_003375 0.47 -0.5 3,797,816 + ABIE53_003375 0.48 -0.9 3,797,816 + ABIE53_003375 0.48 +0.6 3,797,816 + ABIE53_003375 0.48 +0.6 3,797,817 - ABIE53_003375 0.48 +0.2 3,797,825 + ABIE53_003375 0.49 -0.4 3,797,825 + ABIE53_003375 0.49 +0.6 3,797,825 + ABIE53_003375 0.49 -0.1 3,797,826 - ABIE53_003375 0.49 +1.6 3,798,125 + ABIE53_003375 0.71 +0.3 3,798,125 + ABIE53_003375 0.71 -0.4 3,798,126 - ABIE53_003375 0.71 +1.5 3,798,126 - ABIE53_003375 0.71 +1.5 3,798,126 - ABIE53_003375 0.71 +1.2 3,798,126 - ABIE53_003375 0.71 +1.4 3,798,126 - ABIE53_003375 0.71 +1.2 3,798,126 - ABIE53_003375 0.71 +0.6 3,798,126 - ABIE53_003375 0.71 +1.3 3,798,175 + ABIE53_003375 0.75 +3.2 3,798,176 - ABIE53_003375 0.75 -0.3 3,798,200 + ABIE53_003375 0.77 -1.2 3,798,261 + ABIE53_003375 0.81 +3.6 3,798,262 - ABIE53_003375 0.81 -1.9 3,798,262 - ABIE53_003375 0.81 +3.2 3,798,323 + ABIE53_003375 0.86 -0.4 3,798,323 + ABIE53_003375 0.86 -0.7 3,798,323 + ABIE53_003375 0.86 +1.1 3,798,324 - ABIE53_003375 0.86 +1.2 3,798,324 - ABIE53_003375 0.86 -1.5 3,798,324 - ABIE53_003375 0.86 +1.1 3,798,345 - ABIE53_003375 0.87 -0.4 3,798,345 - ABIE53_003375 0.87 +0.5 3,798,345 - ABIE53_003375 0.87 +0.0 3,798,358 + ABIE53_003375 0.88 -0.1 3,798,358 + ABIE53_003375 0.88 +0.2 3,798,358 + ABIE53_003375 0.88 -0.9 3,798,359 - ABIE53_003375 0.88 -0.5 3,798,406 - -1.4 3,798,406 - +0.6 3,798,406 - +0.3 3,798,416 + -1.5 3,798,455 - -0.2 3,798,455 - +2.6 3,798,455 - +0.0 3,798,519 + +0.2 3,798,519 + -0.2 3,798,519 + +0.1 3,798,519 + +0.6 3,798,520 - -0.7 3,798,520 - +1.0 3,798,520 - +0.4 3,798,520 - +0.2 3,798,520 - +0.0 3,798,520 - +0.1 3,798,520 - +0.6 3,798,520 - +0.0 3,798,520 - +0.2 3,798,520 - -0.4 3,798,836 + ABIE53_003376 0.13 -1.2 3,798,836 + ABIE53_003376 0.13 -1.2 3,798,836 + ABIE53_003376 0.13 -1.0 3,798,836 + ABIE53_003376 0.13 +0.5 3,798,836 + ABIE53_003376 0.13 +0.1 3,798,836 + ABIE53_003376 0.13 -1.5 3,798,837 - ABIE53_003376 0.13 +0.3 3,798,837 - ABIE53_003376 0.13 +0.3 3,798,837 - ABIE53_003376 0.13 +1.2 3,798,837 - ABIE53_003376 0.13 +0.1 3,798,837 - ABIE53_003376 0.13 -0.7 3,798,837 - ABIE53_003376 0.13 +0.8 3,798,837 - ABIE53_003376 0.13 +0.2 3,798,863 + ABIE53_003376 0.18 +0.3 3,798,864 - ABIE53_003376 0.18 +0.7 3,798,864 - ABIE53_003376 0.18 +0.1 3,798,864 - ABIE53_003376 0.18 +0.7 3,798,875 + ABIE53_003376 0.20 -0.4 3,798,875 + ABIE53_003376 0.20 +2.6 3,798,875 + ABIE53_003376 0.20 +0.7 3,798,875 + ABIE53_003376 0.20 -0.9 3,798,875 + ABIE53_003376 0.20 +1.5 3,798,875 + ABIE53_003376 0.20 +0.5 3,798,876 - ABIE53_003376 0.21 +0.1 3,798,876 - ABIE53_003376 0.21 +1.3 3,798,876 - ABIE53_003376 0.21 +0.3 3,798,893 + ABIE53_003376 0.24 +0.0 3,798,893 + ABIE53_003376 0.24 +0.1 3,798,893 + ABIE53_003376 0.24 -1.3 3,798,893 + ABIE53_003376 0.24 -0.2 3,798,894 - ABIE53_003376 0.24 -2.1 3,798,894 - ABIE53_003376 0.24 +0.3 3,798,894 - ABIE53_003376 0.24 +0.1 3,798,894 - ABIE53_003376 0.24 -1.2 3,798,894 - ABIE53_003376 0.24 +0.2 3,799,023 - ABIE53_003376 0.47 -0.6 3,799,023 - ABIE53_003376 0.47 +0.6 3,799,041 + ABIE53_003376 0.51 -0.7 3,799,042 - ABIE53_003376 0.51 -0.1 3,799,042 - ABIE53_003376 0.51 -2.1 3,799,042 - ABIE53_003376 0.51 +0.0 3,799,042 - ABIE53_003376 0.51 +0.4 3,799,042 - ABIE53_003376 0.51 -0.5 3,799,103 + ABIE53_003376 0.62 -0.3 3,799,103 + ABIE53_003376 0.62 +1.0 3,799,103 + ABIE53_003376 0.62 +0.0 3,799,104 - ABIE53_003376 0.62 +0.3 3,799,104 - ABIE53_003376 0.62 -1.7 3,799,178 + ABIE53_003376 0.76 +0.7 3,799,178 + ABIE53_003376 0.76 -1.2 3,799,178 + ABIE53_003376 0.76 +0.0 3,799,178 + ABIE53_003376 0.76 +1.8 3,799,178 + ABIE53_003376 0.76 -1.1 3,799,179 - ABIE53_003376 0.76 -1.1 3,799,179 - ABIE53_003376 0.76 +2.0 3,799,179 - ABIE53_003376 0.76 +0.5 3,799,179 - ABIE53_003376 0.76 +0.6 3,799,247 + ABIE53_003376 0.88 +1.0 3,799,248 - ABIE53_003376 0.88 +0.2 3,799,248 - ABIE53_003376 0.88 -0.2 3,799,248 - ABIE53_003376 0.88 +0.1 3,799,385 + +0.6 3,799,385 + +0.6 3,799,399 - +1.9 3,799,454 - -0.3 3,799,454 - +0.6 3,799,482 + +1.1 3,799,482 + +0.3 3,799,483 - -0.7 3,799,483 - -0.7 3,799,791 + ABIE53_003377 0.44 +0.1 3,799,791 + ABIE53_003377 0.44 -0.4 3,799,791 + ABIE53_003377 0.44 -0.1 3,799,791 + ABIE53_003377 0.44 +0.3 3,799,791 + ABIE53_003377 0.44 -0.7 3,799,791 + ABIE53_003377 0.44 +0.1 3,799,792 - ABIE53_003377 0.44 +3.1 3,799,792 - ABIE53_003377 0.44 -0.2 3,799,792 - ABIE53_003377 0.44 -0.0 3,799,792 - ABIE53_003377 0.44 +3.6 3,799,792 - ABIE53_003377 0.44 -0.2 3,799,792 - ABIE53_003377 0.44 +0.4 3,799,792 - ABIE53_003377 0.44 -0.8 3,799,792 - ABIE53_003377 0.44 +0.6 3,799,828 + ABIE53_003377 0.51 -1.2 3,799,828 + ABIE53_003377 0.51 +0.2 