Experiment: Varel_Bryant_medium_Glucose with Bile salts 0.5 mg/mL; pH 7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0246 and BT0247 are separated by 53 nucleotides BT0247 and BT0248 are separated by 6 nucleotides BT0248 and BT0249 are separated by 69 nucleotides BT0249 and BT0250 are separated by 25 nucleotides
BT0246: BT0246 - putative integral membrane protein, with calcineurin-like phosphoesterase domain (NCBI ptt file), at 272,167 to 273,333
BT0246
BT0247: BT0247 - hypothetical protein (NCBI ptt file), at 273,387 to 273,965
BT0247
BT0248: BT0248 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 273,972 to 274,481
BT0248
BT0249: BT0249 - 5-methyltetrahydrofolate--homocysteine methyltransferase or methionine synthase (NCBI ptt file), at 274,551 to 275,453
BT0249
BT0250: BT0250 - dihydroorotase (DHOase) (NCBI ptt file), at 275,479 to 276,816
BT0250
Position (kb) 273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 272.978 kb on - strand, within BT0246 at 273.182 kb on + strand, within BT0246 at 273.279 kb on - strand at 273.333 kb on + strand at 273.405 kb on - strand at 273.974 kb on - strand at 274.008 kb on - strand at 274.097 kb on + strand, within BT0248 at 274.097 kb on + strand, within BT0248 at 274.097 kb on + strand, within BT0248 at 274.179 kb on + strand, within BT0248 at 274.348 kb on + strand, within BT0248 at 274.379 kb on + strand, within BT0248 at 274.380 kb on - strand, within BT0248 at 274.383 kb on + strand, within BT0248 at 274.387 kb on + strand, within BT0248 at 274.387 kb on + strand, within BT0248 at 274.392 kb on + strand, within BT0248 at 274.392 kb on + strand, within BT0248 at 274.392 kb on + strand, within BT0248 at 274.392 kb on + strand, within BT0248 at 274.393 kb on - strand, within BT0248 at 274.542 kb on - strand at 274.616 kb on - strand at 274.831 kb on + strand, within BT0249 at 274.899 kb on + strand, within BT0249 at 274.899 kb on + strand, within BT0249 at 274.903 kb on + strand, within BT0249 at 275.011 kb on - strand, within BT0249 at 275.011 kb on - strand, within BT0249 at 275.172 kb on - strand, within BT0249 at 275.402 kb on - strand at 275.434 kb on - strand at 275.438 kb on + strand at 275.438 kb on + strand at 275.439 kb on - strand at 275.439 kb on - strand at 275.439 kb on - strand at 275.453 kb on + strand at 275.454 kb on - strand at 275.459 kb on + strand at 275.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Bile salts 0.5 mg/mL; pH 7 remove 272,978 - BT0246 0.69 -2.0 273,182 + BT0246 0.87 +0.2 273,279 - +0.4 273,333 + -1.4 273,405 - -1.2 273,974 - +2.0 274,008 - -2.0 274,097 + BT0248 0.25 +0.9 274,097 + BT0248 0.25 +1.0 274,097 + BT0248 0.25 +0.7 274,179 + BT0248 0.41 +0.1 274,348 + BT0248 0.74 -0.3 274,379 + BT0248 0.80 -0.6 274,380 - BT0248 0.80 +1.9 274,383 + BT0248 0.81 +0.0 274,387 + BT0248 0.81 -2.0 274,387 + BT0248 0.81 -0.6 274,392 + BT0248 0.82 -2.0 274,392 + BT0248 0.82 +2.0 274,392 + BT0248 0.82 -1.2 274,392 + BT0248 0.82 -2.0 274,393 - BT0248 0.83 -2.9 274,542 - +0.4 274,616 - -0.5 274,831 + BT0249 0.31 +0.5 274,899 + BT0249 0.39 -0.6 274,899 + BT0249 0.39 +0.6 274,903 + BT0249 0.39 -0.6 275,011 - BT0249 0.51 +0.2 275,011 - BT0249 0.51 -1.3 275,172 - BT0249 0.69 -0.6 275,402 - -0.6 275,434 - -2.4 275,438 + +0.4 275,438 + -0.2 275,439 - +0.4 275,439 - +0.7 275,439 - -1.8 275,453 + +1.0 275,454 - -2.1 275,459 + +1.7 275,460 - +0.4
Or see this region's nucleotide sequence