3,799,828 + ABIE53_003377 0.51 -1.7 3,799,828 + ABIE53_003377 0.51 +0.7 3,799,828 + ABIE53_003377 0.51 -0.4 3,799,828 + ABIE53_003377 0.51 -0.2 3,799,828 + ABIE53_003377 0.51 +0.6 3,799,828 + ABIE53_003377 0.51 -0.5 3,799,828 + ABIE53_003377 0.51 -1.4 3,799,828 + ABIE53_003377 0.51 -0.6 3,799,828 + ABIE53_003377 0.51 +1.2 3,799,828 + ABIE53_003377 0.51 +2.1 3,799,828 + ABIE53_003377 0.51 +0.5 3,799,828 + ABIE53_003377 0.51 -0.3 3,799,828 + ABIE53_003377 0.51 -0.7 3,799,829 - ABIE53_003377 0.51 +2.2 3,799,829 - ABIE53_003377 0.51 +0.1 3,799,829 - ABIE53_003377 0.51 +0.3 3,799,829 - ABIE53_003377 0.51 +1.1 3,799,829 - ABIE53_003377 0.51 +0.6 3,799,829 - ABIE53_003377 0.51 +0.0 3,799,829 - ABIE53_003377 0.51 +0.6 3,799,873 + ABIE53_003377 0.60 +0.1 3,799,874 - ABIE53_003377 0.60 +0.8 3,799,874 - ABIE53_003377 0.60 +0.5 3,799,898 + ABIE53_003377 0.65 -1.4 3,799,940 + ABIE53_003377 0.73 +0.3 3,799,953 + ABIE53_003377 0.75 +0.3 3,800,076 + -0.4 3,800,076 + +0.7 3,800,077 - +0.4 3,800,077 - +1.4 3,800,077 - -0.1 3,800,077 - -0.1 3,800,077 - -0.3 3,800,077 - +0.5 3,800,077 - -0.5 3,800,077 - -1.5 3,800,234 + ABIE53_003378 0.18 +1.5 3,800,234 + ABIE53_003378 0.18 +0.8 3,800,235 - ABIE53_003378 0.18 +0.3 3,800,235 - ABIE53_003378 0.18 -0.0 3,800,246 + ABIE53_003378 0.20 +0.7 3,800,247 - ABIE53_003378 0.20 +0.6 3,800,267 + ABIE53_003378 0.24 +0.7 3,800,267 + ABIE53_003378 0.24 +0.6 3,800,267 + ABIE53_003378 0.24 -0.2 3,800,267 + ABIE53_003378 0.24 -1.9 3,800,267 + ABIE53_003378 0.24 +0.3 3,800,268 - ABIE53_003378 0.24 +1.1 3,800,268 - ABIE53_003378 0.24 +0.6 3,800,269 + ABIE53_003378 0.25 +0.5 3,800,269 + ABIE53_003378 0.25 +1.6 3,800,269 + ABIE53_003378 0.25 -0.1 3,800,269 + ABIE53_003378 0.25 +0.2 3,800,269 + ABIE53_003378 0.25 +0.3 3,800,269 + ABIE53_003378 0.25 +0.5 3,800,269 + ABIE53_003378 0.25 +0.6 3,800,269 + ABIE53_003378 0.25 +0.3 3,800,269 + ABIE53_003378 0.25 +0.6 3,800,269 + ABIE53_003378 0.25 +0.2 3,800,270 - ABIE53_003378 0.25 -0.4 3,800,270 - ABIE53_003378 0.25 +3.2 3,800,270 - ABIE53_003378 0.25 +0.9 3,800,270 - ABIE53_003378 0.25 +0.4 3,800,296 + ABIE53_003378 0.30 -0.4 3,800,296 + ABIE53_003378 0.30 +0.6 3,800,296 + ABIE53_003378 0.30 -0.7 3,800,296 + ABIE53_003378 0.30 +1.3 3,800,296 + ABIE53_003378 0.30 +0.6
Or see this region's nucleotide sequence